Difference between revisions of "Team:Queens Canada"

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                       <h3>An estimated 2 million</h3>
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                       <h3>An estimated 2 million Canadians</h3>
 
                        
 
                        
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                       <h4>Canadians suffer from Chronic Kidney Disease each year.</h4>
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                       <h4>suffer from Chronic Kidney Disease <br>each year.</h4>
 
                       <h5>  
 
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                       QGEM’s biosensor monitors in vivo levels of phosphate, potassium, parathyroid hormone, FGF23 and                                                             
 
                       QGEM’s biosensor monitors in vivo levels of phosphate, potassium, parathyroid hormone, FGF23 and                                                             
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                         <video class = actual-intro-video src="https://static.igem.org/mediawiki/2020/6/6b/T--Queens_Canada--Sample_Home_Video.mp4" type =             video/mp4" controls="true"></video>
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                            <source src = "https://static.igem.org/mediawiki/2020/4/4f/T--Queens_Canada--homepage-video-2.mp4" type = video/mp4>
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                        <a href = https://static.igem.org/mediawiki/2020/e/eb/T--Queens_Canada--Promo-Transcript.pdf target = _blank><button onclick = pauseHomeVideo() class = primary-button id = home-transcript-btn>Transcript</button></a>
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         <h2>How much phosphate is in a...</h2>
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         <h3 style="color: #fff; text-align: center;">So how big of an <span style="color: #dbbadd">issue</span> is this?</h3>
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        <h5 style="color: #fff; text-align: center;">Guess how much phosphate is in your favourite foods <br>Click below to find out...<br><br></h3>
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                             <p>There are 652.2mg of phosphate in a Sausage McGriddle&copy;</p>
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                             <p>There are <b>652.2mg</b> of phosphate in a Sausage McGriddle&copy;</p>
 
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                             <p>There are 652.2mg of phosphate in a Tim Horton's&copy; Muffin</p>
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                             <p>There are <b>203.0 mg</b> of phosphate in a Tim Horton's&copy; Muffin</p>
 
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                             <p>There are 652.2mg of phosphate in a Chicken Teriyaki sandwich from Subway&copy;</p>
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                             <p>There are <b>570.7 mg</b> of phosphate in a Chicken Teriyaki sandwich from Subway&copy;</p>
 
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     <p style="display: block;">This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract. This is where you will put the abstract.</p>
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     <p style="display: block; text-align: left; font-weight: 400; font-size: 1.1em; line-height: 1.6;">Team Queen's Canada will be designing a novel transdermal biosensor for point-of-care diagnostic quantification of phosphate, potassium, parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) for chronic kidney disease (CKD) patients. Knowledge of the concentration of these analytes is critical for guiding effective treatments and improving patient outcomes. We will generate genetically encoded Förster resonance energy transfer (FRET) sensors by fusing the N- and C-termini of phosphate-binding protein (PBP), potassium-binding protein (KBP), PTH 1 receptor (PTH1R), and α-Klotho to fluorescent proteins (FPs). To quantify physiologically relevant levels of phosphate, potassium, PTH and FGF23, highly optimized mNeonGreen and mCherry will be used as the FPs. Site-directed mutagenesis will be employed on protein-fluorophore constructs to add a Cysteine residue, to which a glutamate (E)/ lysine (K) coiled-coil system will be added to immobilize the construct onto a microfluidic surface. Through frequent monitoring and early disease detection, we hope to revolutionize CKD diagnostics.
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Latest revision as of 02:23, 28 October 2020


Meet Velcrion.

QGEM’s biosensor monitors in vivo levels of phosphate, potassium, parathyroid hormone, FGF23 and glucose to better treat CKD patients

An estimated 2 million Canadians

suffer from Chronic Kidney Disease
each year.

QGEM’s biosensor monitors in vivo levels of phosphate, potassium, parathyroid hormone, FGF23 and glucose to better treat CKD patients
particles graphic

Together - high Phosphate, Potassium, Parathyroid Hormone, FGF23 and glucose levels can potentially pose a life-threatening risk to CKD patients.

Frequent monitoring of interstitial fluid in CKD patients can prove to be lifesaving, but in Canada blood is taken only every 6 weeks!

We want to change that.

particles graphic

So how big of an issue is this?

Guess how much phosphate is in your favourite foods
Click below to find out...

Sausage McGriddle©

There are 652.2mg of phosphate in a Sausage McGriddle©

Tim Horton's© Muffin

There are 203.0 mg of phosphate in a Tim Horton's© Muffin

Subway Chicken Teriyaki©

There are 570.7 mg of phosphate in a Chicken Teriyaki sandwich from Subway©

Team Queen's Canada will be designing a novel transdermal biosensor for point-of-care diagnostic quantification of phosphate, potassium, parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) for chronic kidney disease (CKD) patients. Knowledge of the concentration of these analytes is critical for guiding effective treatments and improving patient outcomes. We will generate genetically encoded Förster resonance energy transfer (FRET) sensors by fusing the N- and C-termini of phosphate-binding protein (PBP), potassium-binding protein (KBP), PTH 1 receptor (PTH1R), and α-Klotho to fluorescent proteins (FPs). To quantify physiologically relevant levels of phosphate, potassium, PTH and FGF23, highly optimized mNeonGreen and mCherry will be used as the FPs. Site-directed mutagenesis will be employed on protein-fluorophore constructs to add a Cysteine residue, to which a glutamate (E)/ lysine (K) coiled-coil system will be added to immobilize the construct onto a microfluidic surface. Through frequent monitoring and early disease detection, we hope to revolutionize CKD diagnostics.