# Results

## Introduction

We couldn't get into our lab this year. What we did can be divided into two sections. First, we modeled and predicted behaviors of systems in our final product. Second, we designed our future experiments.

## Patch prediction model

 Original Sequences in the Reference Paper H1 GAGCGATACTGTTGGTAGATGTCGCCATGTCTTGCGACATCTACCAACAGCGAGACAACAGAGAGACGGTCGGGTCCACAGTTTCC H2 GGAAACTGTGTCGAGTAGAGTGTGGGCTCTCTGTTGTCTCGAGATGTCGCAAGACATGGCGACATCTACCAACAGCCATGTCTTG C GACATCTACCAACAGTATCGCTC Modified Sequences with 3 elongated base pairs C1 CTGGACATCTACCAACAGTATCGCTC C2 TGTGACATCTACCAACAGTATCGCTC C3 GTTGACATCTACCAACAGTATCGCTC Modified Sequences with 4 elongated base pairs C4 CTGTGACATCTACCAACAGTATCGCTC C5 TGTTGACATCTACCAACAGTATCGCTC C6 GTTGGACATCTACCAACAGTATCGCTC

The table above is the sequences we designed. We added 3-4 nucleotides to the original C sequence that are complementary to a region with in itself so that it would bind to itself and form a loop.

We modeled 4 things and got results of them. (1) Heat transfer and distribution (2) the behavior of the system according to temperature (3) the behavior of the system according to time (4) visibility

#### (1) Heat transfer and distribution

The figure above is the heat distribution we modeled.

#### (2) The behavior of the system according to temperature

 Modified Sequences with elongated base pairs Threshold Temperature (°C) C1 CTGGACATCTACCAACAGTATCGCTC 51 C2 TGTGACATCTACCAACAGTATCGCTC 34 C3 GTTGACATCTACCAACAGTATCGCTC 40 C4 CTGTGACATCTACCAACAGTATCGCTC 34 C5 TGTTGACATCTACCAACAGTATCGCTC 42 C6 GTTGGACATCTACCAACAGTATCGCTC Not found

The table above is the threshold temperatures where the patch starts to be activated.

#### (3) The behavior of the system according to time

It is assumed that it takes about 25 minutes to fully activate the patch system.

#### (4) visibility

 Name Molar concentration (M) H1 $$4.08 \times 10^{-06}$$ H2 $$4.08 \times 10^{-06}$$ Cs $$4.08 \times 10^{-09}$$ DFHBI-1T $$8.15 \times 10^{-05}$$

The table above is the molar concentrations needed to make the patch visible when it is activated.

Details are described in model section. [Link]

## Experiment design

We designed our future experiment plan.

Details are described in experiments section. [Link]