Team:Technion-Israel/Parts
Our team designed two sets of vectors- the first set includes three vectors that were designed for protein purification, both from yeast and bacteria, while the other set includes six vectors for surface display in Bacillus subtilis spores. In the first set, one vector was designed for the extraction of ACE2 from yeast, while the other two were for the extraction of Sybodies 15 and 68 from E.coli. The second set includes two different coat proteins (CotC and CotG) for displaying three proteins that were mentioned before (ACE2, Sybody 15 and Sybody 68).
This is a list of our parts:
| Name | Type | Description | Length [bp] |
|---|---|---|---|
| BBa_K3653000 | Coding | Human Angiotensin-converting enzyme 2 (hACE2) is an enzyme that is attached to the cell membranes of cells in the lungs, arteries, heart, kidneys, and intestines. | 2415 |
| BBa_K3653001 | Regulatory | T7 promoter allows over-expression of proteins in E.coli that express T7 RNA polymerase. | 19 |
| BBa_K3653002 | Coding | Sybody 15 is a synthetic nanobody that targets SARS-CoV-2 spike proteins. | 388 |
| BBa_K3653003 | Coding | Sybody 68 is a synthetic nanobody that targets SARS-CoV-2 spike proteins. | 418 |
| BBa_K3653004 | Plasmid backbone | pET9D is a bacterial expression vector with KAN antibiotic resistance and T7 promoter. | 4338 |
| BBa_K3653005 | Plasmid backbone | p416-FEC-mCore1 is a high copy number yeast expression vector. It can also be propagated in E.coli, hence it is a shuttle vector. | 5985 |
| BBa_K3653006 | Coding | Cot C is a spore coat proteins that are located in the outermost layer of the coat. Those proteins are used for display of heterologous proteins. | 198 |
| BBa_K3653007 | Coding | Cot G is a spore coat proteins that are located in the outermost layer of the coat. Those proteins are used for display of heterologous proteins. | 582 |
| BBa_K3653008 | Plasmid backbone | pBSC1_Lacz is a backbone that can be linearized and integrated into the α-amylase (amyE) locus in B.subtilis. | 10971 |
| BBa_K3653009 | Coding | Human ACE2 (after codon optimization for B.Subtilis) - Angiotensin-converting enzyme 2 is an enzyme attached to the cell membranes of cells in the lungs, arteries, heart, kidneys, and intestines. | 2448 |
| BBa_K3653021 | Plasmid backbones | p416-FEC-mCore1 is a high copy number yeast expression vector. It can also be propagated in E.coli, hence it is a shuttle vector. The addition of the His-tag allows protein purification. The target protein is the WT Angiotensin-converting enzyme 2. | 7696 |
| BBa_K3653011 | Plasmid backbones | p416-FEC-mCore1 with His-tag and Angiotensin-converting enzyme 2 mutation 1 is a high copy number yeast expression vector. It can also be propagated in E.coli, hence it is a shuttle vector. The addition of the His-tag allows protein purification. The target protein is a mutated Angiotensin-converting enzyme 2. | 7696 |
| BBa_K3653012 | Plasmid backbones | p416-FEC-mCore1 with His-tag and Angiotensin-converting enzyme 2 mutation 2 is a high copy number yeast expression vector. It can also be propagated in E.coli, hence it is a shuttle vector. The addition of the His-tag allows protein purification. The target protein is a mutated Angiotensin-converting enzyme 2. | 7696 |
| BBa_K3653013 | Plasmid backbones | pET9D backbone with Sybody 15 - pET9D is bacterial expression vector with KAN antibiotic resistance and T7 promoter. Sybody 15 is a nanobody that targets SARS-CoV-2 spike proteins. | 4674 |
| BBa_K3653014 | Plasmid backbones | pET9D backbone with Sybody 68 - pET9D is bacterial expression vector with KAN antibiotic resistance and T7 promoter. Sybody 68 is a nanobody that targets SARS-CoV-2 spike proteins. | 4704 |
| BBa_K3653015 | Plasmid backbone | pBS1C_CotG_Sybody15 - A fusion of a backbone vector (pBS1C) that can be integrated into the α‐amylase (amyE) locus in B.subtilis, along with a sequence encoding the spore surface coat protein (CotG) that is followed by a sequence encoding the heterologous protein (Sybody 15). | 7788 |
| BBa_K3653016 | Plasmid backbone | pBS1C_CotG_Sybody68 - A fusion of a backbone vector (pBS1C) that can be integrated into the α‐amylase (amyE) locus in B.subtilis, along with a sequence encoding the spore surface coat protein (CotG) that is followed by a sequence encoding the heterologous protein (Sybody 68). | 7818 |
| BBa_K3653017 | Plasmid backbone | pBS1C_CotG_ACE2 - The final plasmid which can be integrated into the amyE locus of Bacillus subtilis.The sequence that undergoes genome integration contains a fusion of a surface spore coat protein (CotG) with the heterologous protein (ACE2). | 9858 |
| BBa_K3653018 | Plasmid backbone | pBS1C_CotC_Sybody15 - The final plasmid which can be integrated into the amyE locus of Bacillus subtilis.The sequence that undergoes genome integration contains a fusion of a surface spore coat protein (CotC) with the heterologous protein (Sybody15). | 7391 |
| BBa_K3653019 | Plasmid backbone | pBS1C_CotC_Sybody68 - A fusion of a backbone vector (pBS1C) that can be integrated into the α‐amylase (amyE) locus in B.subtilis, along with a sequence encoding the spore surface coat protein (CotC) that is followed by a sequence encoding the heterologous protein (Sybody 68). | 7421 |
| BBa_K3653020 | Plasmid backbone | pBS1C_CotG_ACE2 - A fusion of a backbone vector (pBS1C) that can be integrated into the α‐amylase (amyE) locus in B.subtilis, along with a sequence encoding the spore surface coat protein (CotG) that is followed by a sequence encoding the heterologous protein (ACE2). | 9461 |
