Difference between revisions of "Team:UCopenhagen"

 
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         <p>Chronic Inflammatory Diseases (CIDs) are a source of great mental and physical distress to whoever suffers from them, and continue to be of great <br>
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cost to society. Living with a CID, your well-being is at the mercy of your flare-ups and remissions – inconsistent and unpredictable good days followed by <br>
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bad days. Sufferers are left needing frequent hospital check-ups, and are thus always reminded of their condition despite not looking sick to others. This <br>
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for their entire lives.</p>
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         <a href="https://2020.igem.org/Team:UCopenhagen">
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           <p class="wahed" style="font-size: 24px; font-family: Avenir, Montserrat; color: #052155">Rubor. Calor. Tumor. Dolor</p>
 +
          <p class="khamse fade" style="font-size: 24px; font-family: Avenir, Montserrat; color: #052155">. <span style="color: #f3565c;">Sensor.</span></p>
 
         </div>
 
         </div>
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        <p>We have designed a sweat collecting patch, that CID patients can use to monitor their inflammation status. Thise patch can be placed on the skin and will change color dependent on the inflammation level. Our self-monitoring patch, CIDosis, is an easy-to-use, non-invasive device suitable for home use, and will decrease the need for frequent hospital visits </p>
 
 
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       <h2 style="text-align: center;">Chronic Inflammatory Diseases</h2><div class="format">
−
        <p> Our designed patch will contain a genetically modified yeast, that will respondt to the presence of inflammatory biomarkers (interleukins). Human interleukin receptors that can bind to the interleukin are cloned into yeast, allowing for precise detection of the biomarker in the sweat. When the receptor binds a signal will be sent through the yeast via the endogenous pheromone pathway, resulting in a final visible color output. </p>
+
      <b>Chronic Inflammatory Diseases (CIDs)</b> are debilitating, leaving your well-being
−
       </div>
+
      at the mercy of your flare-ups and remissions. Due to the complexity of these diseases,
−
       <div class="photo">
+
      no two patients' experiences are the same. Likewise, the right treatment for two people,
−
        <a href="https://2020.igem.org/Team:UCopenhagen">
+
       even if they have the same disease, can be different, and to treat these diseases
−
          <img src="https://static.igem.org/mediawiki/2020/3/3f/T--UCopenhagen--RTK_GIF_V1.gif" width="450">
+
       properly, patients therefore need to <b>monitor the progression of their disease closely</b>
−
        </a>
+
      to gather as much information about it as possible.</div>
−
      </div>
+
 
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                <img src="https://static.igem.org/mediawiki/2020/f/fd/T--UCopenhagen--complete_patch.png">
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              </div>
 +
              <div class="txt">
 +
                <h2>Our Solution</h2><div class="format">
 +
                To supplement current testing methods, we have designed <b>a sweat collecting patch</b>
 +
                that patients with CIDs can use to monitor their inflammation status on the go.
 +
                The plaster will sit on the patient's skin and change color depending on the
 +
                patient's level of inflammation, as it reflects itself in the patient's sweat.
 +
                This'll grant them an easy-to-use, non-invasive device that'll give them
 +
                insight into the progression of their disease, and provide them and their
 +
                healthcare professionals with valuable information about their illness, without
 +
                the discomfort surrounding current, more invasive testing methods.</div>
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <div class="third norm txt">
 +
            <h2>What does this mean for the patients?</h2><div class="format">
 +
            Our tool stands for <b>patient empowerment.</b> Our self-monitoring device is
 +
            suitable for home use, and does not have to interfere with patients' daily
 +
            lives or act as a reminder of their illness. Instead, patients can go about their
 +
            day, knowing that they're collecting data on their disease while performing
 +
            other tasks. <br>
 +
            It allows for transparency for the patient regarding the progression of their CID,
 +
            can aid them in discovering the best self-management strategies that work for them,
 +
            and most of all, the collected data can help better guide healthcare professionals
 +
            in evaluating the efficacy of current or new treatment plans, allowing for faster
 +
            treatment adjustment.
 +
            <br>
 +
            CIDosis brings you the data needed to get <b>a clearer picture of your disease.</b></div>
 +
            <img src="https://static.igem.org/mediawiki/2020/b/b8/T--UCopenhagen--clearer_image.png">
 +
          </div>
 +
          <div class="fourth">
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            <div class="todelt">
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              <div style="max-width: 55%;" class="txt">
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                <h2>The Science</h2> <div class="format">
 +
                Our patch will contain <b>genetically modified yeast</b> expressing <b>engineered interleukin
 +
                receptors.</b> These interleukin receptors will bind and react to the inflammatory biomarkers (interleukins)
 +
                found in sweat, whereby a fused intracellular <b>split protein</b> will be recomplemented.
 +
                The recomplementation of the split protein then triggers a signaling pathway,
 +
                in which our signal is amplified, eventually resulting in the production of a pigment,
 +
                which will act as our reporter.
 +
                <br>
 +
                For more information on our engineered interleukin receptors and biosensor components,
 +
                check out our project design page!</div>
 +
              </div>
 +
              <div style="width: 40%;" class="image">
 +
                <img src="https://static.igem.org/mediawiki/2020/8/82/T--UCopenhagen--design_system_loop.gif">
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <div class="fifth norm txt">
 +
            <div class="txt">
 +
              <h2>Three Tiers of Biosensor Design</h2> <div class="format">
 +
              Our scientific design has gone through many iterations, <b>guided especially
 +
              by our human practices and dry lab findings.</b> Through our engineering work,
 +
              we've come up with three different designs, in which we have three different
 +
              tiers of signal amplification, and three different intracellular modifications
 +
              to our interleukin receptors.</div>
 +
            <img style="width: 65%;" src="https://static.igem.org/mediawiki/2020/3/3f/T--UCopenhagen--dynamic-ranges.png">
 +
              <div class="format">For our design and engineering processes, check out our design page, modeling page, human practices page, and engineering success page!</div></div>
 +
          </div>
 +
          <div class="fun_fact txt">
 +
            <h3 style="font-family: Bavro; font-size: 28px; text-align: center;">Fun Fact!</h3>
 +
            <span style="text-align: center; font-family: Avenir; font-size: 16px;">Inflammation is tied to heat, pain, redness, and swelling. In latin,
 +
            that's <b>calor, dolor, rubor, and tumor.</b> It fits perfectly with developing an
 +
            inflammation <b>biosens-or</b> ;)</span>
 +
         
