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| Line 20: |
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| | | | |
| | <div class="contents s12 m6 l4"> | | <div class="contents s12 m6 l4"> |
| − | <section id='slide-button1'>Week1 (04/05-04/11)</section> | + | <section id='slide-button1'>Linear array epitope</section> |
| | <div class='container' id='container1' style="display: none;"> | | <div class='container' id='container1' style="display: none;"> |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>WL</h4> | | <div id="title_1"><h4>WL</h4> |
| | <hr class="style_hr">Understand the project and discuss the procedures of experiment. | | <hr class="style_hr">Understand the project and discuss the procedures of experiment. |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">Search for the principles and materials of the NS1 detection kits on the market.
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">Schedule the progress of HP.
| |
| | </div> | | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button2'>Week2 (04/12-04/18)</section> | + | <section id='slide-button2'>2. Gel extraction from agarose & polyacrylamide</section> |
| | <div class='container' id='container2' style="display: none;"> | | <div class='container' id='container2' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Find the principle of LAE and experimental procedures.</li>
| |
| − | <li>Confirm the interaction between the CLEC5A and E protein. (in progress)</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Search for the structures and binding regions of the envelope protein (E protein) and CLEC5A.</li>
| |
| − | <li>Learn how to use Chimera.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 66: |
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| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button3'>Week3 (04/19-04/25)</section> | + | <section id='slide-button3'>Plasmid extraction</section> |
| | <div class='container' id='container3' style="display: none;"> | | <div class='container' id='container3' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Optimize the experimental procedure of LAE.</li>
| |
| − | <li>Confirm the sequence and structure of E protein and design the procedure of CLEC5A expression.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">Use Chimera to simulate the docking of E protein and CLEC5A.
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 89: |
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| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button4'>Week4 (04/26-05/02)</section> | + | <section id='slide-button4'>Ligation & Transformation</section> |
| | <div class='container' id='container4' style="display: none;"> | | <div class='container' id='container4' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the TR-PCR primers. (in progress)</li>
| |
| − | <li>Confirm the interaction between the CLEC5A and E protein. (done)</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Analyze the results of Chimera.</li>
| |
| − | <li>Learn how to use Rosetta.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 116: |
Line 56: |
| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button5'>Week5 (05/03-05/09)</section> | + | <section id='slide-button5'>Colony PCR</section> |
| | <div class='container' id='container5' style="display: none;"> | | <div class='container' id='container5' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the TR-PCR primers and choose the plasmid for expression.</li>
| |
| − | <li>Design the procedure of E protein expression, E protein and CELC5A’s primers and choose the plasmid for expression. (in progress)</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Search for the principles of the NS1 antigen test and pregnancy test and design our detection kit.</li>
| |
| − | <li>Try to use the Abinitio method to produce the peptides and the FlexPepDock method to simulate peptide and protein docking by using Rosetta.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 143: |
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| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button6'>Week6 (05/10-05/16)</section> | + | <section id='slide-button6'>Protein expression (mini & large scale)</section> |
| | <div class='container' id='container6' style="display: none;"> | | <div class='container' id='container6' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the TR-PCR, E protein and CLEC5A primers. (done)</li>
| |
| − | <li>Choose the plasmid for E protein and CLEC5A expression. (E protein-pET-29a (+), CLEC5A-pET-29b (+))</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design each component of the detection pads.</li>
| |
| − | <li>Practice the FlexPepDock method to simulate peptide and protein docking.</li>
| |
| − | <li>Generate fragments which is the input of producing the peptides.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 171: |
Line 74: |
| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button7'>Week7 (05/17-05/23)</section> | + | <section id='slide-button7'>SDS PAGE</section> |
| | <div class='container' id='container7' style="display: none;"> | | <div class='container' id='container7' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Practice the instrument operating.</li>
| |
| − | <li>Culture the DH5α-pET29b, DH5α-pCMV6-CLEC5A and DH5α-pKz-V5 tag-C-CI-prM-E-(HA tag)2</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Search for the concentration of each stage of dengue virus viremia.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 198: |
Line 83: |
| | </div> | | </div> |
| | <br> | | <br> |
| − | <section id='slide-button8'>Week8 (05/24-05/30)</section> | + | <section id='slide-button8'>Western blot</section> |
| | <div class='container' id='container8' style="display: none;"> | | <div class='container' id='container8' style="display: none;"> |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Culture DH5A-pCMV6-CLEC5A.</li>
| |
| − | <li>Extract the pET-29b (+) & pCMV6-CLEC5A & pKz-V5 tag-C-CI-prM-E-(HA tag)2.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Discuss the production of the detection kit with Professor Chau, Lai-Kwan.</li>
| |
| − | <li>Search for the nature of gold nanoparticles and how to attach gold nanoparticles to the peptides.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the Mutation method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).
