Team:XJTU-China/Part Collection
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XJTU-China
Part Collection
Overview
In order to increase the production of exopolysaccharides (EPSs), we altered the levels of enzymes in the central metabolism. Through a large number of literature investigations, we selected and over expressed two key enzymes, galU and pgmA.Then six composite parts have been constructed containing different combinations of extracellular polysaccharide synthesis gene galU and pgmA, to achieve maximal yield of extracellular polysaccharide, which is the key component of biological soil crusts for desertification control. Parts number ranges from BBa_K3331009 to BBa_K3331014.
In terms of biosafety, we have constructed a suicide switch controlled by pBAD. We found two sets of arabinose operon sequences, which were derived from Escherichia coli and Bacillus subtilis respectively. We constructed verification plasmids to verify their performance.
| Part Name | Basic/Composite | Number | Description |
| B.S.galU | Basic | K3331000 | Encodes Glucose-1-phosphate uridylyltransferase, or UGPase |
| E.coli galU | Basic | K3331001 | Encodes Glucose-2-phosphate uridylyltransferase, or UGPase |
| E.coli pgmA | Basic | K3331002 | Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase |
| B.S.pgmA | Basic | K3331003 | Encodes 2,4-bisphosphoglycerate-dependent phosphoglycerate mutase |
| P.A.pgmA | Basic | K3331004 | Encodes 2,5-bisphosphoglycerate-dependent phosphoglycerate mutase |
| nodA(MazF) | Basic | K3331005 | Encodes EndoA, an endoribonuclease that inactivates cellular mRNAs |
| PHT01 | Basic | K3331006 | It is a Escherichia coli-Bacillus subtilis shuttle plasmid. |
| araBAD terminator | Basic | K3331007 | This part is the terminator of ara operation in B.S |
| araC terminator | Basic | K3331008 | This part is the terminator of ara operation in E.coli. |
| B.S.galU+B.S.pgmA | Composite | K3331009 | The combination of two basic parts,B.S.galU and B.S.pgmA |
| B.S.galU+E.coli pgmA | Composite | K3331010 | The combination of two basic parts,B.S.galU and E.coli.pgmA |
| B.S.galU+P.A.pgmA | Composite | K3331011 | The combination of two basic parts,B.S.galU and P.A.pgmA |
| E.coli galU+E.coli pgmA | Composite | K3331012 | The combination of two basic parts,E.coli galU and E.coli pgmA |
| E.coli galU+B.S.pgmA | Composite | K3331013 | The combination of two basic parts, E.coli galU and B.S.pgmA |
| E.coli galU+P.A.pgmA | Composite | K3331014 | The combination of two basic parts,E.coli galU and P.A.pgmA |
| araBAD promoter | Basic | K3331015 | The promoter of araBAD. |
| E.coli BAD | Composite | K3331016 | This is the arabinose operon in the genome of E.coli. |
| E.coli BAD+GFP | Composite | K3331017 | This part can help us to test the expression efficiency of operons. |
| E.coli BAD+MazF | Composite | K3331018 | This is a suicide switch controlled by an arabinose operon. |
| araR Promoter | Basic | K3331020 | This part is the promoter of the arabinose operon repressor protein araR |
| araE Promoter | Basic | K3331021 | This part is the promoter of the arabinose operon repressor protein araE |
| araR | Basic | K3331022 | It expresses a regulatory protein araR, which inhibits the expression of the araE promoter |
| BS ara operon | Composite | K3331023 | It is the arabinose operon from Bacillus subtilis 168 genome, |
| BS ara operon&GFP | Composite | K3331024 | This part can help us to test the expression efficiency of operons. |
| BS ara operon&MazF | Composite | K3331025 | This is a suicide switch controlled by an arabinose operon. |