Team:NJU-China/Parts
Part Overview of NJU-China
This year, NJU-China designed 7 basic parts, 2 composite parts, and compared the original 2 parts from the
perspective of promoting exosome release.
Due to the slow progress of the experiment, some parts were not verified at the experimental level. We chose the
two-phase plan and will verify the effect of all components later. The results of our current experiments can
guarantee the relevant medals.
| Basic Parts | ||||
|---|---|---|---|---|
| Number | Name | Type | Introduction | Medal |
| BBa_K1633008 | nSMase 2 | Coding | nSMase 2 is a key regulatory enzyme generating ceramide from sphingomyelin and can actively induce exosome secretion from cells and trigger cellular export of small RNAs. | Bronze |
| BBa_K2796028 | Exosome Booster | Coding | TExosome booster refers to hSDC4-T2A-STEAP3-P2A-NadB. STEAP3 (involved in exosomebiogenesis), syndecan-4(SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008) | Bronze |
| BBa_K3335000 | KIBRA | Coding | TKIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.( Lin Song et al., 2019) | Gold |
| BBa_K3335003 | iRGD-Lamp2b-siRNA(KRAS) | Coding | We developed an strategy to silence the so-called untargetable and undruggable KRAS gene by employing exosome-mediated siRNA delivery.At the same time, we used iRGD-Lamp2b to achieve exosome targeting. Particularly, we reprogrammed cells to simultaneously express KRAS siRNA and Lamp2b. Lamp2b is an exosomal membrane protein, in fusion with a tumor-penetrating internalizing RGD (iRGD) peptide (CRGDKGPDC), and then produce the tumor-targeting exosomes as KRAS siRNA delivery system. | Gold |
| BBa_K3335004 | SiRNA(PD-L1) | Coding | Tumor cells evade immune system surveillance by overexpressing PD-L1. Therefore, we combined the fusion expression of iRGD and Lamp2b to characterize the exosome targeting.At the same time, siRNA targeting PD-L1 is produced to reduce mRNA expression.This allows the immune system to avoid the effects of PD-L1 and attack tumor cells. | Silver |
| BBa_K3335005 | SiRNA(CD47) | Coding | CD-47 acts as a don't eat me signal to macrophages of the immune system. Both activation and loss of CD47 can result in enhanced proliferation. And CD47 ligation leads to cell death in many normal and tumor cell lines via apoptosis or autophagy. SiRNA was used to reduce the expression of CD47 in tumor cells, allowing tumor cells to be engulfed by macrophages. | Silver |
| BBa_K3335007 | Albumin Promoter | Promoter | Albumin promoter has the liver tissue specific transcriptional activity. The expression specificity of the modified albumin promoter in mice can be guaranteed.It is strongly expressed only in mammalian hepatocytes and not in other cells.This ensures that when we use liver cells as a concept, they are essentially unexpressed in other tissue cells. Enhance overall system security. | Silver |
| BBa_K3335008 | rtTA-tTS | Coding | The rtTA protein is capable of binding the operator only if bound by a tetracycline and activate the expression of TRE-controlled genes in a Tet-On system. Thus the introduction of doxycycline to the system initiates the transcription of the genetic product. On the contrast tetracycline-dependent transcriptional silencer (tTS) binds the tetO inducible promoter in the absence of Dox, thus expression of TRE-controlled genes can be repressed. | Silver |
| BBa_K3335009 | TRE Operator mini CMV | operator | The entirety of several TetO sequences with a minimal promoter is called a tetracycline response element (TRE). | Silver |
| Composite Parts | ||||
| Number | Name | Type | Introduction | Medal |
| BBa_K3335006 | iRGD-Lamp2b-siRNA(P+C+K) | Coding | This part is intend for expression of targeted iRGD peptide and degradation of KRAS PD-L1 and CD47 mRNA in tumor cells. | Gold |
| BBa_K3335010 | pAlbumin-rtTA-tTS-TRE-miniCMV-iRGD-Lamp2b-siRNA(P+C+K) | Coding | The albumin promoter ensures that gene sequences are expressed only in liver cells.At the same time, rtTA and tTs Parts guarantee that the downstream gene will only begin to be expressed when DOX enters.Downstream gene expression IRGD-LAMP2B completes exosome targeting and simultaneously expresses siRNA targeting CD47, KRAS and PD-L1. | Gold |