<p>With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+) where T7 promoter and lac operator could be induced with IPTG to express protein. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control.
+
<p>With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+), where T7 promoter and lac operator would be induced with IPTG. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control.
Our parts are as follows:</p>
Our parts are as follows:</p>
</section>
</section>
Latest revision as of 14:48, 26 October 2020
Parts
Overview
With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+), where T7 promoter and lac operator would be induced with IPTG. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control.
Our parts are as follows:
