Team:XJTU-China/Collaborations
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XJTU-China
Collaboration
In the process of the competition, we have got support and help from many other teams. At the same time, we did our best to contribute to other teams, too. In this atmosphere of mutual help, we successfully completed our project and made many new friends. We also built up deep communication with some teams and established long-term cooperative partnerships.
Teams
NWU-CHINA-A
We had in-depth cooperation with NWU-China-A mainly in the field of mathematical modeling. The modeling leaders of our two teams helped each other to find relevant parameters of our modeling, modified the models mutually, and exchanged modeling methods and experience in real time during the project.
We also had extensive communication and cooperation in experimental methods, web page construction and submission.
NWU-CHINA-B
We provided NWU-China-B with three genes expressing linalool synthase: LGF, DHJ, RXY, and helped them to test the expression of UGT78D2 protein induced by different concentrations of IPTG.
We also have a long history of collaboration in mathematical modeling. The modeling leaders of the two teams helped each other find relevant modeling parameters, modified models, and shared modeling experience when the project progressing.
SZ-SHD
We provided them with a plasmid containing the GREEN fluorescent protein sfGFP gene. That fluorescent protein was used to test their UV switches, and we also provided relevant information such as the excitation wavelength of the fluorescent protein.
The Parts database was further improved by three teams in the northwest region: XJTU-China, NWU-China-A
As there are very few iGEM teams in northwest China, it is very inconvenient to seek help from nearby teams.Based on this, at the shaanxi gathering in 2019, we decided to establish a northwest database of common genes or parts of iGEM teams in northwest China for the convenience of future teams.This year we will continue to complete the five-year Northwest Alliance plan that we introduced last year.IGEM officials will provide free 20 KB-DNA synthesis per team per year, which we plan to use to build our parts library.This year, we continued to synthesize gene fragments of interest to form a DNA database about 120 KB in length.In addition, we will continue to enrich the database over the next few years to benefit teams in the northwest and across China.
Read Pdf
Meetups：
During the igem competition, in order to improve our project and find out the novel ideas of other iGEM teams, we participated in a series of Meetups.
Southern China Regional Online Meeting
On June 14th，Southern China Regional Online Meeting held by SZU-China. In this meetup We present one of the three alternatives to brainstorm – glowing plants. We carefully listened to the views and Suggestions of other teams on the significance, highlights and feasibility of our project. Although we had high hopes for the luminescent plant project at the beginning, our commitment to the project wavered in our communication with other teams. Other teams spoke highly of our idea, but their comments on the project cycle and other aspects prompted us to think more deeply about the feasibility of the project.
Shaanxi meet up
On July 25th, all of our team members participated in the discussion with NWU-China-A and NWU-China-B. This was the first time we had meetup since we confirmed the soil reconstruction project. In the communication, three of our teams put forward suggestions on each other’s project. We were surprised to find that the NWU-China-B project required linalool synthase, and we had its gene by chance, so after the meeting, we provided B with our sequence information and plasmid. We also asked NWU-China- A lots of questions about project modeling, and listened to students from Northwestern University
Southwest meetup
- On July 30, We participated in the Southwest meetup hosted by BUCT. Teams attending these meetups gave us a lot of plausible suggestions to improve our project. The most important one is to change the focus of our modeling work to the efficiency of the suicide switch. After this meetup, we deeply analyse the mechanism of the suicide system and established accurate model with amazing results.
Anti-escape system meetup
On August 2nd, we held a meetup on anti-escape systems and had an in-depth discussion with SCU-China and XMU-China on the necessity, ideas and methods of suicide switch design. In the discussion, we finally decided to design our suicide switch based on the arabinose operon.
the 7th Conference of China iGEMer Meetup
- We participated in the 7th CCIC. During the project presentation on August 29th, the judges raised many challenging questions for our project, which prompted us to think deeply about the project’s ideas and prove the feasibility of the project. After the meeting, we made great efforts to improve the relevant basis of our co-culture, which makes our project more reasonable.
