Team:SZU-China/Parts

SZU-China team has added quantitative experimental characterization data to two existing parts from the Registry of Standard Biological Parts and documented the experimental characterization on the Part's Main Page on the Registry.

You can click on the part number to see the details.

Part Number	Name	Part Description
BBa_K1131000	FMO	This Flavin-containing monooxygenase (FMO) from M. aminisulfidivorans can be expressed in many strains of E. Coli to produce indigo dye.
BBa_K3264022	TALEsp2-tnaA-Fmo	(FMO) from M. aminisulfidivorans can be expressed in many strains of Escherichia coli to produce indigo dye. In the presence of indole and oxygen, FMO can catalyze the addition of a hydroxyl group to indole generating the intermediate indoxyl. Indoxyl then naturally oxidizes to generate indigo.

Basic Parts

This year, we submitted two new parts for the silver medal. You can click on the part number to see the details.

Part Number	Name	Part Description
BBa_K3360003	IARI-SP-2	Encoding the IARI-SP-2 endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST tag.
BBa_K3360004	CL34	Encoding the CL34 endo-beta-1,4-glucanase of Streptomyces.

Composite Parts

The two kinds of basic parts described above and the improved basic part are assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.

Part Number	Name	Part Description
BBa_K3360005	tac-rbs-IARI-SP-2-T	IARI-SP-2 endo-beta-1,4-glucanase of Bacillus subtilis expression system
BBa_K3360010	rbs-CL34-T	CL34 endo-beta-1,4-glucanase of Streptomyces expression system
BBa_K3360012	tac-rbs-bglA-T	bglA expression system

Improved Parts

This year, our team has improved the existing parts. The improved parts (in BLUE) and original existing parts (in GREY) are presented below. You can click on the part number to see the details.

Part Number	Name	Part Description	Improvement
BBa_K3360002 BBa_K1131000	bgla	Encoding the β-glucosidase A of Thermotoga maritima	the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.