Difference between revisions of "Team:UCopenhagen/Partnership"

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<div><h1>pictures?</h1></div>
 
<div><h1>pictures?</h1></div>
  
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<div class="left-border-box">
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    <div class="box-flex-divider">
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        <div class="box-flex-small-first">
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        <img src="https://static.igem.org/mediawiki/2020/3/3b/T--UCopenhagen--flowline-blue-dot.png"> </div>
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        <div class="box-flex-big">
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              <div class="box-flex-text">
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                      <div>
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          <h2>1st meeting</h2>
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        </div>
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                    <div>
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blablalbalblablalbalbl
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                  </div>
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            </div>
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        </div>
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  </div>
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</div>
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<div class="right-border-box">
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    <div class="box-flex-divider">
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        <div class="box-flex-big">
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          <div class="box-flex-text">
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            <div>
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            <h2>Our Solution</h2>
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          </div>
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            <div> With CIDosis, we aim to improve the quality of life of patients, by providing a non-invasive device that will allow CID patients to monitor their own inflammation status from the comfort of their home. Our device consists of a patch that detects the levels of inflammatory markers in the sweat. This patch will be placed on the patient’s skin, where it will be continuously collecting sweat during the day, resulting in a color output reflecting the inflammatory status. The intensity of the color will correlate with the inflammation level. By employing a dedicated app, the patient will be able to track the color intensity using the camera of their mobile phone.  </div>
 +
          <div>
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Our self-monitoring patch, CIDosis, is an easy-to-use, non-invasive device, suitable for home use, and will decrease the need for frequent hospital visits,
 +
thereby making life easier for the patients. It allows for transparency for the patient regarding their disease progression and is a tool that can help them discover the best self-management strategy for their disease. Not only can it alleviate the burden of hospital visits, but it can provide valuable information on the therapeutic efficacy of certain drugs, allowing for faster treatment adjustments and continuous dosage regulations as the disease progresses. 
 +
</div>
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        <div> We envision the use of this patch as a future self-monitoring system for patients suffering from chronic inflammatory diseases worldwide. With the CIDosis patch they have access to a cheap, non-invasive method to measure their daily inflammation and track their condition in an easy way. The utility of such a device is extensive and can alleviate the struggles of many patient groups from Crohn’s disease to Rheumatoid Arthritis. </div>
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          </div>
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        </div>
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        <div class="box-flex-small-last">
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          <img src="https://static.igem.org/mediawiki/2020/3/3b/T--UCopenhagen--flowline-blue-dot.png">
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        </div>
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    </div>         
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</div>
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<div class="left-border-box">
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<div class="box-flex-divider">
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        <div class="box-flex-small-first">
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        <img src="https://static.igem.org/mediawiki/2020/3/3b/T--UCopenhagen--flowline-blue-dot.png">
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        </div>
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        <div class="box-flex-big">
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              <div class="box-flex-text">
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                      <div>
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                  <h2>Scientific approach</h2>
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                    </div>
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                    <div>
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Our project is founded on previous research showing the presence of interleukins IL-1β, IL-6 or IL-10 (ILs) in sweat [4,5,6], and the correlation with their respective levels in blood [5]. The CIDosis patch implementation is based on an engineered S. cerevisiae (baker’s yeast) biosensor capable of detecting the inflammation markers IL1, 6 and 10. The activation of the biosensor leads to the expression of a pigment biosynthesis gene and further to the accumulation of a colored compound. The biosensor will be located inside a patch specifically designed to sit on the skin and continuously collect sweat from the wearer for up to 24 hours. Once interleukins from the sweat reach the patch, our yeast receptors will sense their presence, and produce the aforementioned colored pigment corresponding to the level of the IL in question.
 +
</br></br>
 +
The biosensor design (Fig. 1) relies on the dimerization of modified human IL receptors on the yeast plasma membrane upon activation by their respective ligands. The modified receptors contain a synthetic transcription factor bound on their intracellular side that can be released upon dimerization of the receptors. Specifically, dimerization of the receptors leads to the co-localization of two halves of a split-actuator protein, which, when re- united, leads to cleavage of the protein resulting in the release of the transcription factor. The synthetic transcription factor, in turn, binds a specific synthetic promoter and finally activates the expression of the colorimetric reporter gene.
 +
</br></br>
 +
Our vision is a modular design, allowing for easy swap of the interleukin receptor in the yeast, so the patch can be tailored to the need of the individual patient group. The yeast is powered by designed receptors that boast very high sensitivity and selectivity, coupled with an efficient pheromone pathway for signal amplification. The SPY can be photographed with a specialized app to analyze the exact level of coloration regardless of light conditions. 
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  </div>
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<div class="lastbox">
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</br></br></br></br>
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</div>
  