 +
          </div>
 +
          </div>
 +
    </div>
 +
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Latest revision as of 23:45, 27 October 2020

Description Design Implementation Poster
Engineering Success Modeling/Simulation Parts Overview Results Protocols Notebook Safety
Integrated Human Practices Entrepreneurship Education
Collaborations Partnership Our Contribution
Judging Form Medals
Team Members Attributions Sponsors


Rubor. Calor. Tumor. Dolor

. Sensor.

Chronic Inflammatory Diseases

Chronic Inflammatory Diseases (CIDs) are debilitating, leaving your well-being at the mercy of your flare-ups and remissions. Due to the complexity of these diseases, no two patients' experiences are the same. Likewise, the right treatment for two people, even if they have the same disease, can be different, and to treat these diseases properly, patients therefore need to monitor the progression of their disease closely to gather as much information about it as possible.

Our Solution

To supplement current testing methods, we have designed a sweat collecting patch that patients with CIDs can use to monitor their inflammation status on the go. The plaster will sit on the patient's skin and change color depending on the patient's level of inflammation, as it reflects itself in the patient's sweat. This'll grant them an easy-to-use, non-invasive device that'll give them insight into the progression of their disease, and provide them and their healthcare professionals with valuable information about their illness, without the discomfort surrounding current, more invasive testing methods.

What does this mean for the patients?

Our tool stands for patient empowerment. Our self-monitoring device is suitable for home use, and does not have to interfere with patients' daily lives or act as a reminder of their illness. Instead, patients can go about their day, knowing that they're collecting data on their disease while performing other tasks.
It allows for transparency for the patient regarding the progression of their CID, can aid them in discovering the best self-management strategies that work for them, and most of all, the collected data can help better guide healthcare professionals in evaluating the efficacy of current or new treatment plans, allowing for faster treatment adjustment.
CIDosis brings you the data needed to get a clearer picture of your disease.

The Science

Our patch will contain genetically modified yeast expressing engineered interleukin receptors. These interleukin receptors will bind and react to the inflammatory biomarkers (interleukins) found in sweat, whereby a fused intracellular split protein will be recomplemented. The recomplementation of the split protein then triggers a signaling pathway, in which our signal is amplified, eventually resulting in the production of a pigment, which will act as our reporter.
For more information on our engineered interleukin receptors and biosensor components, check out our project design page!

Three Tiers of Biosensor Design

Our scientific design has gone through many iterations, guided especially by our human practices and dry lab findings. Through our engineering work, we've come up with three different designs, in which we have three different tiers of signal amplification, and three different intracellular modifications to our interleukin receptors.
For our design and engineering processes, check out our design page, modeling page, human practices page, and engineering success page!

Fun Fact!

Inflammation is tied to heat, pain, redness, and swelling. In latin, that's calor, dolor, rubor, and tumor. It fits perfectly with developing an inflammation biosens-or ;)


About us

Comprising this team are nine ambitious students from various walks of life, who have come together to produce CIDosis - a non-invasive device that can help people with chronic inflammatory diseases visualize their level of inflammation.

Helpful Links

The Team
Promotional Video
Poster
Medals

Address

University of Copenhagen
Thorvaldsensvej 40, Frederiksberg C
Denmark

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