Team:WHU-China/Contribution
We’re proud to introduce our contributions this year for future iGEMers in multiple directions, with detailed documentations in respective pages.
Wet-lab: Despite only one month and a half to perform experiments, we still tried hard for useful experimental data, finally submitting several new parts to the Registry with corresponding documentations, from BBa_K3562000 to BBa_K3562015, including a part collection of chemokines. Also, we added information supported by literatures to existing parts, including BBa_C0160, BBa_K3562001, BBa_K855004, etc.
Mostly away from laboratories, we focused more on dry lab work this year and endeavoured to implement several dry-lab outcomes to wet lab in a rather limited time.
Hardware: We thoroughly documented the design and production process of our DIY co-culture device, with supplementary teaching videos provided. The EcN-and-P. aeruginosa co-culture experiments were performed on the co-culture device to prove its feasibility. As for our microfluidic chips, we established long-time connections with emulatebio, an enterprise specilized in organ-simulating chips. Also, we documented the 3D printing process of a part of our microfluidics.
Model: The abundant potentials to motivate our project by modelling offered us conveniences, and we’re delighted to share our modelling experiences with future iGEMers. For instance, our work on ancestral proteins reconstitution and structural analysis gave innovative insights into evolution of enzymes and corresponding applications. In addition, we utilized softwares gro and simbiology in our quorum sensing models, inspired by 2016 Imperial, and the outcomes helped to deepen our understanding of Quenching Module. Please see our Model page for more detailed information.
To achieve the pursuits of iGEM, i.e. respect, community and honesty, we conducted the work of Human Practices and Entrepreneurship in collaborations with various iGEM teams.
Human Practices: It’s our pleasure to lead Anti-Biofilm Community this year to produce a meaningful educational brochure, ‘A Handbook of Anti-Biofilm Community’, and distribute it online and offline to interested people. In addition, we proposed a method to create integrated questionnaires, with enhanced interactions explored between iGEMers and society. Briefly, our team this year fulfilled the goals of inclusivity and education in a reciprocal way.
Entrepreneurship: It’s our pleasure to lead China Synthetic Biotic Delta for surveying the biotech future of Wuhan, Shanghai and Beijing, and develop ‘a collaborative manuscript for engineered probiotic start-ups’ for individualized business plans for engineered probiotics with applications into different contexts.
