Team:Mingdao/Attributions

    This year, we have accomplished an amazing work, although we still need to improve the prototype and further complete the final “probiotic” of S. mutans, which loses the ability to produce lactic acid and gains the function of H2O2 production to antagonize the wild type.

    We are close to achieve our goal because of a lot of help between team members, collaborations & partnership, as well as experts, biotech companies, sponsors and others who were engaging and participating in our project. Here, through the contents of Attributions, we showed our strongest appreciation to them.


TEAM MEMBERS


  Team leader

    Our team is composed of 30 members from Senior High Department and International Department at Mingdao High School. We have two team leaders from both of the departments to hold meetings, organize activities and coordinate lab works.

  • JUI-HUNG YEH of Senior High Department
  • YU-LIN WU of International Department


    •   Project design


        Brainstorming

  • All team members were engaging in ideas, researches and discussion in our project.


    •     Experiment design

  • CHU-HSUAN SHEN, TING-YUN LIAO and YI-HSUAN YU plotted the blueprint and the schematic diagram to clearly demonstrate our project design.
  • CHU-HSUAN SHEN, SHENG-FONG LI and CHIANG-YU CHEN worked with Lab PI of Dr. PEI-HONG CHEN to prepare experimental materials, design primers, order or request bacteria, etc.


    •   Wet lab


        Gene cloning

  • SHENG-FONG LI is the leader in gene cloning. He’s well trained in skills of molecular biology since he started lab internship at Grade 8. He taught other members to accomplish all the works of gene cloning process.

  • CHIANG-YU CHEN, CHU-HSUAN SHEN, BO-CHE WU, YU-ZE HUANG, KAI-YUAN TU, CHENG-WEI LIN and JIE-NI LIU are the members who were always doing and completing the gene cloning in the lab. They performed restriction enzyme digestion, PCR, plasmid extraction, gel electrophoresis, primer design, etc. They also helped team CSUM_Taiwan to complete many gene cloning for their toehold switch sensors.

  • Other members sometimes help to conduct routine experiments such as restriction enzyme digestion, plasmid extraction and gel electrophoresis.


    •     Measurement & functional assay

  • CHIANG-YU CHEN & CHU-HSUAN SHEN were working together and spending a lot of time studying/writing the protocols and conducting the experiments including antagonism test, growth inhibition by H2O2, H2O2 concentration measurement, etc.


    •     Manipulate Biosafety Level 2 bacteria in P2 lab in Chung Shan Medical University (CSMU)

  • CHIANG-YU CHEN, CHU-HSUAN SHEN, CHENG-WEI LIN and JIE-NI LIU had special training for doing Biosafety Level 2 bacteria. They were always working in a class II biological safety cabinet under the supervision of a collegiate iGEM team, CSMU_Taiwan and Prof. Ming-Shiou Jan at Chung Shan Medical University.


    •   Dry lab


        Modeling

  • YI-KAI LIAO, CHIH-FANG CHEN and JUI-HUNG YEH worked together in reviewing papers, measuring parameters in bacterial growth experiments, coding and building models. They collaborated with team CCU_Taiwan and went to the CCU_Taiwan’s lab to perform metabolite analysis with Bruker Ascend 400 MHz NMR spectrometer in National Chung Cheng University.


    •     Hardware / software

  • YI-SYUN LIAO, SHAO-YUAN TIEN, TAI JAN TSAO and SHIH-SHIUAN PENG had an idea to create an anaerobic culture incubator. However, they currently failed to build one. Nevertheless, they did put lots of efforts on it and taught us how to better culture S. mutans in an anaerobic environment.


    •   Enterprise


        Product R&D

  • ZI-XUAN CHEN & WAN-HUA CHANG met experts, visited biotech company, researched candies on market, made homemade lozenges for our team project.


    •     Marketing

  • KUAN-YU HUANG, YU-LIN WU, PIN-HSUN LIN, ZI-YI KUNG, JIE-NI LIU, HSIN-HSIN WU put hard efforts on survey, market research, SWOT analysis, product package design, website building, and related works.


    •   HP


        Integrated human practices

  • YA-ZHU JHAN is the leader in integrated human practices. She organized and coordinated all the related works.

  • CHIH-FANG CHEN, Ting-Yung Liao, Yi-Hsuan Yu, Wan-Hua Chang, Zi-Xuan Chen, Chun-Jui Tien and Yung-Hsiang Yang were working together on visiting experts, biotech companies, medical schools in the universities, as well as doing street surveys and meeting up with Taiwan’s iGEM teams.

  • All team members were engaging in human practice and education activities.


    •     Education

  • CHIH-FANG CHEN is the leader in education. She organized and coordinated all the related works.

  • SHU-HAN LIU, ZI-YI KUNG, HSIN-HSIN WU, YU-LIN WU and KUANG-YU LO were working together on holding STEAM fair activities, GMO debate, workshops in an elementary school and intramural presentation for our achievement.

