Team:UNILausanne/Attributions

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Attributions

In our attributions page, we would like to thank all people who helped our team and made this project possible. We are extremely grateful for their support, as well as the experience that each one of them brought to build the project.

show Team members

• Ilinca Dragan was our team captain. She actively managed the general team organization as well as most of the planning for the SwissGEM meetup. In the lab, she participated in the azurin purification process and the kill switch design. Ilinca was also involved as an active member of the Transcriptome blog, in collaboration with the Chalmers-Gothenburg iGEM team.

• Nicolas Pellaton was our vice captain, he helped Ilinca with the team organization. He participated in the repressilator design and experiments and took charge of the MTT assay experiment.

• Thierry Marti worked on the plasmid design and assembly. He designed and developed the kill switch system. He also took part in the azurin expression and purification experiment. He developed and performed the azurin immunodetection assays, and participated in the MTT assay.

• Christopher Forbes-Jaeger participated in the development and testing of the repressilator system and was involved in the data analysis of the experiments. He also worked on the video editing of the iGEM promotional video and on our France collaboration video. He also designed the final logo for the team.

• Jérémy Cherbuin was in charge of preparing all of the competent cells that we used for cloning and testing the repressilator. He also helped in the development and the design of the kill switch.

• Sara Ezzat helped to design and test our various assemblies required in the project. She also wrote for the Transcriptome blog.

• Elise Eray participated in the development and testing of the repressilator system and managed the data analysis. She was also involved in the Transcriptome blog.

• Kuba Korzeniowski was in charge of the wiki design and development. He also participated in the Transcriptome website development. He represented the UNILausanne team in the France collaboration video development.

• Daniel Rodriguez participated actively in the Human Practices part of the project. He got in contact with various people to help us with our project, developed the GMO survey and created the Pro Femme video project.

• Johanna Wahn was the treasurer of the team. She handled our resources and dealt with our distributors and sponsors. She also took part in the modelling part of the project. Finally, she was actively involved in Human Practices, where she helped to develop the Pro Femme video and created the Integrated Human Practices sub-project. She also developed the business plan based on our project idea.

• Pablo Guridi Fernández was in charge of the modeling part of the project. He was in contact with multiple experts to improve the model and worked in collaboration with the wet lab team to make the models as accurate as possible.

• Julien Cergneux initially helped in the modelling project. At the end of the project, he participated in multiple experiment, specially involved for the azurin purification, and the kill switch construction and testing.

showGeneral support

We would like to thank all people that closely helped us during the whole project. Without their advice and their support, the project would not have been possible.

• Prof. Dr. Yolanda Schaerli was the main PI of our project. She was the one who initiated the creation of the first iGEM team of the University of Lausanne (UNIL). She brought a lot of help and support to the team and helped us develop the project. She also secured all the funding that we received and provided access to her laboratory during the autumn semester.

• Prof. Dr. Jan-Willem Veening was our secondary PI on our project. He brought his advice to develop the project and participated in the brainstorming sessions.

showLab support

During our wet lab work, a lot of people were present to guide us and bring some advice. We would then like to thank them for all of the support and the help that they brought us during the whole summer.

Main advisors

• Dr. Javier Santos Moreno and Içvara Barbier helped us with the repressilator experiment design, manipulations and the data analysis. They guided us during the brainstorming and the development of the project.

• Dimitra Synefiaridou helped us with the design of the primers for our various assemblies and taught us how to perform the Golden Gate and Gibson assembly.

• Uyen Linh Ho, as a previous member of the Bielefeld-CeBiTec iGEM team (2015), brought expertise to our new team and informed us about the essential parts of the iGEM competition. She provided support for planning the project and lab manipulation in general, helped with cell culture work and provided us with HAP1 cells and materials.

• Dr. Sudip Das has lent us his expertise on the human microbiota and cancer cells culture. He also helped us with the MTT assay, with the design of the experiment and gave us access to the needed materials.

Additional support

• Vincent de Bakker was a member of the Leiden iGEM team (2016) and an advisor of the Leiden iGEM team (2018). His experience with iGEM allowed us to be as informed as possible about the competition in general.

• Dr. Michael Taschner and Dr. Roberto Vazquez Nunez helped us with the design and azurin expression and purification. They also helped us on azurin immunodetection constructs. They brought their expertise on protein purification, helped us design the experiments and gave us access to their materials and instruments.

• Philippe L'hôte from the Scientific Services of the CIG provided us with HeLa cells necessary for the test of our final product.

• Prof. Dr. Philipp Engel gave us access to the E.coli Nissle 1917 ∆clb strain that we used during the entirety of our project.

