Team:Worldshaper-Shanghai/Parts
Parts
Summary
The goal of Worldshaper-Shanghai 2020 is to develop a new non-invasive technique for early stage prostate cancer diagnosis, mainly by expressing the color variation of the tested urine.
Among the 4 parts we submitted, PCA3 (BBa_K3577002) and KLK3 (BBa_K3577003) were urine biomarkers with great clinical potential, we aimed to detect these two biomarkers by gene amplication for prostate screening. The toehold swtich is responsible for providing a clear display of results. We constructed the composite part “T7-toehold-mCherry” (BBa_K3577000) as a regulator of protein expression, if the biomarkers is detected, the toehold switch will be activated to express red fluorescent protein. All proof data has been uploaded to the Part Registry page.
Parts
| Name | Type | Descriptiont | Designer | Length |
| BBa_K3577000 | Composite | T7-toehold switch-mCherry | Ruyi Shi | 862 |
| BBa_K3577001 | Device | Toehold Switch | Ruyi Shi | 84 |
| BBa_K3577002 | DNA | PCA3 mRNA | Xixi Song | 196 |
| BBa_K3577003 | DNA | KLK3 mRNA | Xixi Song | 382 |
Worldshaper-Shanghai 2020
New non-invasive technique for early stage prostate cancer diagnosis