  • All team members were engaging in human practice and education activities.


    •     Science communication

  • KUANG-YU LO, YU-LIN WU and PIN-HSUN LIN designed a board game based on our concept of project. Through the game, the participants can learn what causes tooth decays and how to prevent them.


    •     Sustainable Development Goal (SDGs)

  • TING-YUN LIAO & YI-HSUAN YU were engaging in SDGs activities in Mingdao High School and presented our project in SDGs’ community.


    •   Art

  • PEI-TZU CHEN, HSIN-HSIN WU and ZI-YI KUNG are responsible for all the art and design works in our project concerning logo, posters, product, banner, animation and others.


    •   Wiki


        Coding and design

  • YUNG-HSIANG YANG is responsible for our wiki design and editing including coding in HTML, CSS, and JavaScript.


    •     Content

    The wiki contents are huge tasks, which were accomplished by our team work. The below table listed the members who were in charge of the work.


      Video


        Promotion video

  • Video director: TING-YUN LIAO
  • Animation creators: CHIH-FANG CHEN, YI-HSUAN YU
  • Cast: CHU-HSUAN SHEN, YUNG-HSIANG YANG, SHENG-FONG LI, JUI-HUNG YEH, CHIANG-YU CHEN
  • Voice: CHU-HSUAN SHEN


    •     Presentation video

  • Video director: CHU-HSUAN SHEN
  • Film editors: TING-YUN LIAO, CHIH-FANG CHEN, YI-HSUAN YU
  • Cast: CHU-HSUAN SHEN, ZI-XUAN CHEN, YI-KAI LIAO, CHIANG-YU CHEN, YU-LIN WU, KUANG-YU LO, KUAN-YU HUANG
  • Cameo appearance: students in Mingdao High School


    • INSTRUCTORS

        Dr. PHIL (PEI-HONG) CHEN
    Dr. Chen has led the iGEM teams of Mingdao since 2014 till now. He taught us experimental skills in the molecular biology laboratory, and instructed us on both cloning technique and experiments. Our team preparation thus became more comprehensive. Contact: phil@ms.mingdao.edu.tw

        Mrs. CHIA-LE MENG
    She has taught us how to do some of the experiments, including gel electrophoresis, DNA extraction, etc. She also helped us solve problems in terms of human practice and wiki editing. Contact: chialemeng@ms.mingdao.edu.tw


    EXPERTS AND ADVISORS

        Dr. CHUN-CHENG CHEN
    Dr. Chen, who works at College of Oral Medicine, Chung Shan Medical University, gave us notices about the mechanisms of our experiment.

        MEDICAL COLLEGE STUDENTS
    The students in the Department of Dentistry at China Medical University School suggested us about the mechanisms as well as the combination between our project and clinical demand.

        Dr. WEN-YING SHEN
    Dr. Shen of HiNew Dental Clinic/高新牙醫診所 gave us clinical advices about our final product.

        Dr. MING-TA LI
    Dr. Li of Guang Qun Dental Clinic/光群牙醫診所 advised us about the product in clinical trials.

        Dr. CHIEN-FAN LI
    Dr. Li of Shine Dental Clinic/晶采牙醫診所 gave us suggestions about how our product can work.

        Dr. MING-SHIOU JAN
    Dr. Jan of Chung Shan Medical University provided us with Streptococcus sanguinis and instructed us some experiment related to it. He helped and supervised us in manipulating this BioSafety Level 2 strain in his lab.

        Dr. Yuqing Li
    Dr. Li of Sichuan University in China gave Streptococcus mutans and taught us how to culture the bacteria in an anaerobic condition and engineer the genome of S. mutans by CRISPR/Cas9 system.

        Dr. Eugene Lin
    Dr. Lin of National Chung Cheng University helped us design the experiments and perform bacterial metabolite analysis using Nuclear Magnetic Resonance Spectrometer (NMR).


    SPONSORS

        Mingdao High School
    Mingdao High School built up a biolab based on synthetic biology, where we're doing iGEM with equipment for molecular biology research. It also provided funding for the iGEM competition.

        GenScript Biotech
    We got fundings and materials from them including qPCR probes for detecting 5 different kinds of oral bacterial strains.

        GenMont Biotech Incorporation
    They gave us suggestions to form our final product, as well as some comments about probiotics and basic regulations of food safety in the Taiwan’s market.

        Sepoms Technology CO., LTD.
    They provided us with fundings and gave us advices about the relationship between oxidative stress and caries.

        HiNew Dental Clinic/ 高新牙醫診所
    They provided us with fundings and gave us clinical advices about our final product.

        Guang Qun Dental Clinic/ 光群牙醫診所
    They provided us with fundings and advised us about the product in clinical trials.

        Shine Dental Clinic/ 晶采牙醫診所
    They provided us with fundings and gave us suggestions about how our product can work.