Team:KUAS Korea/Results
Results
Introduction
We couldn't get into our lab this year. What we did can be divided into two sections. First, we modeled and predicted behaviors of systems in our final product. Second, we designed our future experiments.
Patch prediction model
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Original Sequences in the Reference Paper |
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H1 |
GAGCGATACTGTTGGTAGATGTCGCCATGTCTTGCGACATCTACCAACAGCGAGACAACAGAGAGACGGTCGGGTCCACAGTTTCC |
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H2 |
GGAAACTGTGTCGAGTAGAGTGTGGGCTCTCTGTTGTCTCGAGATGTCGCAAGACATGGCGACATCTACCAACAGCCATGTCTTG |
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C |
GACATCTACCAACAGTATCGCTC |
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Modified Sequences with 3 elongated base pairs |
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C1 |
CTGGACATCTACCAACAGTATCGCTC |
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C2 |
TGTGACATCTACCAACAGTATCGCTC |
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C3 |
GTTGACATCTACCAACAGTATCGCTC |
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Modified Sequences with 4 elongated base pairs |
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C4 |
CTGTGACATCTACCAACAGTATCGCTC |
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C5 |
TGTTGACATCTACCAACAGTATCGCTC |
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C6 |
GTTGGACATCTACCAACAGTATCGCTC |
The table above is the sequences we designed. We added 3-4 nucleotides to the original C sequence that are complementary to a region with in itself so that it would bind to itself and form a loop.
We modeled 4 things and got results of them. (1) Heat transfer and distribution (2) the behavior of the system according to temperature (3) the behavior of the system according to time (4) visibility
(1) Heat transfer and distribution
The figure above is the heat distribution we modeled.
(2) The behavior of the system according to temperature
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Modified Sequences with elongated base pairs |
Threshold Temperature (°C) |
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C1 |
CTGGACATCTACCAACAGTATCGCTC |
51 |
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C2 |
TGTGACATCTACCAACAGTATCGCTC |
34 |
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C3 |
GTTGACATCTACCAACAGTATCGCTC |
40 |
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C4 |
CTGTGACATCTACCAACAGTATCGCTC |
34 |
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C5 |
TGTTGACATCTACCAACAGTATCGCTC |
42 |
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C6 |
GTTGGACATCTACCAACAGTATCGCTC |
Not found |
The table above is the threshold temperatures where the patch starts to be activated.
(3) The behavior of the system according to time
It is assumed that it takes about 25 minutes to fully activate the patch system.
(4) visibility
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Name |
Molar concentration (M) |
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H1 |
\( 4.08 \times 10^{-06} \) |
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H2 |
\( 4.08 \times 10^{-06} \) |
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Cs |
\( 4.08 \times 10^{-09} \) |
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DFHBI-1T |
\( 8.15 \times 10^{-05} \) |
The table above is the molar concentrations needed to make the patch visible when it is activated.
Details are described in model section. [Link]
Experiment design
We designed our future experiment plan.
Details are described in experiments section. [Link]