Difference between revisions of "Team:Vilnius-Lithuania/Contribution"

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                <div class="h3">Parts</div>
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We decided to characterise two sequences that encode the mazE (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142001" target="_blank">BBa_K2142001</a>) and mazF (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142003" target="_blank">BBa_K2142003</a>) proteins. MazE is antitoxin for the mazF toxin and mazF is antitoxin for the mazE. They both were used by iGEM NAWI Graz team in 2016. MazEF is a toxin-antitoxin relation in E. coli and other bacteria that activates programmed cell death in response to malnutrition. Cell growth and viability are not affected when MazF and MazE are coexpressed.</p>
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                <p class="content-paragraph">Another part that we chose to characterise was LuxS (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K091109" target="_blank">BBa_K091109</a>). LuxS is a synthase that produces 4,5-dihydroxy-2,3-pentanedione (DPD), which spontaneously forms the AI-2 signaling molecule. It was submitted by iGEM Davidson-Missouri Western in 2008. AI-2 is involved in gene expression mechanism - quorum sensing, that is regulated corresponding to cell density.</p>
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                <div class="h3">3D test kit model</div>
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                <p class="content-paragraph">To fulfill our goal of developing a rapid detection strip test kit, we collaborated with “Micromolds”. The company  assisted us in the design and production of a test kit case, whose form was specially tailored to aid its function. In addition, the 3D model is freely available for other teams to 3D print, use and modify according to their own needs for LFA testing. The design is independent from the material, so using a wide variety of plastic types, including recycled plastic, is possible. Please email us to get the model.</p>
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2020.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2020.igem.org/Judging/Awards"> award listed below</a>. </p>
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<a href="https://2020.igem.org/Team:Vilnius-Lithuania/Hardware" target="_blank"><button>read more about hardware</button></a>
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<h1>Contribution </h1>
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Bronze Medal Criterion #4
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<p>Make a useful contribution for future iGEM teams. Use this page to document that contribution.
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Please see the <a href="https://2020.igem.org/Judging/Medals">2020 Medals Page</a> for more information.
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<p>If you are making a contribution by adding information to an existing Part or creating a new Part, you must document your contribution on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a> for your team to be eligible for this criteria. You can use this page to link to that part and include additional information about your contribution.</p>
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Latest revision as of 23:42, 18 December 2020

Parts

We decided to characterise two sequences that encode the mazE (BBa_K2142001) and mazF (BBa_K2142003) proteins. MazE is antitoxin for the mazF toxin and mazF is antitoxin for the mazE. They both were used by iGEM NAWI Graz team in 2016. MazEF is a toxin-antitoxin relation in E. coli and other bacteria that activates programmed cell death in response to malnutrition. Cell growth and viability are not affected when MazF and MazE are coexpressed.

Another part that we chose to characterise was LuxS (BBa_K091109). LuxS is a synthase that produces 4,5-dihydroxy-2,3-pentanedione (DPD), which spontaneously forms the AI-2 signaling molecule. It was submitted by iGEM Davidson-Missouri Western in 2008. AI-2 is involved in gene expression mechanism - quorum sensing, that is regulated corresponding to cell density.

3D test kit model

To fulfill our goal of developing a rapid detection strip test kit, we collaborated with “Micromolds”. The company assisted us in the design and production of a test kit case, whose form was specially tailored to aid its function. In addition, the 3D model is freely available for other teams to 3D print, use and modify according to their own needs for LFA testing. The design is independent from the material, so using a wide variety of plastic types, including recycled plastic, is possible. Please email us to get the model.