| |
| − | </li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| | <div id="box"> | | <div id="box"> |
| | <div id="title_1"><h4>HP</h4> | | <div id="title_1"><h4>HP</h4> |
| Line 228: |
Line 92: |
| | <li>Contact department of health in Kaohsiung city government.</li> | | <li>Contact department of health in Kaohsiung city government.</li> |
| | </ol> | | </ol> |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button9'>Week9 (05/31-06/06)</section>
| |
| − | <div class='container' id='container9' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>TR-PCR 110 & design the AD-primers.</li>
| |
| − | <li>Plasmid extraction of pCMV6-CLEC5A.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Discuss how to attach gold nanoparticles to the peptides, the size of gold nanoparticles and nonspecific adsorption of the reagent with Postdoctor Tseng, Yen-Ta.</li>
| |
| − | <li>Fill in the biosafety form.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the Mutation method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Contact Dr.Jiang, Yi-huah.</li>
| |
| − | <li>Contact Center for Dengue Prevention and Control, Tainan City Government.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button10'>Week10 (06/07-06/13)</section>
| |
| − | <div class='container' id='container10' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">Design the AD-primers. (done)
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the Mutation method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">Visit Dr.Jiang, Yi-huah.
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button11'>Week11 (06/14-06/20)</section>
| |
| − | <div class='container' id='container11' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">Optimize the conditions of TR-PCR.
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the Mutation method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">Contact Department of Health, Kaohsiung City Government.
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button12'>Week12 (06/21-06/27)</section>
| |
| − | <div class='container' id='container12' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">Optimize the conditions of TR-PCR.
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the Mutation method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Contact Center for Dengue Fever Control and Research, Kaohsiung Medical University Chung-Ho Memorial Hospital.</li>
| |
| − | <li>Contact National Mosquito-Borne Diseases Control Research Center.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button13'>Week13 (06/28-07/04)</section>
| |
| − | <div class='container' id='container13' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>TR-PCR 151(done) & TR-PCR 110.</li>
| |
| − | <li>Amplify the CLEC5A and E protein.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Confirm the source of the materials of the detection kit.</li>
| |
| − | <li>Design the home page of wiki.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the RosettaCM method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | <li>Deal with the carbohydrate on the E protein.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Record the first episode of podcast.</li>
| |
| − | <li>Build the lesson plan of education on Mingyang High School.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button14'>Week14 (07/05-07/11)</section>
| |
| − | <div class='container' id='container14' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>TR-PCR 151(done), TR-PCR 110. (done) and AD-PCR 151 and AD-PCR 110 (done).</li>
| |
| − | <li>TA-CLEC5A cloning.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Modify the component of the control line.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the RosettaCM method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | <li>Deal with the carbohydrate on the E protein.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">Virtual meetup with NCTU _Formosa iGEM.
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button15'>Week15 (07/12-07/18)</section>
| |
| − | <div class='container' id='container15' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>TA-110, TA-151, TA-151R cloning and TA-110, TA-151, TA-151R colony PCR.</li>
| |
| − | <li>TA-CLEC5A colony PCR & plasmid extraction (TA-CLEC5A) & DNA sequencing. (M13F(R)-TA-CLEC5A)</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Search for the ways of modeling by 3D printer.</li>
| |
| − | <li>Confirm the component of the control line.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the RosettaCM method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | <li>Deal with the carbohydrate on the E protein.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Visit TFDA.</li>
| |
| − | <li>Visit Center for Dengue Prevention and Control, Tainan City Government.</li>
| |
| − | <li>Visit National Mosquito-Borne Diseases Control Research Center.</li>
| |
| − | <li>Education at Cheng-He Confucian Academic Center.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button16'>Week16 (07/19-07/25)</section>
| |
| − | <div class='container' id='container16' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>TR-PCR 110R. (done)</li>
| |
| − | <li>Sequencing M13F(R)TA-151 and M13F(R)TA-151R.</li>
| |
| − | <li>Digest TA-CLEC5A, ligate CLEC5A to pET-29b (+) and transform pET-29b (+)-CLEC5A into DH5α.</li>
| |
| − | <li>pET-29b (+)-CLEC5A colony PCR, TA-E protein cloning and cultivatepET-29a (+).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the layout of wiki.</li>
| |
| − | <li>Design the project promotion video.</li>
| |
| − | <li>Arrange the content of the poster.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the RosettaCM method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptide and E protein docking.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Visit Department of Health, Kaohsiung City Government.</li>
| |
