Reagents required:

• PCR clean up kit (E.N.Z.A)
• PCR reaction product

Procedure:

1. Transfer 30-50 μL of the PCR product to a new 1.5 mL microcentrifuge tube.
2. Add 4-5 times volume of the CP buffer.
3. Briefly vortex and centrifuge the samples.
4. Insert the Hibind DNA mini columns into the 2 mL collection tubes.
5. Add the samples from step 3 to the Hibind DNA mini column.
6. Centrifuge at 13000 g for 60 seconds at room temperature. Discard the filtrate and re use the collection tubes.
7. Add 700 μL of DNA wash buffer diluted with ethanol to the tubes.
8. Centrifuge at maximum speed for 60 seconds. Discard the filtrate and re use the collection tubes.
9. Repeat step 7 for a second DNA wash buffer step.
10. Centrifuge the empty Hibind DNA columns at maximum speed for 2 minutes to dry the column. This step is critical to remove the trace ethanol.
11. Transfer the Hibind DNA column to a new 1.5 mL microcentrifuge tube.
12. Add 50 μL of the elution buffer directly into the Hibind DNA column.
13. Keep at room temperature for 2 minutes and centrifuge at maximum speed for 60 seconds.
14. Store the DNA at -20.

HTML, aka HyperText Markup Language, is the dominant markup language for creating websites and anything that can be viewed in a web browser.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.