Team:BHSF/Lab

Week1

lab finally reopen, to be honest, this is our first time stepping into such a lab, we’ve only see some equipment on our textbook before, but never really touched them or used them, so we are pretty excited but also scared.

We learned some basic rules and operations, such as how to use pipette, how to make LB medium, and how to disinfect the equipment. We are ready for the following experiment.

8.3
-PCR：vector and fragment：
-Begin transforming empty yeast vector
8.4
-finish transformation
-Gel electropheresis of pcr product
-Gel extraction of pcr product
-Cultivate the two synthesized genes
Steven Mei haven’t used the parafilm before, so he was so eager to use it! Lol

8.5
-cultivate the bacteria grown on the medium
-dna extraction
-recombine plasmid
-Make glycerol tube of the bacteria we cultivated
Kevin Yan showed his fear of supersonic in the lab, when he was walking around in the
lab, he has to figure out the pattern of the volume of sound, and rush push the machine quickly when it wasn’t making any sound.

8.6
- Michael extracted the yeast amplified yesterday
- PCR vector(PYES2) and sz2-F
-Gel electrophoresis [sz2-F,sz2-v，（products of gel extraction）,sz2,sz3（extracted by Annika the previous day, extraction proved to be successful）, four plasmids extracted by Michael)
-Gel extraction of Sz2-f、sz2-v

8.7
-PCR sz2-F
-Gel extraction of vector and the F we have PCR
-Gel electrophoresis to see whether we extracted successfully

8.8-9.12
We were busy doing HP and school work in August so we haven’t done much work. But we did similar operations and experiments.

After school began, we did experiments in the school lab, the equipment were not as advanced as before, so the chance of experiment success was lowered.

9.13
-Amplified sz2
-Plasmid extraction

9.14
- Prepared LB medium for transformation

9.15
-Annika did transformation of PYES There was no match in the lab, in order to light the alcohol lamp, Kevin went to borrow the lighter from the guard at the front gate.

9.16
-Our transformation finally succeeded! We were so encouraged!
-PCR the competent cell

9.17
-plasmid extraction
-Gel electrophoresis

9.21
-PCR again
-Gel electrophoresis
We failed many times, so we decided to design another primer.
-Amplified the bacteria that contains PYES, increase its concentration

9.22
-Made glycerol tube of the bacteria amplified yesterday

9.23
-Gel extraction of PYES
-Gel electrophoresis

9.24-now
We had lots of exams at school, but once we have time, we would go to the lab to do some tests and try to transform.

see the  Chinese version lab diary on our Wechat Official account:

LINK