Team:Mingdao/Collaborations
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Mingdao
This year we’ve collaborated with several iGEM collegiate and high school teams in Taiwan. We’ve developed a H2O2-generating device in a probiotic E. coli Nissle, and they helped us in bacterial culture, product design, and modeling and shared the valuable experiences with us.
On the other hand, based on our laboratory setup, we utilized Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments) to help team CCU_Taiwan analyze the fluorescent spectra for a chemical dye used in their project.
For partnership, we did collaborate with team CSMU_Taiwan throughout this year based on each team’s resource, experimental skills and iGEM experiences. We did gene cloning, GFP signal testing and glucose analysis for them. And they helped us collect saliva samples, culture a Biosafety Level 2 bacterium and taught how to adjust media at different pH values. For further information, go to see our PARTNERSHIP
On the other hand, based on our laboratory setup, we utilized Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments) to help team CCU_Taiwan analyze the fluorescent spectra for a chemical dye used in their project.
For partnership, we did collaborate with team CSMU_Taiwan throughout this year based on each team’s resource, experimental skills and iGEM experiences. We did gene cloning, GFP signal testing and glucose analysis for them. And they helped us collect saliva samples, culture a Biosafety Level 2 bacterium and taught how to adjust media at different pH values. For further information, go to see our PARTNERSHIP
IN OUR PROJECT
Bacterial culture
Team CSMU_Taiwan helped us culture S. sanguinis at Biosafety Level 2 in a class II biological safety cabinet. They taught us how to manipulate the bacteria correctly in a P2 laboratory. We worked on it under the supervision of experts and all the experiment will be done in Chung Shan Medical University with them and supervised under the guidance of Prof. Jan.
Project design
Team TAS_Taipei inspired us the way to make a probiotic into a product. They provided us a probiotic strain, E. coli Nissle, which was applied in their previous iGEM project in 2018. They also guided us the protocol for transforming the bacteria, as well as shared the experiences from the public feedback and iGEM judges’ comments.
Modeling
Team CCU_Taiwan is specialized in bacterial metabolite analysis. They used a NTD$10-million Bruker Ascend 400 MHz NMR spectrometer to perform a lot of chemical compound investigation. They helped us examine the metabolites in S. mutans grown in various kinds of glucose, and taught us how to build a mathematical modeling to simulate pH variation.
IN CCU_TAIWAN PROJECT
We used Synergy H1 Hybrid Multi-Mode Microplate Reader to examine a fluorescent chemical property for team CCU_Taiwan. They developed a glass fiber sensor to detect viruses with the fluorescent dye of 4-Aminothiophenol, which was conjugated to a viral specific binding peptide. We helped perform emission fluorescence spectra scan to determine the emission spectrum range for the biosensor setting.
