Team:UCopenhagen/Experiments

Description Design Implementation Poster
Engineering Success Modeling/Simulation Parts Overview Results Protocols Notebook Safety
Integrated Human Practices Entrepreneurship Education
Collaborations Partnership Our Contribution
Judging Form Medals
Team Members Attributions Sponsors

PROTOCOLS

PCR Cleanup

Reagents required:

  • PCR clean up kit (E.N.Z.A)
  • PCR reaction product

Procedure:

  1. Transfer 30-50 μL of the PCR product to a new 1.5 mL microcentrifuge tube.
  2. Add 4-5 times volume of the CP buffer.
  3. Briefly vortex and centrifuge the samples.
  4. Insert the Hibind DNA mini columns into the 2 mL collection tubes.
  5. Add the samples from step 3 to the Hibind DNA mini column.
  6. Centrifuge at 13000 g for 60 seconds at room temperature. Discard the filtrate and re use the collection tubes.
  7. Add 700 μL of DNA wash buffer diluted with ethanol to the tubes.
  8. Centrifuge at maximum speed for 60 seconds. Discard the filtrate and re use the collection tubes.
  9. Repeat step 7 for a second DNA wash buffer step.
  10. Centrifuge the empty Hibind DNA columns at maximum speed for 2 minutes to dry the column. This step is critical to remove the trace ethanol.
  11. Transfer the Hibind DNA column to a new 1.5 mL microcentrifuge tube.
  12. Add 50 μL of the elution buffer directly into the Hibind DNA column.
  13. Keep at room temperature for 2 minutes and centrifuge at maximum speed for 60 seconds.
  14. Store the DNA at -20.
What is HTML?
HTML, aka HyperText Markup Language, is the dominant markup language for creating websites and anything that can be viewed in a web browser.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.
What is Web Development?
Web Development broadly refers to the tasks associated with developing functional websites and applications for the Internet. The web development process includes web design, web content development, client-side/server-side scripting and network security configuration, among other tasks.

About us

Comprising this team are nine ambitious students from various walks of life, who have come together to produce CIDosis - a non-invasive device that can help people with chronic inflammatory diseases visualize their level of inflammation.

Helpful Links

The Team
Promotional Video
Team Poster
Achievements

Address

University of Copenhagen
Thorvaldsensvej 40, Frederiksberg C
Denmark

Icons made by Freepik from www.flaticon.com