Team:NJU-China

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E x o s o m e B o m b

S i R N A

Overview

siRNAs offer an opportunity to specifically target mRNAs and modulate the expression of therapeutic targets which can facilitate cancer treatment. Here, we designed composable and programmable genetic circuits that used the liver as a tissue chassis to direct the self-assembly of exogenous siRNAs into secretory exosomes. The circuits include core parts which expressed siRNAs that not only inhibit the cancer cells, but also remove the disguise cancer use to evade the immune system; enhancing parts which facilitated the production of more anti-cancer exosomses, and guidance parts which directed the exosome to target cancer cells. Taken together, the genetic parts assembled into anti-tumor exosomes, secreted into circulation, delivered to cancer cells and eliminate them. The strategy provided a controllable, efficient and convenient cancer treatment which may even provide a personalized treatment strategy that can address a broad range of problems in biomedicine.

It was a peaceful day on the
human body
Bikini Bottom beach . Everything went fine, until
protooncogene
Mr. Krabs was arrested by the
immune cell
police because of
microbe
Plankton ’s slander of poisoning, though he didn’t plead guilty.
protooncogene
Mr. Krabs , who was hurt by all the criticize and misunderstanding, totally changed. He determined to get money as much as possible by all devious means. He made an ultimate goal: making the
cancer cell
Krusty Krab dominate the Bikini Bottom. HP

Firstly,
protooncogene
Mr. Krabs added puppy secretly to his Krabby patties to win more profits. In order to satisfy increasingly hamburger-addicted customers, Mr. Krabs decided to pull his secret magic switch “KRAS” on, making the
cancer cell
Krusty Krab begin endless automatic division. Then, Mr. Krabs commanded his employee
other genes
Squidward to protect the illegally expanding
cancer cell
Krusty Krab from being recognizing by the
immune cell
police .
other genes
Squidward made confusing bubble spray “PD-L1” and “don’t eat me” signal light “CD47” imitations to tell the
immune cell
police that they are law-abiding. Parts

However,
erythrocyte
Squirrel Sandy , discovered that the
protooncogene
Krusty Krab was in unregulated extension, harming the
human body
Bikini Bottom . Worried for no help, Sandy went on the
in vitro
moon and turn to aliens. Our iGem team NJU China 2020 members, as aliens noted
erythrocyte
Sandy ’s recourse, came up a way together to help
human body
Bikini Bottom to defeat Mr. Krabs. We enveloped our
plasmid
plan draft into a
adenovirus
drift bottle and threw it to the
human body
Bikini Bottom . Model

The
adenovirus
drift bottle dropped into Sponge bob’s house, the
liver cell
big pineapple . Sponge bob opened the bottle and got the
plasmid
plan draft to defeat
protooncogene
Mr. Krabs , but he still needs some friends’ help. He called his
expression products
friends together into
liver cell
big pineapple , and then they set out by
exosome
bus . After Pearl, the driver of the
exosome
bus , targeted and opened the door of Krusty Krab,
PD-L1 siRNA
snail Gary used his snail mucus to make the “PD-L1” bubble break,
CD 47 siRNA
Patrick Star took down the “CD47” signal light from the roof of restaurant, and finally,
KRAS siRNA
SpongeBob turned off the switch “KRAS”. Now, the restaurant was under the supervise of
immune cell
police and unable to expand anymore. Soon, the
cancer cell
Krusty Krab was discovered and dismantled by the police, who also arrested Mr. Krabs. The
human body
Bikini Bottom was restored to its former peace. Projects