Team:GreatBay SCIE/Attributions
Table of Attributions
The following table shows the attributions of our team members. ranked from 0 to 5 (mushrooms):
Weighted Attribution Score
Details
Wetlab
Aptamer Experiments
For the SELEX of beta-amatoxin aptamers, rounds 1-11 were done by Katherine, and rounds 12-15 were done by Jacky.
For the verification of binding of aptamers to the toxins, Katherine, Chloe, Max, Winnie and Jacky carried out ELONA assay for verifying alpha and beta aptamers' binding ability, and Katherine, Chloe, Jacky carried out Dot Blot experiments.
scFv Polypeptide Experiments
Roger, Ginny, and Alex form the scFv production team of our project. They were involved in the production of this polypeptide using Escherichia coli.
Roger, Ginny, and Alex carried out multiple ELISA assays to verify the binding specificity of scFv antibodies.
Roger, Lambert, Ginny, Winnie and Alex are the workforce in all kinds of scFv purification and painful debugging.
Hardware Experiments
Katherine performed the conjugation of gold particles with reporter probe and aptamer, which is examined by absorbance peak shift.
Katherine and Chloe performed in the toxin-test line immobilization on NC membrane which verifies the concept of a test line and proves the feasibility of Au nanoparticle.
Katherine and Chloe incorporate the model designed by Katherine to the determination of the most sensitive concentration of aptamer for the detection in hardware, with the optimized thermal cycle number.
Wiki Construction
Max built the overall structure and coded the major parts of the HTML and CSS codes. Winnie, Jerry, Jacky, and other teammates helped upload some pages and files. Many of us gave valuable opinions on the website layout and design.
Modeling
Immobilization pad position model was completed by Max.
Disassociation constant model was completed by Katherine.
Human Practices
Public Engagement and Education Outreach
Alex, Ginny, and Alice went to Shenzhen Youth Activity Centre to teach primary school children about electrophoresis, and other experiments as well as knowledge in Biology. Winnie was responsible for contacting and organization.
Alex and Jerry are the SCIE iGEM ECA leaders, and many of us participated in the operation of the ECA: for example, Mandy, Jerry, and Alex prepared contents for our ECA courses.
Winnie, Ginny, Evangeline, and Alex went up on stage and did the TEDx SCIE speech to spread knowledge of Synthetic Biology. Winnie was responsible for contacting and organization.
Ginny, Winnie and Alex gave out presentation of our project to the attendees of Bluepha Synbio Summer Camp.
Integrated Human Practices
Winnie, Ginny, Alice, Regina, Adonie, Max and Roger traveled to Guangzhou for visiting the Guangdong Institute of Microbiology and interviewed Professor Deng, and for climbing the Baiyun Mountain to collect mushroom samples.
Chloe and Katherine designed the questionnaires to investigate the potential users and the public's opinions on our product.
Chloe and Katherine contacted and arranged Zoom meetings with Dr. Candace Bever, Dr. Ciara O'Sullivan, Dr. Miriam Jauset-Rubio, and Dr.Strzałka, the rest of the team also attended the meetings.
Ginny contacted and arranged a Zoom meeting with EPFL iGEM 2019, and Ginny, Chloe, Katherine, Adonie, Alex, Lambert, and Jerry participated in the meeting.
Evangeline contacted and interviewed the emergency department doctor, Dr. Ying Yang.
Jacky and Ginny have written the script and recorded our CCiC presentation, Winnie, Mandy, and Evangeline created our CCiC poster.
Chloe chatted with the potential users of our product online and obtained great suggestions and feedback.
Art, Design, Photography, Video Editing and Music
Winnie was responsible for designing the whole wiki page and illustration(including that cute loading animation on the wiki) as well as designing gifts for people who helped the team.
Winnie was the director of our Promotion Video.
Ginny, Winnie, and Jerry have written the lyrics of the song in our Promotion Video, and Max, Ginny, Winnie, Katherine, and Chloe helped in composing and recording the song.
Lambert edited all of our videos.
Winnie and Alice have drawn artworks we used in the presentations of our project (e.g. Wiki, promotion video, presentation posters, presentation PowerPoint background et cetera.)
Max designed the team logo, the ShroomSweeper logo, and some of the website elements used in our Wiki.