Overview
With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+), where T7 promoter and lac operator would be induced with IPTG. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control. Our parts are as follows:
Basic Parts
| Part Number | Description | Length (bp) |
|---|---|---|
| BBa_R0187 | T7 promoter (lac Irepressible) | 44 |
| BBa_K2549058 | HA tag | 27 |
| BBa_K3183024 | HA Tag for L.reuteri | 27 |
| BBa_K2382008 | 6X His Tag | 18 |
| BBa_M10045 | Myc Tag | 30 |
| BBa_K3648001 | C-type lectin domain family 5 member A | 483 |
| BBa_K3648002 | Envelope protien | 1485 |
| BBa_K3648005 | TRS-110*3 | 102 |
| BBa_K3648006 | TRS-151*8 | 243 |
| BBa_K3648007 | TRS-110R*7 | 255 |
| BBa_K3648008 | S-tag | 45 |
| BBa_K3648009 | Thrombin site | 16 |
| BBa_K3648010 | T7 terminator | 48 |
| BBa_K3648014 | TRS-151*7 | 213 |
Composite Parts
| Part Number | Description | Length (bp) |
|---|---|---|
| BBa_K3648003 | pET-29b(+)_CLEC5A | 655 |
| BBa_K3648004 | pET-29a(+)_E protein | 1687 |
| BBa_K3648011 | pET-29b(+)_TRS-110*3 | 313 |
| BBa_K3648012 | pET-29b(+)_TRS-110R*7 | 466 |
| BBa_K3648013 | pET-29b(+)_TRS-151*8 | 414 |
| BBa_K3648015 | pET-29b(+)_TRS-151*7 | 384 |
