Team:WHU-China/Inclusion

Proof-of-Concept

Group Interview

Besides top experts, we also interviewed common people that we’re most familiar with-our family members from all walks of life, including emergency center directors, nurses, police officers and other grass-roots staff, hoping to comprehensively understand the current situation of nosocomial infections in grassroots hospitals as well as the influenced citizen lifestyles under the pressure of coronavirus.

About Nosocomial Infection

Nosocomial infection is so common that almost all patients in the long period of relabilitation or coma have a chance to have acquired pneumonia, and the infection is mostly mixed type. Once the infection occurs, anti-infection treatment will be very tough. Acquired pneumonia has always been a core topic in the ICU.


The pulmonary nosocomial infection has a direct connection to the use of the ventilator, which is usually used to create an artificial airway. It is done through endotracheal intubation and tracheotomy, during which there are three possible ways in which infection can occur:

  • The establishment of artificial airways has direct a destruction to the airway barrier;

  • The process of using ventilators includes heating and humidification, which may affect the movement of cilia and airway self-purification because it's difficult for the machine to fully simulate human body, so the process;

  • Sputum aspiration and other invasive operations may induce infection. In order to prevent infection caused by pathogens directly carried by the ventilator, the machine will be regularly cleaned and maintained.


  • In addition to the use of ventilators, there are many other factors that lead to pulmonary nosocomial infection:

  • The respiratory tract itself is open, prone to infection;

  • In the epidemic, the large number of patients and suspected cases caused a run on medical resources. The condition did not allow all the equipment to be single-use, so many instruments had to be reused, which without doubt increased the probability of iatrogenic infection.


  • At present, Pseudomonas aeruginosa ranks the top three in nosocomial infection in China. Antibiotics are included in the main therapy, but there is a certain degree of drug resistance. Therefore, these antibiotics often only play some basic roles. Combined use of antibiotics and development of new antibiotics can partly slow down the emergence of drug resistance, but combined use will also affect the function of organs such as liver and kidney. In addition to antibiotics, it is also necessary to give patients nutritional supplements to improve their immunity.


    About Clinical Application of Engineered Probiotics

  • At present, few engineered bacteria are used in the treatment of pulmonary infection. The usage is mainly focused on intestinal probiotics.

  • At present, the problem of how to transport the engineered bacteria to the destination needs to be solved before the application of engineered probiotics in lungs.


  • About Front-line Fighters against COVID-19

    In the face of the sudden outbreak of COVID-19, front-line medical workers are faced with the difficulties of insufficient medical resources, shortage of hands and extremely high risk of infection, but they are still came to from all directions to help Wuhan. Under the epidemic situation, there were more social destablizing factors. When most people could keep themselves in quarantine at home, apart from medical workers, there were also groups of people who were going backwards regardless the threat of the disease. The control of the community, the social follow-up of migrants and vehicles, the crackdown on cases against the anti-epidemic regulations, the protection of the basic living needs of vulnerable groups, the maintenance of market order, the protection of rights and interests of citizens, etc. They stick to their positions like screws, keeping the social machine running steadily. Thanks to them, we were able to adjust to the onslaught of COVID-19 and quickly get back on track.


    Questionnaires and analysis
    Overview

    To obtain broad and distinct insights around the major social concerns that our project encompasses, e.g. nosocomial infection and engineered probiotic, we distributed two questionnaires with exquisitely designed questions to the community via online and offline approaches, such as social media and lecture. More than 500 answers were collected and then analyzed. We believe that the outcomes of these questionnaires not only provided us with public recognition scope of project-related themes, but pushed forward our project in different perspectives, such as inclusivity, education and entrepreneurship.

    Strategy

    Our aim is to develop ‘integrated questionnaires’ (just like integrated human practices), which facilitate sufficient and efficient communications between us and the community. Notably, when delivering the questionnaires, we also wrote supplementary articles for education. We split the questionnaire into three sections, which include basic information, public recognition of project-related areas, and outreach questions. In Part I: Basic Information, only three necessary questions were proposed to distinguish the participants, in case that the redundancy of basic questions might lessen their willingness to accomplish our survey. In Part II, to enable quantification of participants’ understanding of ‘nosocomial infection’ and ‘engineered probiotic’, we designed a considerable proportion of numerical rating scale in our survey, instead of those ‘yes/no’ questions. Importantly, in the last section of outreach questions, several interactive questions were designed to guide the way to directly develop our project, for instance, statistical results of the ranking ‘the appropriate forms to spread relevant knowledges:’ played a decisive role in the decision-making process of our science-without-border activities.

    Analysis

    Using the data generated from two surveys, we performed statistical analysis and those outcomes indicated the direction for us to advance. Several critical points are described whereafter:

  • About 90% of answers were derived from teenagers and young adults (aging 13 to 25), thus unable to represent the whole scope of public recognition. Accordingly, we modified our ensuing HP strategy for more diverse ages.
  • Based on the second ‘yes/no’ question, we classified answers into two groups, i.e. people with or without a biology/medicine background. Interestingly, the public recognition towards several topics vary in two groups, and for another cases, no significant difference was observed.

  • Question 6 of engineered probiotic survey: ‘Prevent purchasing transgenic products in daily life.'


    Question 15 of engineered probiotic survey: ‘Your general acceptance degree of engineered probiotics implemented in biomedical fields:’


    Question 12 of nosocomial infection survey: ‘Intensive care units are germ-free.’


    Question 16 of nosocomial infection survey: ‘Your general judgment towards nosocomial infections during the epidemic:’

  • Determined by the results of the relevant questions, we chose comics as the major component of our educational activities.
  • Question 18 of nosocomial infection survey: ‘Do you think nosocomial infection-related introductions are necessary? ’


    Question 19 of nosocomial infection survey: ‘Do you think these science communication activities will help improve the ongoing situations?’


    Question 20 of nosocomial infection survey: ‘The appropriate forms to spread relevant knowledges:’

  • We’ve collected a large number of suggestions on entrepreneurship, which subsequently contributed to our business plan.
  • This cloud art created by wordart.com was derived from answers to question 19 of engineered probiotic survey, ‘In your opinion, the limiting factors that hamper the development of China bio-tech enterprises could involve:’.

    In summary, we anticipate that our project will greatly benefit from the integrated questionnaires. Download PDF version of our questionnaires here:

    Online Campaign of #FindBiofilms

    Driven by the collaborative principles of Anti-Biofilm Community, we participated in the online campaign of #FindBiofilms, following the proposer UM_Macau. Aiming to spread the concepts of biofilms together with the detrimental impact of biofilm formation, the activity was held in different social media, such as WeChat public official account, Facebook and Instagram, for collecting daily-life pictures of biofilm-like items from diverse groups. After selection, UM_Macau displayed them in ‘A Handbook of Anti-Biofilm Community’ to fulfill education goals.