Difference between revisions of "Team:CCU Taiwan/Parts"

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     <h1 class="wordcenter shadow">Safety</h1>
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     <h1 class="wordcenter shadow">Parts</h1>
 
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<nav class="section-nav">
 
     <ul>
 
     <ul>
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         <li><a href="#intro">Introduction</a></li>
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         <li><a href="#ove">Overview</a></li>
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         <li><a href="#per-saf">Personal Safety</a>
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         <li><a href="#basic">Basic Parts</a></li>
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        <li><a href="#bio-saf">Biosafety</a>
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         <li><a href="#com">Composite Parts</a></li>
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        <li><a href="#lab-saf">Labsafety</a>
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         <li>
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            <a href="#pro-saf">Project Safety</a>
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            <ul>
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                <li><a href="#nano">Nanoparticles</a></li>
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                <li><a href="#ecoli"><i>E. coli</i></a></li>
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            </ul>
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        </li>
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        <li><a href="#env-saf">Environmental Safety</a>
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     </ul>
 
     </ul>
 
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<div class="contents s12 m6 l4" id="text">
 
<div class="contents s12 m6 l4" id="text">
 
     <article>
 
     <article>
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         <section id="intro">
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         <section id="ove">
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             <h2>Introduction</h2>
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             <h2>Overview</h2>
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             <p>While we are devoted to science progress, it is also important to take safety into consideration. This document shows our compliance with the related safety rules for personal safety, biosafety, lab safety, project safety, and environmental safety.</p>
+
             <p>With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+), where T7 promoter and lac operator would be induced with IPTG. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control.
 +
Our parts are as follows:</p>
 
         </section>
 
         </section>
 
         <br>
 
         <br>
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         <section id="per-saf">
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         <section id="basic">
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             <h2>Personal Safety</h2>
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             <h2>Basic Parts</h2>
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             <p>The fatal pneumonia COVID-19 has spread across the world this year, but fortunately the situation is well-controlled in Taiwan now. However, in early February, when it appeared a potential outbreak might happen in Taiwan, we held all our discussions via online meeting to minimize the risk of infection. When the school opened again for the second semester, we measured our body temperatures and disinfected hands with alcohol before entering any building. Moreover, we always wore masks and practiced social distancing, keeping a distance at least 1.5 meters apart inside and 1 meter outside.</p>
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             <table>
 +
                <tbody>
 +
                    <tr>
 +
                        <th>Part Number</th>
 +
                        <th>Description</th>
 +
                        <th>Length (bp)</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_R0187" target="_blank">BBa_R0187</a></td>
 +
                        <td>T7 promoter (lac Irepressible)</td>
 +
                        <td>44</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2549058" target="_blank">BBa_K2549058</a></td>
 +
                        <td>HA tag</td>
 +
                        <td>27</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K3183024" target="_blank">BBa_K3183024</a></td>
 +
                        <td>HA Tag for L.reuteri</td>
 +
                        <td>27</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2382008" target="_blank">BBa_K2382008</a></td>
 +
                        <td>6X His Tag</td>
 +
                        <td>18</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_M10045" target="_blank">BBa_M10045</a></td>
 +
                        <td>Myc Tag</td>
 +
                        <td>30</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648001" target="_blank">BBa_K3648001</a></td>
 +
                        <td>C-type lectin domain family 5 member A</td>
 +
                        <td>483</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648002" target="_blank">BBa_K3648002</a></td>
 +
                        <td>Envelope protien</td>
 +
                        <td>1485</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648005" target="_blank">BBa_K3648005</a></td>
 +
                        <td>TRS-110*3</td>
 +
                        <td>102</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648006" target="_blank">BBa_K3648006</a></td>
 +
                        <td>TRS-151*8</td>
 +
                        <td>243</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648007" target="_blank">BBa_K3648007</a></td>
 +
                        <td>TRS-110R*7</td>
 +
                        <td>255</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648008" target="_blank">BBa_K3648008</a></td>
 +
                        <td>S-tag</td>
 +
                        <td>45</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648009" target="_blank">BBa_K3648009</a></td>
 +
                        <td>Thrombin site</td>
 +
                        <td>16</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648010" target="_blank">BBa_K3648010</a></td>
 +
                        <td>T7 terminator</td>
 +
                        <td>48</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648014" target="_blank">BBa_K3648014</a></td>
 +
                        <td>TRS-151*7</td>
 +
                        <td>213</td>
 +
                    </tr>
 +
                </tbody>
 +
            </table>
 