| − | <li>Visit Center for Dengue Fever Control and Research, Kaohsiung Medical University Chung-Ho Memorial Hospital.</li>
| |
| − | <li>Virtual meetup with iGEM CSMU Taiwan.</li>
| |
| − | <li>Virtual meetup with Team iGEM IISER Berhampur</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button17'>Week17 (07/26-08/01)</section>
| |
| − | <div class='container' id='container17' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>AD-PCR 110R& AD-PCR TA-151R (done), TA-110R cloning & TA-110R colony PCR.</li>
| |
| − | <li>Transform pET-29b (+)-CLEC5A into BL21(DE3) and sequencing (M13F(R)-TA-E protein).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the home page of wiki.</li>
| |
| − | <li>Design the project promotion video.</li>
| |
| − | <li>Arrange the content of the poster.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides.</li>
| |
| − | <li>Utilize the RosettaCM method and take E protein (PDB:1OAN) as a template to produce E protein (PL046).</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptide and E protein docking.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the project logo.</li>
| |
| − | <li>Record the second episode of podcast.</li>
| |
| − | <li>Compose the children storybook.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button18'>Week18 (08/02-08/08)</section>
| |
| − | <div class='container' id='container18' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Digest TA-110 and TA-151 & ligate 110 & 151 to pET-29b (+).</li>
| |
| − | <li>Sequencing (M13F(R)TA-110R) and AD-PCR TA-151R (done).</li>
| |
| − | <li>Express CLEC5A with 1μM IPTG and run SDS-PAGE.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Design the layout of the poster.</li>
| |
| − | <li>Shoot the member introduction of the project promotion video.</li>
| |
| − | <li>Calculate the force between gold nanoparticles.</li>
| |
| − | <li>Use the Abinitio method to produce the peptides(110-3 and 151-8).</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptide(110-3) and E protein(PL046) docking. (PL046).</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Virtual meetup with Dr.Liang, Shih-Yuan.</li>
| |
| − | <li>Taiwan National Education Radio interview.</li>
| |
| − | <li>Education at Cheng-He Confucian Academic Centre.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button19'>Week19 (08/09-08/15)</section>
| |
| − | <div class='container' id='container19' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Culture DH5A-pET-29b-110 & DH5A-pET-29b (+)-151.</li>
| |
| − | <li>Run the CLEC5A western blot.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Make the poster.</li>
| |
| − | <li>Shoot and edit the member introduction of the project promotion video.</li>
| |
| − | <li>Arrange the content of the poster.</li>
| |
| − | <li>Print the model for NCTU and the detection kit by using a 3D printer.</li>
| |
| − | <li>Calculate the force between gold nanoparticles.</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptide(110-3) and E protein(PL046) docking.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">Visit senior citizen service center for dengue fever informational audio recording.
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button20'>Week20 (08/16-08/22)</section>
| |
| − | <div class='container' id='container20' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>pET-29b (+)-110 colony PCR & pET-29b-151 colony PCR.</li>
| |
| − | <li>Ligate E protein into pET-29a (+) & transform pET-29a (+)-E protein into DH5α and pET-29a (+)-E protein colony PCR.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Shoot and edit the member introduction and the beginning of the project promotion video.</li>
| |
| − | <li>Calculate the force between gold nanoparticles.</li>
| |
| − | <li>Done the template of wiki.</li>
| |
| − | <li>Print the team name by using a 3D printer.</li>
| |
| − | <li>Use the Clustering method to deal with the result of the peptide.</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptide(151-8) and E protein(PL046) docking.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Record the third episode of podcast.</li>
| |
| − | <li>Taiwan iGEM conference.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button21'>Week21 (08/23-08/29)</section>
| |
| − | <div class='container' id='container21' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Optimize the procedure from digestion of TA-110 to pET-29b(+)-110 colony PCR.</li>
| |
| − | <li>Express the CLEC5A in large scale and find that protein was expressed in the pellet.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Done the member introduction and the beginning of the project promotion video.</li>
| |
| − | <li>Code the home page.</li>
| |
| − | <li>Arrange the content of the poster.</li>
| |
| − | <li>Use the Clustering method to deal with the result of the peptide.</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate protein and protein docking for NYMU.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button22'>Week22 (08/30-09/05)</section>
| |
| − | <div class='container' id='container22' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Ligate 151R to pET-29b (+).</li>
| |
| − | <li>Optimize the procedure of TA-110 with pET-29b ligation and the procedure from transformation pET-29a (+)-E protein into DH5A to protein colony PCR.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Code the wiki page of safety and sponsors.</li>
| |
| − | <li>Use the Clustering method to deal with the result of the peptide.</li>
| |
| − | <li>Use the Protein-protein Docking method to simulate peptides and E protein(PL046) docking.</li>
| |
| − | <li>Shoot and edit the project promotion video.</li>
| |
| − | <li>Do the gold nanoparticles liquidity experiment.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button23'>Week23 (09/06-09/12)</section>
| |
| − | <div class='container' id='container23' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">Optimize the condition of IPTG induction in CLEC5A expression.
