Team:Heidelberg/Materials

Materials
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Table 1: Antibiotics.

Antibiotic Company
Ampicillin AG Wölfl, Heidelberg, Germany
Chloramphenicol Honeywell Fluka, Charlotte, USA
Kanamycin Carl Roth, Karlsruhe, Germany
Tetracycline Carl Roth, Karlsruhe, Germany

Table 2: Enzymes.

Enzyme Company
BsaI V2 New England Biolabs, Ipswich, USA
EspI3 New England Biolabs, Ipswich, USA
Hi-T4 DNA Ligase New England Biolabs, Ipswich, USA
High-Fidelity 2X PCR Master Mix New England Biolabs, Ipswich, USA
T4 DNA Ligase New England Biolabs, Ipswich, USA
Taq 2X Master Mix New England Biolabs, Ipswich, USA

We ordered our Primers and DNA Sequences with the generous sponsoring of IDT and Twist Biosciences. Check out our project overview page to find our Level 0 cloning sequences. Or just find them here.

Table 3: Materials.

Material Company
Agarose Carl Roth, Karlsruhe, Germany
Bacto-Tryptone BD Biosciences, Franklin Lakes, USA
Bacto-Yeast Extract Carl Roth, Karlsruhe, Germany
CaCl2 * 2 H2O Merck, Darmstadt, Germany
DFHBI Merck, Darmstadt, Germany
DirectLoad Wide Range DNA Marker Merck, Darmstadt, Germany
DMSO Genaxxon, Ulm, Germany
DPBS Gibco Life Technologies, Carlsbad, USA
E. coli Top 10 strain AG Niopek, Heidelberg, Germany
Ehidium bromide Carl Roth, Karlsruhe, Germany
Entry Vector René Inckeman and the iGEM Team Marburg 2018
Ethanol Merck, Darmstadt, Germany
Glucose Carl Roth, Karlsruhe, Germany
Glycerol Acros Organics, Fair Lawn, USA
KCl Merck, Darmstadt, Germany
Marburg Collection René Inckeman and the iGEM Team Marburg 2018
MgCl2 * 6 H2O Carl Roth, Karlsruhe, Germany
MgSO4 * 6 H2O Carl Roth, Karlsruhe, Germany
NaCl Merck, Darmstadt, Germany
Purple loading dye New England Biolabs, Ipswich, USA
T4 DNA Ligase buffer New England Biolabs, Ipswich, USA
TAE buffer Luis Hauptmann, Heidelberg, Germany

Table 4: Kits.

Kit Company
PureYield Plasmid Miniprep System Promega, Madison, USA
NEB Monarch Gel Extraction Kit New England Biolabs, Ipswich, USA

Table 5: Consumables.

Consumable Company
0.22 µm filters Merck, Darmstadt, Germany
Nunc 96-well plate black flat Thermofisher, Waltham, USA

Table 6: Equipment.

Equipment Company
Allegra X-12R Centrifuge Beckman Coulter, Brea, USA
GloMax Promega, Madison, USA
Microfuge® 18 Centrifuge Beckman Coulter, Brea, USA
ProFlex PCR System thermocycler Thermofisher, Waltham, USA
Transluminator Vilber Lourmat, Eberhardzell, Germany
HT Multitron Incubator INFORS, Basel, Switzerland