Team:Mingdao/Activity

Start

Design, make & play Board Game of Dental Caries

Education
01~30 DEC

Make and promote SynBio animation video

Education
01~31 MAR

Re-transform (pDL278)

Cloning and Transformation
01 APR

Colony PCR for S. mutans & PCR check for Sm and Ss

Cloning and Transformation
10 APR

Hold STEAM Fair in Mingdao High School

Education
13~17 APR

Site-directed mutagenesis of pDL278

Cloning and Transformation
20 APR

Meet up with NCTU_Formosa

Human Practices
24 APR

Visit SEPOM Technology Ltd.

Human Practices
29 APR

Cloning of ldhp-RBS / pSB1C3

Cloning and Transformation
01 MAY

Colony PCR for S. mutans & PCR check for Sm and Ss

Cloning and Transformation
11 MAY

Promote SynBio and recruit iGEM 2021 Members in iGEM Audition Conference in Biolab, Mingdao High School

Education
18~22 MAY

Transformation of S. mutans (colony PCR)

Cloning and Transformation
3 JUN

Cloning of GFP-Tr / pDL278

Cloning and Transformation
8 JUN

Cloning of GFP-Tr / pDL278

Cloning and Transformation

Sm & Ss identification in saliva of team members

Measurements
11 JUN

Sm & Ss antagonism test

Measurements
15 JUN

Sm & Ss antagonism test

Measurements
15 JUN

Hold and participate in Debate Competition for GMO

Education
16 JUN

Hold education activities in Chiao-Jen Elementary School

Education
19 JUN

Cloning of ldhp / pDL278

Cloning and Transformation
22 JUN

Cloning of ldhp-Aqua / pDL278
Cloning of RBS-PO-Tr / pSB1C3

Cloning and Transformation

Sm, Ss & EcN survival rate measurement under different pH value

Measurements
06 JUL

Cloning of Cat-Tr / pSB1C3

Cloning and Transformation
08 JUL

H2O2 production in Ss analysis

Measurements
11 JUL

Sm survival rate measurement under different concentrations of H2O2

Measurements
13 JUL

Meet up with NCKU_Tainan

Measurements

Visit Genmont Biotechnology Inc.

Human Practices
15 JUL

Meet Dentistry Department of China Medical University

Human Practices
17 JUL

Cloning of ldhp-GFP-Tr / pDL278

Cloning and Transformation
21 JUL

Cloning of ldhp-Aqua / pDL278
Cloning of tpxp-GFP-Tr / pDL278

Cloning and Transformation
27 JUL

Ldhp / tpxp promoter activity assay

Measurements
29 JUL

Meet Chung Shan Medical University Oral Medicine Department

Human Practices
08 AUG

Interview with patients and doctors in Dental Clinic

Human Practices
08~15 AUG

Attend Taiwan iGEM Conference in NCHU, Taichung

Human Practices
17~18 AUG

Cloning of tpxp-Cat-Tr / pSB1C3

Cloning and Transformation
25 AUG

Cloning of ldhp-Aqua-PO-Tr-tpxp-Cat-Tr / pSB1C3

Cloning and Transformation
29 AUG

Present preliminary achievement in Annual iGEM Intramural Presentation in Mingdao High School

Education
04 SEP

Cloning of ldhp-Aqua-tpxp-GFP-Tr / pSB1C3
Cloning of ldhp-Aqua-PO-Tr-tpxp-GFP-Tr / pSB1C3

Cloning and Transformation
08 SEP

AQP functional assay

Measurements
13 SEP

Collaborate with CCU_Taiwan

Human Practices
13~14 SEP

SpxB functional assay

Measurements
15 SEP

KatG functional assay

Measurements
20 SEP

AQP with KatG functional assay

Measurements
23 SEP

Protein expression of AQP, SpxB, and KatG by SDS-PAGE analysis

Measurements
29 SEP

Wild-type EcN or GM EcN against Sm antagonism competition test

Measurements
01 OCT

H2O2 production assay in our prototype, Cleandy

Measurements
10 OCT
End