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Team:Montpellier/Contribution

Team:Montpellier - 2020.igem.org

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Team:Montpellier

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CONTRIBUTION



This page aims to explain the team contribution to iGEM parts for the bronze medal criteria.
In order to achieve our parts contribution, we decided to act on two fronts.
First, we constructed parts linked to Phagent that we wanted to work on for the wetlab. Those parts have been registered to the iGEM parts catalog. On one hand, there are some basic parts:



Table 1: Basic Parts
Number of the part Description Length
BBa_K3700000 Spacer in order to clear sequence and make generic primers 40
BBa_K3700001 Spacer in order to clear sequence and make generic primers 40
BBa_K3700002 Spacer in order to clear sequence and make generic primers 40
BBa_K3700003 Spacer in order to clear sequence and make generic primers 40
BBa_K3700005 Secretion signal to export protein out of the cell 60

Then they were used to build composite parts, combined with other preexisting parts of the catalog:



Table 2: Composite Parts
Name Design Description
BBa_K3700004 sfGFP linked to the dacA gene with an inducible promoter surrounded by spacers in order to create universal primers.
DacA helps the bacteria to activate the STING pathway by producing di-AMPc5. We will add a GFP to find the bacteria containing correct clones, and to assess protein production easily.
BBa_K3700006 sfGFP linked to the dacA gene with a constitutive promoter surrounded by spacers in order to create universal primers.
DacA helps the bacteria to activate the STING pathway by producing di-AMPc5. We will add a GFP to find the bacteria containing correct clones, and to assess protein production easily.
BBa_K3700007 sfGFP with a secretion signal and an inducible promoter surrounded by spacers in order to create universal primers.
sfGFP with a secretion signal in order to check if the cell can secrete proteins.
BBa_K3700008 sfGFP with a secretion signal and a constitutive promoter surrounded by spacers in order to create universal primers.
sfGFP with a secretion signal in order to check if the cell can secrete proteins.
BBa_K3700009 sfGFP linked to the Azurin gene with a secretion signal and an inducible promoter surrounded by spacers in order to create universal primers.
Verification of the expression and the secretion of the azurin thanks to the sfGFP.
BBa_K3700010 sfGFP linked to the Azurin gene with a secretion signal and a constitutive promoter surrounded by spacers in order to create universal primers.
Verification of the expression and the secretion of the azurin thanks to the sfGFP.
BBa_K3700011 VHH-PDL1 with a secretion signal and an inducible promoter surrounded by spacers in order to create universal primers.
BBa_K3700012 VHH-PD-L1 with a secretion signal and a constitutive promoter surrounded by spacers in order to create universal primers.

Secondly, as we worked on M13 phage but did not build parts directly related to it, we decided to contribute to iGEM registry by adding information on M13 parts registered by other teams previously.
This concerns BBa_K2255008, which code for two domains of an M13 coat protein; these domains play an important role in the infection process. The second coding sequence on which we added a little information is BBa_M13008 which codes for another coat protein.