Team:WHU-China/Parts
Although wet lab work is relatively limited for us this year, we still use and construct several parts which are considered as an important part of our project. To efficiently obtain these parts, we asked two companies, IDT and GenScript, to synthesize some of them and send to us. Besides, we also used colony PCR to get several parts from the original bacteria strain directly. As for the parts of chemokines, we bought the products of them and characterized them directly. However, due to the limited time for experiments, we didn’t get all of these parts and enough data for those we got. And that is also why we haven’t submitted composite parts this year. Nevertheless, we are still glad to share what we got with other teams so we have submitted all our new basic parts and the data we obtained to the registry and we have constructed a part collection composed of seven parts of chemokines (with related data) for future teams to use!
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_I746361 | PO promoter | Promoter | 92bp |
| BBa_K649000 | las promoter | Promoter | 83bp |
| BBa_R0071 | rhl promoter | Promoter | 53bp |
| BBa_J23100 | Constitutive promoter | Promoter | 35bp |
| BBa_K2965011 | Enhanced T7 promoter 1 | Promoter | 23bp |
| BBa_K3562002 | Promoter activated by LasR/AHL complex (pLasRV) | Promoter | 155bp |
| BBa_B0030 | RBS.1(strong) | RBS | 15bp |
| BBa_B0034 | RBS based on Elowitz repressilator | RBS | 12bp |
| BBa_B1002 | Terminator | Terminator | 34bp |
| BBa_B0010 | T1 from E. coli rrnB | Terminator | 80bp |
| BBa_C0160 | autoinducer inactivation enzyme aiiA (no LVA) | Coding | 756bp |
| BBa_K855004 | pvdQ gene form pseudomonas aeruginosa PAO1 with his tag added before stop codon | Coding | 2307bp |
| BBa_K3562000 | Acylase coding gene with His tag (AiiC) | Coding | 2565bp |
| BBa_K3562001 | Acylase coding gene with His tag (AiiO) | Coding | 837bp |
| BBa_K1157001 | Quorum sensing receptor (PQSR) | Coding | 999bp |
| BBa_I746352 | delta activator from phiR73 phage (phiR73 delta) | Translational_Unit | 264bp |
| BBa_C0179 | LasR activator (LasR) | Coding | 723bp |
| BBa_C0178 | Autoinducer synthetase (LasI) | Coding | 609bp |
| BBa_C0171 | rhlR repressor/activator (RhlR) | Coding | 729bp |
| BBa_C0170 | Autoinducer synthetase for BHL and HHL (RhlI) | Coding | 609bp |
| BBa_E0040 | Wild-type GFP (GFP mut3b) | Coding | 720bp |
| BBa_J06504 | monomeric RFP optimized for bacteria(mCherry) | Reporter | 714bp |
| BBa_K302032 | Antitoxin coding gene(mazE) | Coding | 249bp |
| BBa_K1075037 | Toxin coding gene and and terminator(mazF-TT) | Intermediate | 476bp |
| BBa_K831001 | Antitoxin coding gene (HipB) | Coding | 267bp |
| BBa_K3562005 | Toxin coding gene (HipA) | Coding | 1323bp |
| BBa_K3562003 | Antitoxin coding gene (PemI) | Coding | 258bp |
| BBa_K3562004 | Toxin coding gene (PemK) | Coding | 402bp |
| BBa_K3562006 | Antitoxin coding gene (sokA) | Coding | 30bp |
| BBa_K3562007 | Toxin coding gene (hokA) | Coding | 153bp |
| BBa_K3562008 | Antitoxin coding gene (ToxI) | Coding | 252bp |
| BBa_K3562009 | Toxin coding gene (ToxN) | Coding | 516bp |
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K2957001 | Chemokine coding gene with FLAG tag (IL-8-FLAg tag) | Coding | 338bp |
| BBa_K3562010 | Chemokine coding gene (CXCL-1) | Coding | 219bp |
| BBa_K3562011 | Chemokine coding gene (CXCL-2) | Coding | 219bp |
| BBa_K3562012 | Chemokine coding gene (CXCL-3) | Coding | 219bp |
| BBa_K3562013 | Chemokine coding gene (CCL-2) | Coding | 228bp |
| BBa_K3562014 | Chemokine coding gene (CCL-3) | Coding | 207bp |
| BBa_K3562015 | Chemokine coding gene (CCL-8) | Coding | 228bp |