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Shoot and edit the project promotion video.</li>
| |
| − | <li>Code the wiki page of description, notebook and team members.</li>
| |
| − | <li>Design and do the experiment of modifying gold nanoparticles and glass fiber.</li>
| |
| − | <li>Analysis the result of the clustering result.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button24'>Week24 (09/13-09/19)</section>
| |
| − | <div class='container' id='container24' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Shoot and edit the project promotion video.</li>
| |
| − | <li>Code the wiki page of notebook and team members.</li>
| |
| − | <li>Design and do the experiment of modifying gold nanoparticles and glass fiber.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Record the fifth episode of podcast.</li>
| |
| − | <li>Mentoring Mingdao High School.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button25'>Week25 (09/20-09/26)</section>
| |
| − | <div class='container' id='container25' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Edit the project promotion video.</li>
| |
| − | <li>Do the experiment of modifying gold nanoparticles and glass fiber.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button26'>Week26 (09/27-10/03)</section>
| |
| − | <div class='container' id='container26' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li>Code the wiki page of contribution, parts and collaborations.</li>
| |
| − | <li>Do the experiment of modifying gold nanoparticles and glass fiber.</li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button27'>Week27 (10/04-10/10)</section>
| |
| − | <div class='container' id='container27' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <section id='slide-button28'>Week28 (10/11-10/17)</section>
| |
| − | <div class='container' id='container28' style="display: none;">
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>WL</h4>
| |
| − | <hr class="style_hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>DL</h4>
| |
| − | <hr class="style_hr">
| |
| − | <ol>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | <li></li>
| |
| − | </ol>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div id="box">
| |
| − | <div id="title_1"><h4>HP</h4>
| |
| − | <hr class="style_hr">
| |
| | </div> | | </div> |
| | </div> | | </div> |
| Line 905: |
Line 169: |
| | } | | } |
| | slideButton8.addEventListener('click', SlideToggle8) | | slideButton8.addEventListener('click', SlideToggle8) |
| − |
| |
| − | let container9 = $('#container9')
| |
| − | let slideButton9 = document.getElementById('slide-button9')
| |
| − | function SlideToggle9() {
| |
| − | container9.slideToggle()
| |
| − | }
| |
| − | slideButton9.addEventListener('click', SlideToggle9)
| |
| − |
| |
| − | let container10 = $('#container10')
| |
| − | let slideButton10 = document.getElementById('slide-button10')
| |
| − | function SlideToggle10() {
| |
| − | container10.slideToggle()
| |
| − | }
| |
| − | slideButton10.addEventListener('click', SlideToggle10)
| |
| − |
| |
| − | let container11 = $('#container11')
| |
| − | let slideButton11 = document.getElementById('slide-button11')
| |
| − | function SlideToggle11() {
| |
| − | container11.slideToggle()
| |
| − | }
| |
| − | slideButton11.addEventListener('click', SlideToggle11)
| |
| − |
| |
| − | let container12 = $('#container12')
| |
| − | let slideButton12 = document.getElementById('slide-button12')
| |
| − | function SlideToggle12() {
| |
| − | container12.slideToggle()
| |
| − | }
| |
| − | slideButton12.addEventListener('click', SlideToggle12)
| |
| − |
| |
| − | let container13 = $('#container13')
| |
| − | let slideButton13 = document.getElementById('slide-button13')
| |
| − | function SlideToggle13() {
| |
| − | container13.slideToggle()
| |
| − | }
| |
| − | slideButton13.addEventListener('click', SlideToggle13)
| |
| − |
| |
| − | let container14 = $('#container14')
| |
| − | let slideButton14 = document.getElementById('slide-button14')
| |
| − | function SlideToggle14() {
| |
| − | container14.slideToggle()
| |
| − | }
| |
