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Revision as of 11:56, 31 July 2020
Project description
& inspiration
Defining the problem
Our Solution is really great and super
Scientific approach
Scientific approach
We are engineering the yeast Saccharomyces cerevisiae to sense presence of inflammation biomarkers (interleukins/cytokines) in human sweat. The yeast is located inside a sweat patch specifically designed for this purpose. Our SPY (Sweat Patch with Yeast) device is placed on the skin, where it continually collects sweat from the patient. The yeast expresses a colored pigment to reflect the level of a certain interleukin of choice. The vision is a modular design, allowing for easy swap of the interleukin receptor in the yeast, so the patch can be tailored to the need of the individual patient group. The yeast is powered by designed receptors that boast very high sensitivity and selectivity, coupled with an efficient pheromone pathway for signal amplification. The SPY can be photographed with a specialized app to analyze the exact level of coloration regardless of light conditions.The detailed step of the scientific development includes:
Interleukin receptor modification Human interleukin receptors are cloned into yeast, via a fusion between the extracellular receptor domain and an endogenous yeast transmembrane protein. Successful ligand-receptor binding is tested via a common split-ubiquitin signal assay.
Hijacking the yeast pheromone pathway The interleukin signal is amplified for more precise measurement by hijacking the signal amplification steps of the pheromone pathway in yeast. Endogenous G-alpha proteins are modified to induce the pathway upon ligand-receptor binding.
Signal transduction via color expression The signal pathway will, post amplification, induce the transcription of a colorful pigment that will then be expressed in the yeast cell, resulting in a clear, visual color change of the yeast colony.
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