 
<div><h1>Partnership Timeline</h1></div>
 
<div><h1>Partnership Timeline</h1></div>

Revision as of 14:31, 16 October 2020

Description Design Implementation Poster
Engineering Success Modeling/Simulation Parts Overview Results Protocols Notebook Safety
Integrated Human Practices Entrepreneurship Education
Collaborations Partnership Our Contribution
Judging Form Medals
Team Members Attributions Sponsors

Partnership

Partnership

Intro

pictures?

1st meeting

blablalbalblablalbalbl

Our Solution

With CIDosis, we aim to improve the quality of life of patients, by providing a non-invasive device that will allow CID patients to monitor their own inflammation status from the comfort of their home. Our device consists of a patch that detects the levels of inflammatory markers in the sweat. This patch will be placed on the patient’s skin, where it will be continuously collecting sweat during the day, resulting in a color output reflecting the inflammatory status. The intensity of the color will correlate with the inflammation level. By employing a dedicated app, the patient will be able to track the color intensity using the camera of their mobile phone.
Our self-monitoring patch, CIDosis, is an easy-to-use, non-invasive device, suitable for home use, and will decrease the need for frequent hospital visits, thereby making life easier for the patients. It allows for transparency for the patient regarding their disease progression and is a tool that can help them discover the best self-management strategy for their disease. Not only can it alleviate the burden of hospital visits, but it can provide valuable information on the therapeutic efficacy of certain drugs, allowing for faster treatment adjustments and continuous dosage regulations as the disease progresses.
We envision the use of this patch as a future self-monitoring system for patients suffering from chronic inflammatory diseases worldwide. With the CIDosis patch they have access to a cheap, non-invasive method to measure their daily inflammation and track their condition in an easy way. The utility of such a device is extensive and can alleviate the struggles of many patient groups from Crohn’s disease to Rheumatoid Arthritis.

Scientific approach

Our project is founded on previous research showing the presence of interleukins IL-1β, IL-6 or IL-10 (ILs) in sweat [4,5,6], and the correlation with their respective levels in blood [5]. The CIDosis patch implementation is based on an engineered S. cerevisiae (baker’s yeast) biosensor capable of detecting the inflammation markers IL1, 6 and 10. The activation of the biosensor leads to the expression of a pigment biosynthesis gene and further to the accumulation of a colored compound. The biosensor will be located inside a patch specifically designed to sit on the skin and continuously collect sweat from the wearer for up to 24 hours. Once interleukins from the sweat reach the patch, our yeast receptors will sense their presence, and produce the aforementioned colored pigment corresponding to the level of the IL in question.

The biosensor design (Fig. 1) relies on the dimerization of modified human IL receptors on the yeast plasma membrane upon activation by their respective ligands. The modified receptors contain a synthetic transcription factor bound on their intracellular side that can be released upon dimerization of the receptors. Specifically, dimerization of the receptors leads to the co-localization of two halves of a split-actuator protein, which, when re- united, leads to cleavage of the protein resulting in the release of the transcription factor. The synthetic transcription factor, in turn, binds a specific synthetic promoter and finally activates the expression of the colorimetric reporter gene.

Our vision is a modular design, allowing for easy swap of the interleukin receptor in the yeast, so the patch can be tailored to the need of the individual patient group. The yeast is powered by designed receptors that boast very high sensitivity and selectivity, coupled with an efficient pheromone pathway for signal amplification. The SPY can be photographed with a specialized app to analyze the exact level of coloration regardless of light conditions.




Partnership Timeline

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  1. Zola H. Analysis of receptors for cytokines and growth factors in human disease. Dis Markers. 1996;12(4):225-240. doi:10.1155/1996/807021 ↩
  2. Gunde T, Barberis A. Yeast growth selection system for detecting activity and inhibition of dimerization-dependent receptor tyrosine kinase. Biotechniques. 2005;39(4):541-549. doi:10.2144/000112011↩

About us

Comprising this team are nine ambitious students from various walks of life, who have come together to produce CIDosis - a non-invasive device that can help people with chronic inflammatory diseases visualize their level of inflammation.

Helpful Links

The Team
Promotional Video
Team Poster
Achievements

Address

University of Copenhagen
Thorvaldsensvej 40, Frederiksberg C
Denmark

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