Life is defined by three core biomolecules: DNA, RNA and proteins, where proteins take the spotlight in synthetic biology. In contrast, we, the iGEM Team Heidelberg aim to engineer RNA to enable specific interactions between all three biomolecule chemistries.
To see how, take a look at our Project Description and follow along on our journey...
True to our motto – A Link Between Proteins – we use our RBPs and RNA linkers to construct specific protein-RNA interactions.
From assembling split GFP, to recruiting protein domains to RNAs of interest.
For complete control over protein-RNA interactions, we want to go a step further and dynamically modify RNA inside the living cell. Towards this goal, we engineered versatile split variants of the Tetrahymena thermophila Group I intron.
Having visited protein-RNA and RNA-RNA interactions, we move on to the final boss – sequence-specific dsDNA-RNA interactions. We design RNA-controllable promoters using dsDNA-RNA triple helices and triple helix forming motifs.
An iGEM Team is not an island and science cannot be seen in isolation from society. Therefore we chose to engage with the public by designing an experimental course based on our project and organising a science slam to bring the message of iGEM to the masses. At every step of our project we engaged with out stakeholders to see where we could go. Take a look at our Human Practices page to get an inside view of our journey.
All of this would not have been possible without our wonderful team members, advisors, instructors and the one and only Professor Wölfl, as well as our sponsors and the numerous people who helped us out along the way.