Team:KUAS Korea/Results

Results

Introduction

We couldn't get into our lab this year. What we did can be divided into two sections. First, we modeled and predicted behaviors of systems in our final product. Second, we designed our future experiments.

Patch prediction model

Original Sequences in the Reference Paper

H1

GAGCGATACTGTTGGTAGATGTCGCCATGTCTTGCGACATCTACCAACAGCGAGACAACAGAGAGACGGTCGGGTCCACAGTTTCC

H2

GGAAACTGTGTCGAGTAGAGTGTGGGCTCTCTGTTGTCTCGAGATGTCGCAAGACATGGCGACATCTACCAACAGCCATGTCTTG

C

GACATCTACCAACAGTATCGCTC

Modified Sequences with 3 elongated base pairs

C1

CTGGACATCTACCAACAGTATCGCTC

C2

TGTGACATCTACCAACAGTATCGCTC

C3

GTTGACATCTACCAACAGTATCGCTC

Modified Sequences with 4 elongated base pairs

C4

CTGTGACATCTACCAACAGTATCGCTC

C5

TGTTGACATCTACCAACAGTATCGCTC

C6

GTTGGACATCTACCAACAGTATCGCTC

The table above is the sequences we designed. We added 3-4 nucleotides to the original C sequence that are complementary to a region with in itself so that it would bind to itself and form a loop.

We modeled 4 things and got results of them. (1) Heat transfer and distribution (2) the behavior of the system according to temperature (3) the behavior of the system according to time (4) visibility

(1) Heat transfer and distribution

The figure above is the heat distribution we modeled.

(2) The behavior of the system according to temperature

Modified Sequences with elongated base pairs

Threshold Temperature (°C)

C1

CTGGACATCTACCAACAGTATCGCTC

51

C2

TGTGACATCTACCAACAGTATCGCTC

34

C3

GTTGACATCTACCAACAGTATCGCTC

40

C4

CTGTGACATCTACCAACAGTATCGCTC

34

C5

TGTTGACATCTACCAACAGTATCGCTC

42

C6

GTTGGACATCTACCAACAGTATCGCTC

Not found

The table above is the threshold temperatures where the patch starts to be activated.

(3) The behavior of the system according to time

It is assumed that it takes about 25 minutes to fully activate the patch system.

(4) visibility

Name

Molar concentration (M)

H1

\( 4.08 \times 10^{-06} \)

H2

\( 4.08 \times 10^{-06} \)

Cs

\( 4.08 \times 10^{-09} \)

DFHBI-1T

\( 8.15 \times 10^{-05} \)

The table above is the molar concentrations needed to make the patch visible when it is activated.

Details are described in model section. [Link]

Experiment design

We designed our future experiment plan.

Details are described in experiments section. [Link]