Difference between revisions of "Team:Vilnius-Lithuania/Contribution"

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                <div class="h2 larger">Contribution</div>
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                 <div class="h3">Parts</div>
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                 <div class="h3">blah blah</div>
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                 <p class="content-paragraph">
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                 <p class="content-paragraph">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi quis ante odio. Sed eleifend, eros at bibendum ullamcorper, nisl nisl mattis ligula, ut euismod lorem elit sit amet ipsum. Suspendisse scelerisque, odio vitae malesuada mattis, sem augue mattis velit, sit amet ornare lacus velit nec nibh. Curabitur sed lectus sapien. Suspendisse finibus urna volutpat mi consequat lacinia. In ut felis quis purus tempus gravida. Vestibulum eget gravida risus. Vivamus porta dui et nulla fringilla, nec fermentum felis placerat. Pellentesque dictum risus quis aliquam lacinia. Duis mauris arcu, rhoncus eu felis a, laoreet volutpat ipsum. Ut sed aliquam libero. Sed placerat sem nec hendrerit pellentesque.</p>
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We decided to characterise two sequences that encode the mazE (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142001" target="_blank">BBa_K2142001</a>) and mazF (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142003" target="_blank">BBa_K2142003</a>) proteins. MazE is antitoxin for the mazF toxin and mazF is antitoxin for the mazE. They both were used by iGEM NAWI Graz team in 2016. MazEF is a toxin-antitoxin relation in E. coli and other bacteria that activates programmed cell death in response to malnutrition. Cell growth and viability are not affected when MazF and MazE are coexpressed.</p>
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                 <div class="h3">super duper</div>
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                 <p class="content-paragraph">Another part that we chose to characterise was LuxS (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K091109" target="_blank">BBa_K091109</a>). LuxS is a synthase that produces 4,5-dihydroxy-2,3-pentanedione (DPD), which spontaneously forms the AI-2 signaling molecule. It was submitted by iGEM Davidson-Missouri Western in 2008. AI-2 is involved in gene expression mechanism - quorum sensing, that is regulated corresponding to cell density.</p>
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                <p class="content-paragraph">Maecenas in orci non risus fringilla maximus. Morbi id erat commodo, feugiat ante a, ullamcorper urna. Interdum et malesuada fames ac ante ipsum primis in faucibus. Mauris ligula nunc, gravida in interdum non, pellentesque eget libero. Aliquam porta purus nec arcu sodales, eget sagittis purus lacinia. Aliquam dictum augue id lacinia lacinia. Ut et elementum nunc, nec tempus lacus. Fusce vel mauris ante. Suspendisse bibendum quam tortor, vitae congue urna facilisis at. Aliquam erat volutpat. Sed sit amet magna in nunc ullamcorper porta id et dui. Vestibulum in eros nisi. Proin cursus nisl eu justo laoreet interdum. Suspendisse eu tellus vel felis iaculis posuere.</p>
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                 <div class="h3">3D test kit model</div>
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                 <div class="h3">amazing</div>
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                 <p class="content-paragraph">To fulfill our goal of developing a rapid detection strip test kit, we collaborated with “Micromolds”. The company  assisted us in the design and production of a test kit case, whose form was specially tailored to aid its function. In addition, the 3D model is freely available for other teams to 3D print, use and modify according to their own needs for LFA testing. The design is independent from the material, so using a wide variety of plastic types, including recycled plastic, is possible. Please email us to get the model.</p>
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                 <p class="content-paragraph">In porttitor, est at porta luctus, dolor mi sollicitudin libero, id placerat urna ipsum vitae metus. Vivamus ornare rhoncus est, ut condimentum sem vulputate eget. Praesent et tortor non eros molestie interdum. Vivamus ut eros quis nulla gravida placerat viverra non libero. Sed feugiat sapien feugiat, gravida risus in, luctus dolor. Donec sed nibh arcu. Sed sollicitudin lorem eu magna eleifend molestie. Nulla eu urna quis nisl aliquam feugiat. Praesent semper nec sem ut ultricies. Ut sit amet pulvinar odio. In laoreet lectus nulla, a vulputate magna porta eget. Cras ac placerat libero. Donec sollicitudin bibendum ex, at pellentesque urna.</p>
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                <div class="h3">wow</div>
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                <p class="content-paragraph">Cu esse case euripidis qui. Nonumes mediocrem vel ut. At cum meis velit, nec at dissentias cotidieque, ea modus nulla lobortis ius. Cum timeam probatus persecuti eu, sea latine debitis id, qui dolor mandamus molestiae ei. In audiam impetus eam, ius an tamquam detraxit tincidunt. Epicurei interpretaris no eos. Ne habeo scaevola sapientem sed, unum inani ubique ius ex.</p>
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    <source src="https://static.igem.org/mediawiki/2020/a/ae/T--Vilnius-Lithuania---stripemilija-.mp4" type="video/mp4">
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<a href="https://2020.igem.org/Team:Vilnius-Lithuania/Hardware" target="_blank"><button>read more about hardware</button></a>
 
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Latest revision as of 23:42, 18 December 2020

Parts

We decided to characterise two sequences that encode the mazE (BBa_K2142001) and mazF (BBa_K2142003) proteins. MazE is antitoxin for the mazF toxin and mazF is antitoxin for the mazE. They both were used by iGEM NAWI Graz team in 2016. MazEF is a toxin-antitoxin relation in E. coli and other bacteria that activates programmed cell death in response to malnutrition. Cell growth and viability are not affected when MazF and MazE are coexpressed.

Another part that we chose to characterise was LuxS (BBa_K091109). LuxS is a synthase that produces 4,5-dihydroxy-2,3-pentanedione (DPD), which spontaneously forms the AI-2 signaling molecule. It was submitted by iGEM Davidson-Missouri Western in 2008. AI-2 is involved in gene expression mechanism - quorum sensing, that is regulated corresponding to cell density.

3D test kit model

To fulfill our goal of developing a rapid detection strip test kit, we collaborated with “Micromolds”. The company assisted us in the design and production of a test kit case, whose form was specially tailored to aid its function. In addition, the 3D model is freely available for other teams to 3D print, use and modify according to their own needs for LFA testing. The design is independent from the material, so using a wide variety of plastic types, including recycled plastic, is possible. Please email us to get the model.