Team:TU Kaiserslautern/Experiments

Experiments

If you are interested in replicating or integrating one of our experiments into your own project, having a guide is key. Below you will find our protocols. They are collected as PDF-files by experimental workflow. You will also find record of our time in lab and the experimental results collected in our Labjournals.

• Buffer and Media
• Agarose Gel
• MoClo
• Heat-Shock Transformation
• Macherey-Nagel NucleoSpin Plasmid EasyPure
• Diagnostic Restriction Digest
• Transformation Chlamydomonas reinhardtii
• Cell Harvest and Cell Disruption for Chlorophyll Determination
• Various Lysis Methods
• Screening of Secreting Chlamydomonas reinhardtii
• SDS-Page
• Western Blot and Ponceau-S Staining
• ECL Detection
• Bradford Assay
• TCA Precipitation

• ABTS Assay Protocol
• HPLC Protocol

MoClo LabJournal 03/17/2020 - 09/24/2020 Screening LabJournal 05/29/2020 - 06/18/2020 LabJournal 06/25/2020 - 09/08/2020 Rest LabJournal 03/09/2020 - 10/20/2020	LabJournal 09/01/2020 - 09/10/2020 LabJournal 09/01/2020 - 09/10/2020 LabJournal 08/05/2020 - 08/25/2020 LabJournal 10/12/2020 - 10/14/2020 LabJournal 09/15/2020 - 09/24/2020 LabJournal 10/15/2020 - 10/22/2020 LabJournal 07/21/2020 - 07/29/2020 LabJournal 08/26/2020 - 08/28/2020 LabJournal 09/28/2020 - 10/08/2020 LabJournal 07/30/2020 - 08/04/2020	LabJournal 05/25/2020 - 08/04/2020 LabJournal 08/06/2020 - 10/21/2020