• Dr. Manfredo Quadroni and Séverine Lorrain from the Protein analysis facility helped us with the mass spectrometry analysis and imumunodetection of our azurin samples.

• All of the plasmids that we used in our experiment were obtained and ordered from Addgene. They were made available by Prof. Dr. Johan Paulson (plasmids 85460, 85524, 85525, 85526, 85527, 85528, 85529 and 127855) and Prof. Dr. Chris Voigt (plasmid 49377).

showProject advisor

• Dr. John Allan was the mentor of our team. He brought expertise on iGEM and on the project in general. He was an important support for the team and helped us to adapt the project during the summer. He also gave us advice for the wiki and the presentation of the giant Jamboree. We would like to thank him for his constant support and his availability to answer all of our questions.

showSponsors

Without the support of our various sponsors, our iGEM project would never have seen the light of day. We would therefore like to thank them for their trust and their support.

• The Leenaards Fondation was our main sponsor for this project. Without them, it would have been impossible for us to have access to participate at iGEM.

• Promega and New England BioLabs (NEB) sponsored us by offering access to free products. Microsynth and IDT also sponsored us by offering free sequencing and free primers respectively.

• The School of Biology and Marco Zini gave us access to the teaching lab that we used during the summer and the equipment required for the experiments. The School of Biology also gave us the possibility to get ECTS credits for our iGEM participation.

• The Department of Fundamental Microbiology (DMF) of UNIL allowed us to get the glassware cleaned and to autoclaved the waste. They also prepared and sponsored the plates, buffers and media that we used in our experiments. The secretariat of the DMF also helped us with the accounting of the team and receiving the parcels

showHuman Practices support

We would like to thank all people that helped us for our various human practice’s projects. Next to our main project, we decided to explore various domains, and their guidance brought us a huge help.

Main project feedback

• Dr. Anna Wagner is an oncologist at the CHUV, in Lausanne. She gave us a specialist point of view on our project and helped us to understand the classical treatment involved in colorectal cancer.

• Prof. Dr. Laurent Potvin-Trottier is the author of the paper “Synchronous long term oscillations in a synthetic gene circuit” (Potvin-Trottier, L., Lord, N., Vinnicombe, G. et al., Nature 538, 514–517, 2016), which presents the repressilator on which we based our project. He gave us expert advice on the repressilator system and some feedback on our project. He also helped us to understand more deeply the modeling part of their study.

Probiotics feedback

• Redbiotech and Pharmabiome are companies that gave us some advice and feedback on probiotics and on their general production, requirement and application. They allowed us to understand what is already present in the market and how to adapt our project for it.

GMO survey feedback

• Prof. Dr. Marc Audétat and Prof. Dr. Marc Robinson-Rechavi gave us advice and feedback on the public opinion of GMO and gave us feedbacks on our online survey on this subject.

Pro femme video feedback

• Prof. Dr. Christine Sempoux, Annaëlle Morf, Marie Pasquier and Marion Brechet informed us on gender inequalities and gave us advice for the structure of the video. Prof. Dr. Christine Sempoux and Marion Brechet also gave us some feedback on our main project (B.O.T).

• Prof. Dr. Sophie Martin, Prof. Dr. Richard Benton, Linh Ho, Dinis Barros, Lisa Salamin and Gauzelin Vidovic participated in the interview.

Business plan feedback

• Prof. Dr. Davide Städler, Simon Meister, Morena Zavaletaand and Anne Headon gave us advice and feedback on the business plan project.

• Prof. Dr. Sandrine Gerber, as an expert on encapsulation of biomaterials, helped us narrow down on possible encapsulation designs for our product.

Safety

• Tobias Vornholt advised us on various aspect of safety and introduced us to multiple independent safety organisms.

showModeling Support

Finally, we would like to thank all people that helped us for the modelling part. They guided us through the different possibilities for the modeling conception, as well as helped with the learning of usage of some softwares.

• Björn Vessman and Prof. Dr. Sara Mitri gave us advice for the modelling throughout the process. Thank you!

• Imperial College 2020 iGEM team helped us with tutorials and some video calls to understand more about modelling and specifically about ODE models and the Hill equation.

• Dr. Alejandro Vignoni helped us by email and during a video call to adapt our killswitch model.

• Prof. Dr. Michael B. Elowitz kindly solved our doubts about how to rescale the different variables in his model (Elowitz et Leiber 2000).

• We exchanged some messages with Prof. Dr. Tim Rudge to better understand his software (CellModeller).

• We exchanged some messages with the Ohio State 2020 iGEM team and had a call to discuss about the killswitch modelling.

• We had a meeting with Prof. Dr. Ruud Stoof to discuss the effect of spatial space within the cell in our model.

A big thank you to our sponsors for their valuable support!