Team:Heidelberg/Materials
Materials
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Table 1: Antibiotics.
| Antibiotic | Company |
|---|---|
| Ampicillin | AG Wölfl, Heidelberg, Germany |
| Chloramphenicol | Honeywell Fluka, Charlotte, USA |
| Kanamycin | Carl Roth, Karlsruhe, Germany |
| Tetracycline | Carl Roth, Karlsruhe, Germany |
Table 2: Enzymes.
| Enzyme | Company |
|---|---|
| BsaI V2 | New England Biolabs, Ipswich, USA |
| EspI3 | New England Biolabs, Ipswich, USA |
| Hi-T4 DNA Ligase | New England Biolabs, Ipswich, USA |
| High-Fidelity 2X PCR Master Mix | New England Biolabs, Ipswich, USA |
| T4 DNA Ligase | New England Biolabs, Ipswich, USA |
| Taq 2X Master Mix | New England Biolabs, Ipswich, USA |
We ordered our Primers and DNA Sequences with the generous sponsoring of IDT and Twist Biosciences. Check out our project overview page to find our Level 0 cloning sequences. Or just find them here.
Table 3: Materials.
| Material | Company |
|---|---|
| Agarose | Carl Roth, Karlsruhe, Germany |
| Bacto-Tryptone | BD Biosciences, Franklin Lakes, USA |
| Bacto-Yeast Extract | Carl Roth, Karlsruhe, Germany |
| CaCl2 * 2 H2O | Merck, Darmstadt, Germany |
| DFHBI | Merck, Darmstadt, Germany |
| DirectLoad Wide Range DNA Marker | Merck, Darmstadt, Germany |
| DMSO | Genaxxon, Ulm, Germany |
| DPBS | Gibco Life Technologies, Carlsbad, USA |
| E. coli Top 10 strain | AG Niopek, Heidelberg, Germany |
| Ehidium bromide | Carl Roth, Karlsruhe, Germany |
| Entry Vector | René Inckeman and the iGEM Team Marburg 2018 |
| Ethanol | Merck, Darmstadt, Germany |
| Glucose | Carl Roth, Karlsruhe, Germany |
| Glycerol | Acros Organics, Fair Lawn, USA |
| KCl | Merck, Darmstadt, Germany |
| Marburg Collection | René Inckeman and the iGEM Team Marburg 2018 |
| MgCl2 * 6 H2O | Carl Roth, Karlsruhe, Germany |
| MgSO4 * 6 H2O | Carl Roth, Karlsruhe, Germany |
| NaCl | Merck, Darmstadt, Germany |
| Purple loading dye | New England Biolabs, Ipswich, USA |
| T4 DNA Ligase buffer | New England Biolabs, Ipswich, USA |
| TAE buffer | Luis Hauptmann, Heidelberg, Germany |
Table 4: Kits.
| Kit | Company |
|---|---|
| PureYield Plasmid Miniprep System | Promega, Madison, USA |
| NEB Monarch Gel Extraction Kit | New England Biolabs, Ipswich, USA |
Table 5: Consumables.
| Consumable | Company |
|---|---|
| 0.22 µm filters | Merck, Darmstadt, Germany |
| Nunc 96-well plate black flat | Thermofisher, Waltham, USA |
Table 6: Equipment.
| Equipment | Company |
|---|---|
| Allegra X-12R Centrifuge | Beckman Coulter, Brea, USA |
| GloMax | Promega, Madison, USA |
| Microfuge® 18 Centrifuge | Beckman Coulter, Brea, USA |
| ProFlex PCR System thermocycler | Thermofisher, Waltham, USA |
| Transluminator | Vilber Lourmat, Eberhardzell, Germany |
| HT Multitron Incubator | INFORS, Basel, Switzerland |