| Date | Content |
|---|---|
| 05/25/2020 - 05/29/2020 | First steps: Glycerine stocks and plasmid |
| 06/02/2020 - 06/04/2020 | Preparation growth test BL21(DE21) marLac and BaLac with/without CuSO4 |
| 06/05/2020 - 06/18/2020 | Test expression BL21(DE21) marLac and BaLac with/without CuSO4 |
| 06/22/2020 - 07/01/2020 | Test expression BL21(DE21) marLac and BaLac with CuSO4 LB-medium vs. 2YT-medium |
| 07/07/2020 - 07/20/2020 | Protein production BL21(DE21) marLac and BaLac with CuSO4 and 2YT-medium |
| 07/21/2020 - 07/29/2020 | Protein production BL21(DE21) marLac with CuSO4 and 2YT-medium |
| 07/30/2020 - 08/04/2020 | Protein production BL21(DE21) BaLac with CuSO4 and 2YT-medium, with/without protease inhibitor |
| 08/05/2020 - 08/25/2020 | Protein production BL21(DE21) BaLac and marLac with CuSO4 and 2YT-medium |
| 08/26/2020 - 08/27/2020 | Cell production BL21(DE21) BaLac with CuSO4 and 2YT-medium, freeze the cell-pellet |
| 09/01/2020 - 09/10/2020 | Protein production BL21(DE21) BaLac with CuSO4 and 2YT-medium |
| 09/15/2020 - 09/22/2020 | Test expression BaLac: BL21(DE21), Codon Plus RIL, Roosetta gami, AD494(DE3), Origami, DH5α; marLac: BL21(DE21), all with CuSO4 and 2YT-medium |
| 09/24/2020 - 10/08/2020 | Protein production BaLac: Rosetta gami, AD494(DE3); marLac: BL21(DE21), all with CuSO4 and 2YT-medium |
| 10/12/2020 - 10/14/2020 | Protein production BL21(DE21) BaLac with the cell-pellet from the 08/27/2020 |
| 10/14/2020 – 10/22/2020 | Protein production AD494(DE3) BaLac with CuSO4 and 2YT-medium and IPTG test with the tac promotor |
Team:TU Kaiserslautern/Notebook
Notebook
Time flies by so quickly and it's not until later one realizes how much was accomplished. On this page you will find a series of calendars detailing the journey of our experiments, collaborations, meetings, and other events. We are proud of the work achieved despite the global restrictions facing us in 2020, and hope we can inspire other groups to be creative with their resources when faced with obstacles.
| Date | Content |
|---|---|
| 05/25/20 | Made Buffers |
| 05/26/20 | Buffer Dilutions |
| 05/27/20 | ABTS abs spectra with T. veriscolor at different pH's |
| 06/02/20 | T. veriscolor Positive Control ABTS Assay |
| 06/03/20 | T. veriscolor Positive Control ABTS Assay |
| 06/08/20 | T. veriscolor Positive Control ABTS Assay |
| 06/15/20 | Preparation of Solutions |
| 06/16/20 | Chlamy ABTS Assay cancelled due to miscommunication |
| 06/18/20 | Chlammy Lysate ABTS Assay |
| 06/22/20 | T. veriscolor Positive Control ABTS Assay |
| 06/23/20 | Prepare new stocks, T. veriscolor Positive Control ABTS Assay |
| 06/29/20 | T. veriscolor Positive Control ABTS Assay |
| 07/01/20 | Drop Assay, Lysate ABTS Assay, Bradford |
| 07/08/20 | T. veriscolor Positive Control ABTS Assay |
| 07/16/20 | E. coli marLac and BaLac BL21 ABTS Assay |
| 07/28/20 | E. coli marLac ABTS Assay |
| 07/29/20 | Diclofenac Preparation, PreHPLC Spectra |
| 08/04/20 | E. coli BaLac BL21 ABTS Assay |
| 08/06/20 | E. coli BaLac BL21 Freezing ABTS Assay |
| 08/13/20 | T. veriscolor Positive Control ABTS Assay, Diclofenac Incubation Spectra |
| 08/19/20 | E. coli BaLac BL21 ABTS Assay |
| 08/24/20 | E. coli BaLac BL21 Freezing ABTS Assay |
| 08/26/20 | E. coli marLac ABTS Assay |
| 08/31/20 | T. veriscolor Positive Control ABTS Assay |
| 09/03/20 | Prepare new stocks, T. veriscolor Positive Control ABTS Assay |
| 09/07/20 | Prepare new stocks, T. veriscolor Positive Control ABTS Assay |
