Team:NEFU China/Notebook

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 Nov.

 Oct.

 Sep.

 Aug.

 Jul.

 Jun.

 May.

 Jan.

 19.Dec.

 19.Nov.

Nov.

9

2020.11.9 We uploaded the team poster.

Oct.

1

2020.10.1 Successfully loaded the bacteria in agarose and alginate gels and induced luminescence.

2

2020.9.20-2020.10.2 We built the hardware measuring device and upgraded it.

5

2020.10.5 We knocked out yhaK and constructed the last plasmid.

10

2020.8.10-2020.10.10 We conduct joint experiments with the QHFZ_China Team.

12

2020.10.12 We tested the threshold of the yqjF promoter after addition of the YhaJ transcription factor.

15

2020.10.15 We finished all the mathematical modeling.

17

2020.10.17 Successful random mutation of YhaJ and validation of the effect.

18

2020.10.18 We had a roadshow.

29

2020.10.29 We uploaded the team presentation video.

Sep.

2

2020.9.2 We started to mutate the yqjF promoter.

24

2020.9.24 We uploaded project promotion video.

25

2020.9.25 Successful mutation of the 3rd generation yqjF promoter. As the same time, we designed our uniforms.

Aug.

2

2020.8.2 We interviewed retired officer online, as the same time, we consructed the first plasmid.

5

2020.8.5 We attended the exchange meeting with QHFZ_China Team.

7

2020.8.7 We constructed the key plasmid for phage-assisted directed evolution, MP.

10

2020.8.10 A lecture was held in Tianjin Xianshuigu No.1 High School to let students share the ideas about iGEM and learned related knowledge about Synthetic Biology. As the same time, We provided plasmids for QHFZ_China Team.

16

2020.8.16 A lecture was held for pupils to let students learn more about biology and cultivate interest in biology. We also tested the mutation rate of the MP plasmid using a rifampin resistance assay.

18

2020.8.18 We attended the exchange meeting with BUCT Team.

24

2020.8.24 We constructed engineered bacteria to emit Lux bioluminescence.

25

2020.8.15-2020.8.25 We have experimentally validated the yqjF promoter and riboswitch effects and tested the 1st generation yqjF promoter threshold.

27

2020.8.27 We attended the exchange meeting with TJUSLS_China.

31

2020.8.27-2020.8.31 We attended the 7th CCiC Communication Conference, where we heard from the judges.

Jul.

18

2020.7.18 We attended the iGEM Jinlin University Meetup to communicate with other teams.

21

2020.7.21 First day in the lab.

2020.7.21 A lecture was held in Harbin No.6 Middle School to let students share the ideas about iGEM and learned related knowledge about Synthetic Biology.

2020.7.21 We attended the exchange meeting with FAFU-China Team.

24

2020.7.24 We started to do phage assisted directed evolution experiment.

31

2020.7.31 We attended the exchange meeting with NEU_China Team.

Jun.

13

2020.6.13 We used an internet-based questionnaire.

21

2020.6.21 We began preparing our Wiki.

28

2020.6.28 We bought the relevant equipment, bacterial strains and viral strains for directed evolution.

May.

22

2020.5.22 Bio-optical Landmine Detection was determined as the final case and we eliminated some of the team members.

26

2020.5.26 Team registration.

Jan.

14

2020.1.3-2020.1.14 We learned excellent cases in the previous years and related knowledge. After that, we eliminated some of the players according to the results of the examination.

27

2020.1.15-2020.1.27 Brainstorm for the team project.

19.Dec.

14

2019.12.14 Setting up the 2020 NEFU_China iGEM team through interviews.

21

2019.12.21 We held the first meeting, the mobilization meeting, and released the next plan.

19.Nov.

26

2019.11.26 We hold the summary report meeting of 2019 NEFU_China and the recruitment meeting of 2020 NEFU_China.