This page summarizes the procedure to build a DIY turbidostat and gives some calibration documents that may be helpful for future teams. Our whole philosophy is to promote the open-source hardware movement and that is why we compiled this manual, providing a useful guide for future igemers willing to build a DIY turbidostat. Hope you find it useful!
DIY turbidostat manual
Main parts list:
- 3D printed piece
- Aluminum tube (exterior diameter of 32mm, interior diameter of 28mm, 8cm height)
- Methacrylate sheet (laser cut parts, 2 pieces of 4cm x 4cm)
- Nuts and screws
- Glass wide-mouth bottle (x2)
- Glass vial (diameter of 28mm)
- ArduinoTM microcontroller
- General printed circuit board
- Connection cables
- Magnets (x2)
- Stirring magnets
- Heating resistors (x2)
- LED (600nm)
- 12V fan
- 12V power supply
- Peristaltic pump (x2)
1 Design a 3D printing piece for the turbidostat tube holder.
- It should have two small holes forming 135º. One hole will correspond to the placement of the LED and the other to the placement of the photodiode. Therefore, the dimensions of these 2 components should be taken into account for the design of the small holes.
- Its main hole diameter should match with the exterior diameter of the aluminum tube. And, at the same time, the interior diameter of the aluminum tube should match the glass vial diameter.
2 Laser cut a methacrylate sheet.
3 Drill the aluminum tube forming the two small holes that will match with the ones from the 3D printed piece.
4 Perform a hole on a general printed circuit board with a diameter equal to the external diameter of the aluminum tube (32mm).
5 Fix the 2 magnets to the 12V fan with silicone.
6 Assemble the sleeve: put together the 12V fan (with the magnets), the laser cut parts and the 3D printed piece by the insertion of the screws across the mini-holes on corners. Place the aluminum tube and the general printed circuit board. Put the glass vial inside the aluminum tube.
In order to make a working turbidostat, two key areas need to be properly designed.
- Electronics module: there are several electronic circuits underlying the turbidostat, all of which are explained in detail in the Hardware page.
- Software module: once all the background information about the turbidostat code programming is understood, extensively explained in the Software page, you can check our designed open-source code here.
Learn how to establish a relationship between the measured ArduinoTM analog OD value and the real OD.
Learn how to obtain the minimum volume of cell culture so that it ensures a robust turbidostat control.
Learn how to establish a relationship between the ArduinoTM analog temperature value and the real one.