Team:UCopenhagen/Design
So... what are you doing, exactly?
How nice of you to ask! Our aim is to ease the lives of people with chronic inflammatory diseases by providing them with an easy way to monitor their inflammation level. For this, we’ve developed a yeast-based biosensor located within a patch that’ll sit on the patient’s skin, where it’ll continuously gather sweat to analyze. If the yeast detects the presence of a certain interleukin in the sweat, it’ll start a signaling cascade ultimately resulting in the production of a pigment, which will reflect the amount of interleukins found in the sweat.
Venturing deeper into this page will show you how we hope to achieve this by developing our very own receptor-systems!
Interleukin Receptors
Most interleukin receptors fall under the category of Receptor Tyrosine Kinases (RTKs), and thus rely on the dimerization of at least one primary receptor and an accessory receptor (MAYBE WRONG SOURCE https://pubmed.ncbi.nlm.nih.gov/8718782/ ). As similar RTKs aren’t normally found in S. Cerevisiae (here they mention that it’s a null background environment https://pubmed.ncbi.nlm.nih.gov/16235566/ ), we hypothesize that the intracellular signal transduction native to the RTKs will be hard to replicate in our yeast cells. Instead, we’ll attempt to design our own interleukin receptors, while taking advantage of the natural affinity the two extracellular portions of the human interleukin receptors have for each other.
This year, we’re simultaneously looking into three different interleukin receptors, each with their own unique properties.
IL-1
IL-1
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IL-6
Lots of good things here
IL-10
Oh nono da da
Three Tiers of Biosensor Designs
Intracellularly we have 3 designs because that's what made sense to us :) There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don't look even slightly believable. If you are going to use a passage of Lorem Ipsum, you need to be sure there isn't anything embarrassing hidden in the middle of text. All the Lorem Ipsum generators on the Internet tend to repeat predefined chunks as necessary, making this the first true generator on the Internet. It uses a dictionary of over 200 Latin words, combined with a handful of model sentence structures, to generate Lorem Ipsum which looks reasonable. The generated Lorem Ipsum is therefore always free from repetition, injected humour, or non-characteristic words etc.
Ease Pease
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.
Contrary to popular belief, Lorem Ipsum is not simply random text. It has roots in a piece of classical Latin literature from 45 BC, making it over 2000 years old. Richard McClintock, a Latin professor at Hampden-Sydney College in Virginia, looked up one of the more obscure Latin words, consectetur, from a Lorem Ipsum passage, and going through the cites of the word in classical literature, discovered the undoubtable source. Lorem Ipsum comes from sections 1.10.32 and 1.10.33 of "de Finibus Bonorum et Malorum" (The Extremes of Good and Evil) by Cicero, written in 45 BC. This book is a treatise on the theory of ethics, very popular during the Renaissance. The first line of Lorem Ipsum, "Lorem ipsum dolor sit amet..", comes from a line in section 1.10.32.
Bruh
Lots of good things here
Heyyyy
Oh nono da da