Team:UCopenhagen/Experiments

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Protocol 1

Reagents required:

Competent cells
Vector containing DNA

Procedure:

Add 1% volume of the vector to competent cells.
Mix by gently flicking the tube. Vortex will prevent transformation.
Incubate the cells on ice for 30-60 minutes.
Water bath at 42℃ for 45 seconds.
Again incubate on ice for 3-5 minutes.
Add 950 μL of LB and place them in shaking incubator at 37℃ for 1 hour.
Centrifuge at 13000 RPM for 1 minute.
Discard the supernatant slowly, leaving behind 50 μL-100 μL in the tube.
Resuspend the pellet using a pipette and spread on to an appropriate selection plate and incubate overnight at 37℃.

Protocol 2

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Protocol 3

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Protocol 4

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Protocol 5

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Protocol 6

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Protocol 7

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Protocol 8

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Protocol 9

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Protocol 10

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Protocol 11

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Protocol 12

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Protocol 13

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Protocol 14

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Protocol 15

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Protocol 16

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Protocol 17

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Protocol 18

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Protocol 19

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Protocol 20

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Protocol 21

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