| 09/08/20 | T. veriscolor Positive Control ABTS Assay |
| 09/09/20 | E. coli BaLac BL21 ABTS Assay |
| 09/28/20 | Chlamy Lysate BaLac ABTS Assay |
| 10/05/20 | Chlamy Lysate BaLac ABTS Assay |
| 10/07/20 | E. coli marLac, BaLac AD494, and BaLac Rosetta ABTS Assay |
| 10/12/20 | Chlamy Lysate BaLac ABTS Assay |
| 10/14/20 | HPLC with T. veriscolor and E. coli BaLac BL21 |
| 10/15/20 | HPLC, Chlamy Lysate BaLac Cu Comparison ABTS Assay |
| 10/21/20 | E. coli BaLac AD494 ABTS Assay, HPLC Spectra |
Collaboration Meetings
| Date | Event |
|---|---|
| 3/27/2020 | Central European Meeting with Ambassador Carolina |
| 3/28/2020 | Central European Meeting with Ambassador Carolina |
| 3/29/2020 | Central European Meeting with Ambassador Carolina |
| 3/30/2020 | Central European Meeting with Ambassador Carolina |
| 3/31/2020 | Central European Meeting with Ambassador Carolina |
| 4/9/2020 | Central European Meeting with Ambassador Carolina |
| 4/26/2020 | Central European Meeting with Ambassador Carolina |
| 4/27/2020 | Central European Meeting with Ambassador Carolina |
| 4/28/2020 | Central European Meeting with Ambassador Carolina |
| 5/7/2020 | International Wastewater Group Meeting |
| 5/25/2020 | DKS: First Meeting between Stuttgart and Kaiserslautern |
| 6/2/2020 | DKS: Planning Meetings |
| 6/8/2020 | International Wastewater Group Meeting |
| 6/20/2020 | DKS: First Meeting between Darmstadt and Kaiserslautern |
| 7/4/2020 | Marburg Meetup |
| 7/5/2020 | Marburg Meetup |
| 7/6/2020 | DKS: Planning Meetings |
| 7/9/2020 | International Wastewater Group Meeting |
| 7/31/2020 | DKS: Livestream with Darmstadt |
| 8/6/2020 | DKS: Wiki Meeting |
| 8/13/2020 | International Wastewater Group Meeting |
| 8/20/2020 | DKS: Wiki Meeting |
| 8/24/2020 | German Synthetic Biology Conference |
| 8/25/2020 | German Synthetic Biology Conference |
| 9/3/2020 | DKS: Wiki Meeting |
| 9/21/2020 | DKS: Wiki Meeting |
| 9/22/2020 | Information Meeting for Igem 2021 |
| 9/23/2020 | DKS: Expert Exchange Meeting between Darmstadt and Stuttgart |
| 9/24/2020 | International Wastewater Group Meeting |
| 10/1/2020 | DKS: Wiki Meeting |
| 10/8/2020 | DKS: Podcast with Stuttgart |
| 10/9/2020 | DKS: Wiki Meeting |
| 10/13/2020 | DKS: Wiki Meeting |
| 10/16/2020 | DKS: Wiki Meeting |
| 10/23/2020 | International Wastewater Group Meeting |
Human Practices
| Date | Event |
|---|---|
| 5/21/2020 | Interview with expert Dr.-Ing. Henning Knerr |
| 5/30/2020 | Livestream 1 |
| 6/1/2020 | Interview with expert Mark Benecke |
| 6/6/2020 | iJet Collaboration |
| 7/8/2020 | International Postcard Exchange |
| 7/10/2020 | Interview with expert Dr. Gerd Maack |
| 7/13/2020 | Interview with expert Dr. Ulrich Ehlers |
| 7/15/2020 | Academic Journal for Team Maastricht |
| 7/17/2020 | Livestream 2 |
| 7/30/2020 | Interview with expert Prof. Dr. Antonio Pierik |
| 7/31/2020 | Surveys and Livestream 3 with Darmstadt |
| 8/5/2020 | American Water and Wastewater Treatment Plant tours |
| 8/18/2020 | Discussions with Students in US |
| 9/2/2020 | German Wastewater Treatment Plant tour |
| 9/9/2020 | Interview with expert Dr. Dietmar Schlosser |
| 9/19/2020 | World Cleanup Day |
| 9/23/2020 | Interview with expert Dr. Sabine Sané |
| 10/6/2020 | Day in the Life of a member of Team Kaiserslautern sponsored by Promega |
| 10/7/2020 | Logo Collaboration |
| 10/13/2020 | Interview with experts at the State Authority |
| 10/20/2020 | Muggle Journal for Team Maastricht |
