Fluorescent proteins are a fundamental building block in synthetic biology research. For our “improve a part” project we decided to work with flavin-based fluorescent proteins (FbFPs), which are derived from bacterial and plant photosensory flavoproteins and commonly-used reporter genes. 
Flavoproteins have some relevant advantages compared to the green fluorescent protein – GFP. One oft the biggest advantage of FbFPs is their smaller size, compared to GFP, and their maintained functionality in oxygen-free environments. Thus, these reporter genes play a major role in bioimaging as they are independent of oxygen, unlike most other fluorescent proteins. In addition, FbFPs have been genetically improved in various ways to make them more thermostable, more ph-stable and more luminous. 
The iGEM part BBa_K3013002 codes for the protein FbFP BS2 and is a flavin mononucleotide-based fluorescent protein containing a LOV domain. The previous team Aachen 2019 improved this part by changing the reporter gene from GFP to FbFP BS2 to use it for anaerobic metabolisms. A side-effect of this exchange, was a decrease in the brightness of fluorescence.
We aim to improve this part by increasing the luminescence of FbFPs, in order to aid further iGEM teams with projects regarding for example research in anaerobic organisms and therefore require the use of FbFPs.