| − | slideButton14.addEventListener('click', SlideToggle14)
| |
| − |
| |
| − | let container15 = $('#container15')
| |
| − | let slideButton15 = document.getElementById('slide-button15')
| |
| − | function SlideToggle15() {
| |
| − | container15.slideToggle()
| |
| − | }
| |
| − | slideButton15.addEventListener('click', SlideToggle15)
| |
| − |
| |
| − | let container16 = $('#container16')
| |
| − | let slideButton16 = document.getElementById('slide-button16')
| |
| − | function SlideToggle16() {
| |
| − | container16.slideToggle()
| |
| − | }
| |
| − | slideButton16.addEventListener('click', SlideToggle16)
| |
| − |
| |
| − | let container17 = $('#container17')
| |
| − | let slideButton17 = document.getElementById('slide-button17')
| |
| − | function SlideToggle17() {
| |
| − | container17.slideToggle()
| |
| − | }
| |
| − | slideButton17.addEventListener('click', SlideToggle17)
| |
| − |
| |
| − | let container18 = $('#container18')
| |
| − | let slideButton18 = document.getElementById('slide-button18')
| |
| − | function SlideToggle18() {
| |
| − | container18.slideToggle()
| |
| − | }
| |
| − | slideButton18.addEventListener('click', SlideToggle18)
| |
| − |
| |
| − | let container19 = $('#container19')
| |
| − | let slideButton19 = document.getElementById('slide-button19')
| |
| − | function SlideToggle19() {
| |
| − | container19.slideToggle()
| |
| − | }
| |
| − | slideButton19.addEventListener('click', SlideToggle19)
| |
| − |
| |
| − | let container20 = $('#container20')
| |
| − | let slideButton20 = document.getElementById('slide-button20')
| |
| − | function SlideToggle20() {
| |
| − | container20.slideToggle()
| |
| − | }
| |
| − | slideButton20.addEventListener('click', SlideToggle20)
| |
| − |
| |
| − | let container21 = $('#container21')
| |
| − | let slideButton21 = document.getElementById('slide-button21')
| |
| − | function SlideToggle21() {
| |
| − | container21.slideToggle()
| |
| − | }
| |
| − | slideButton21.addEventListener('click', SlideToggle21)
| |
| − |
| |
| − | let container22 = $('#container22')
| |
| − | let slideButton22 = document.getElementById('slide-button22')
| |
| − | function SlideToggle22() {
| |
| − | container22.slideToggle()
| |
| − | }
| |
| − | slideButton22.addEventListener('click', SlideToggle22)
| |
| − |
| |
| − | let container23 = $('#container23')
| |
| − | let slideButton23 = document.getElementById('slide-button23')
| |
| − | function SlideToggle23() {
| |
| − | container23.slideToggle()
| |
| − | }
| |
| − | slideButton23.addEventListener('click', SlideToggle23)
| |
| − |
| |
| − | let container24 = $('#container24')
| |
| − | let slideButton24 = document.getElementById('slide-button24')
| |
| − | function SlideToggle24() {
| |
| − | container24.slideToggle()
| |
| − | }
| |
| − | slideButton24.addEventListener('click', SlideToggle24)
| |
| − |
| |
| − | let container25 = $('#container25')
| |
| − | let slideButton25 = document.getElementById('slide-button25')
| |
| − | function SlideToggle25() {
| |
| − | container25.slideToggle()
| |
| − | }
| |
| − | slideButton25.addEventListener('click', SlideToggle25)
| |
| − |
| |
| − | let container26 = $('#container26')
| |
| − | let slideButton26 = document.getElementById('slide-button26')
| |
| − | function SlideToggle26() {
| |
| − | container26.slideToggle()
| |
| − | }
| |
| − | slideButton26.addEventListener('click', SlideToggle26)
| |
| − |
| |
| − | let container27 = $('#container27')
| |
| − | let slideButton27 = document.getElementById('slide-button27')
| |
| − | function SlideToggle27() {
| |
| − | container27.slideToggle()
| |
| − | }
| |
| − | slideButton27.addEventListener('click', SlideToggle27)
| |
| − |
| |
| − | let container28 = $('#container28')
| |
| − | let slideButton28 = document.getElementById('slide-button28')
| |
| − | function SlideToggle28() {
| |
| − | container28.slideToggle()
| |
| − | }
| |
| − | slideButton28.addEventListener('click', SlideToggle28)
| |
| − |
| |
| | </script> | | </script> |
| | </body> | | </body> |
| | </html> | | </html> |
| | {{Template:CCU_Taiwan/footer}} | | {{Template:CCU_Taiwan/footer}} |