         </section>
 
         </section>
 
         <br>
 
         <br>
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         <section id="bio-saf">
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         <section id="com">
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             <h2>Biosafety</h2>
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             <h2>Composite Parts</h2>
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             <p>The organism we used in our experiment is WHO Risk Group 1, which means that the organism in its laboratory form does not easily cause human or animal disease. Besides, our team members received training courses to understand biohazards and preventive measures. In addition, we only used the dengue virus envelope protein, which is not pathogenic to humans, instead of the whole virus particle.</p>
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             <table>
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        </section>
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                <tbody>
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        <br>
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                    <tr>
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        <section id="lab-saf">
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                        <th>Part Number</th>
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            <h2>Labsafety</h2>
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                        <th>Description</th>
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            <p>Our lab is biosafety level 1, and the members wear personal protective equipment whenever conducting an experiment. All the team members also received training courses to learn safety of operating the lab instruments and accident response measures. Furthermore, the instruments are checked every day before an experiment to ensure that they are intact and functional.</p>
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                        <th>Length (bp)</th>
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        </section>
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                    </tr>
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        <br>
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                    <tr>
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        <section>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648003" target="_blank">BBa_K3648003</a></td>
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            <div id="pro-saf">
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                        <td>pET-29b(+)_CLEC5A</td>
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                <h2>Project Safety</h2>
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                        <td>655</td>
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            </div>
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                    </tr>
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            <div id="nano">
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                    <tr>
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                <h3>Nanoparticles</h3>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648004" target="_blank">BBa_K3648004</a></td>
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                <p>Nanoparticles may damage our eyes (retinas and optic nerves) when they spread in the air. They can induce damage on retinas and optic nerves, causing diseases such as retinitis and glaucoma. Therefore, we wore goggles and gloves and were careful when conducting the experiments involving gold nanoparticles. Furthermore, our reagent is covered all the time, which prevents the nanogold from escaping into the environment.</p>
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                        <td>pET-29a(+)_E protein</td>
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            </div>
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                        <td>1687</td>
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            <div id="ecoli">
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                    </tr>
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                <h3><i>E. coli</i></h3>
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                    <tr>
−
                <p>Two strains of E. coli were involved in our experiments, BL21 (DE3) and DH5α, which are low risk to human beings. After each experiment, we bleached and autoclaved the biowastes to ensure that the bacteria do not escape into the environment.</p>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648011" target="_blank">BBa_K3648011</a></td>
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            </div>
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                        <td>pET-29b(+)_TRS-110*3</td>
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        </section>
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                        <td>313</td>
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        <br>
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                    </tr>
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        <section id="env-saf">
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                    <tr>
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            <h2>Environmental Safety</h2>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648012" target="_blank">BBa_K3648012</a></td>
−
            <p>The wastes generated during the experiment were collected to prevent contaminating the environment. In terms of product, the detection reagents after the reaction may contain dengue virus, so we will not sell the product directly to the consumers but only to hospitals or the government. We also advise the users in the product manual to collect the products and dispose of them properly after use.</p>
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                        <td>pET-29b(+)_TRS-110R*7</td>
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        </section>
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                        <td>466</td>
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        <br>
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                    </tr>
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        <hr>
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                    <tr>
−
        <section id="ref">
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648013" target="_blank">BBa_K3648013</a></td>
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            <h4>Reference</h4>
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                        <td>pET-29b(+)_TRS-151*8</td>
−
            <p>World Health Organization - Laboratory biosafety manual, Third edition. World Health Organization. doi: 10.1007/SpringerReference_61629<br>
+
                        <td>414</td>
−
              Taiwan Centers for Disease Control - Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance, 4th Edition<br>
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                    </tr>
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              Biosafety level - Wikipedia<br>
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                    <tr>
−
              Zhu, Shuang; Gong, Linji; Li, Yijian; Xu, Haiwei; Gu, Zhanjun; Zhao, Yuliang - Safety<br>
+
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3648015" target="_blank">BBa_K3648015</a></td>
−
              Assessment of Nanomaterials to Eyes: An Important but Neglected Issue. doi: 10.1002/advs.201802289
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                        <td>pET-29b(+)_TRS-151*7</td>
−
             </p>
+
                        <td>384</td>
 +
                    </tr>
 +
                </tbody>
 +
             </table>
 
         </section>
 
         </section>
 
     </article>
 
     </article>

Latest revision as of 14:48, 26 October 2020

Parts

Overview

With linear array epitope technique, we have produced various length of tandem-repeated sequence, but we need the sequences which are long enough to ensure that we have enough productivity. The plasmids we used to express protein are pET-29a(+) and pET-29b(+), where T7 promoter and lac operator would be induced with IPTG. On the other hand, there are also 6X His tag on pET-29a(+) and pET-29b(+) to purify our target proteins. Furthermore, we produced E protein to do the binding affinity test and CLEC5A to be our control. Our parts are as follows:


Basic Parts

Part Number Description Length (bp)
BBa_R0187 T7 promoter (lac Irepressible) 44
BBa_K2549058 HA tag 27
BBa_K3183024 HA Tag for L.reuteri 27
BBa_K2382008 6X His Tag 18
BBa_M10045 Myc Tag 30
BBa_K3648001 C-type lectin domain family 5 member A 483
BBa_K3648002 Envelope protien 1485
BBa_K3648005 TRS-110*3 102
BBa_K3648006 TRS-151*8 243
BBa_K3648007 TRS-110R*7 255
BBa_K3648008 S-tag 45
BBa_K3648009 Thrombin site 16
BBa_K3648010 T7 terminator 48
BBa_K3648014 TRS-151*7 213

Composite Parts

Part Number Description Length (bp)
BBa_K3648003 pET-29b(+)_CLEC5A 655
BBa_K3648004 pET-29a(+)_E protein 1687
BBa_K3648011 pET-29b(+)_TRS-110*3 313
BBa_K3648012 pET-29b(+)_TRS-110R*7 466
BBa_K3648013 pET-29b(+)_TRS-151*8 414
BBa_K3648015 pET-29b(+)_TRS-151*7 384

© iGEM Team, 2020 CCU Taiwan