Team:Bielefeld-CeBiTec/Protocols
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Protocols
Protocols
- 50x TAE Stock Solution
- Chemically Competent Cells
- Colony PCR
- DNA Gel Extraction Kit (NEB)
- Electrocompetent Cells
- Error-Prone PCR using JBS Error-Prone PCR Kit (JBS)
- Gibson Assembly
- IDT Fabrication
- IMPACT Protein Purification
- LB Medium
- Ligation with T4 RNA Ligase (NEB)
- PBS Buffer
- PCR & DNA Cleanup Kit (NEB)
- PCR using OneTaq DNA Polymerase (NEB)
- PCR using Phusion DNA Polymerase (NEB)
- PCR using Q5 DNA Polymerase (NEB)
- PCR using Taq DNA Polymerase (NEB)
- Phage Amplification
- Phage Display
- Phage Purification
- Plasmid Purification Kit (Thermo Scientific)
- SDS Page
- SOC Medium
- Transformation via Electroporation
- Transformation via Heat Shock
Polymerase
| Polymerase | Manufacturer |
|---|---|
| OneTaq® DNA Polymerase | NEB |
| Phusion® High-Fidelity DNA Polymerase | NEB |
| Taq DNA Polymerases | NEB |
| Q5® High-Fidelity DNA Polymerase | NEB |