Team:Worldshaper-Shanghai/Parts

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Summary

The goal of Worldshaper-Shanghai 2020 is to develop a new non-invasive technique for early stage prostate cancer diagnosis, mainly by expressing the color variation of the tested urine.
Among the 4 parts we submitted, PCA3 (BBa_K3577002) and KLK3 (BBa_K3577003) were urine biomarkers with great clinical potential, we aimed to detect these two biomarkers by gene amplication for prostate screening. The toehold swtich is responsible for providing a clear display of results. We constructed the composite part “T7-toehold-mCherry” (BBa_K3577000) as a regulator of protein expression, if the biomarkers is detected, the toehold switch will be activated to express red fluorescent protein. All proof data has been uploaded to the Part Registry page.

Parts

Name Type Descriptiont Designer Length
BBa_K3577000 Composite T7-toehold switch-mCherry Ruyi Shi 862
BBa_K3577001 Device Toehold Switch Ruyi Shi 84
BBa_K3577002 DNA PCA3 mRNA Xixi Song 196
BBa_K3577003 DNA KLK3 mRNA Xixi Song 382


Worldshaper-Shanghai 2020

New non-invasive technique for early stage prostate cancer diagnosis