The prototype is mainly divided into three parts. The left half consists of a camera and a glass plate, which are responsible for controlling and detecting the movement of the droplets; the right half is a circuit board, which provides the LED light and external power interface; Besides, the middle is separated by the camera bracket. The shell design starts from the specific location of each component. (1)For the large chip on the bottom board, it needs to be fixed to prevent it from moving; (2)For the left half of the camera, glass plate, and chip part, the left and right double door structure is designed to open enough space for the chip, then the chip can be removed and washed. Hinges and magnets are designed to fit the components tightly; (3)For the right half of the circuit board, a trapezoidal structure is designed to save space. At the same time, we leave space for USB interface and LED lighting, and punch holes on the side to facilitate the access and observation of external power Instrument operation, wiring, etc. The overall design is magnificent and beautiful and fits well with the project connotation.
We are honored to introduce our hardware part, in which we have some cool components and designs.
First of all, the electrochemical workstation which plays the role of signal conversion and transmission in E-CRISPR is introduced. This micro electrochemical analysis system named MINOX 2020 was independently developed by Intelligent
Perception and Robotics Research Group from East China University of Science and Technology. It can be conveniently applied to portable electrochemical detection, on-site real-time diagnosis, on-line environmental monitoring, micro-total
analysis system and other fields. It has many advantages, such as USB DC power supply, automatic signal calibration, multi-function electrode sensor interface, cross-platform operation software, real-time signal acquisition and visualization,
and integrated signal analysis and processing program. Please enjoy its charm in the video below.
Finally, we’d glad to introduce our work, 3D printing design of D-E-tector shell. This shell was completed by our team member Zhang Liangkun under the guidance of Dr Gu Zhen. Next, we will show each part and whole of this model separately.
First of all, from the overall perspective, the middle part is the bracket of the prototype, the circuit board and the glass plate part of the fixed microfluidic control. Next, we will show the prototype part first.
This is the prototype stand.
(1)The bottom plate is a circuit board;
(2)A bracket with a certain height in the middle is used to fix the lens;
(3)On the left is a fixing plate for fixing the microfluidic glass plate.
These parts will be shown below.
A fixed plate fixed the microfluidic glass plate.
(1)Viewed from the top, a lock structure is designed to make the fixing more secure;
(2)From the bottom, there is a small round hole, which is the light inlet part. This and the fixed lens on the top constitute a system for easy monitoring.
This part is designed for the shell. It mainly includes two doors that can be opened to the left and right (one of which is a perspective effect), the front shell and the lower floor.
Because the right part of the prototype is a circuit board, the height of the components is low, so only the USB interface, LED power light display port and line interface are needed. The internal design is hollow structure, and the upper part is designed with a label slot.
These are the two doors on the left. When replacing the microfluidic glass plate, it can be opened to both sides. The magnetic structure is designed to make the connection between the two parts firmer.
Finally, considering that the circuit board at the bottom cannot be exposed, the bottom board must be designed to fix the lower part of the prototype. We design screw interface for easy installation. The more ingenious thing is that the USB interface and the hole for the LED power light are combined by the bottom plate and the front door.
The following is a physical display picture.