Team:FAFU-CHINA/Implementation
The least of Perfection
In the past six months, we have tried our best to optimize our project. Through brainstorming within the team, attending regional seminars, and interviewing experts in relevant fields, our project has undergone several major improvements until it is presented to you. But unfortunately, we met COVID-19 at the beginning of the year, so our experimental segment was not operational until September. So we didn't do a good job of using data to confirm the feasibility of our experiment.
But correspondingly, we have accumulated some data and future development direction through previous literature research, which enables us to have sufficient information to promote the completion of our project.
JUNCAO in Reality
Through an interview with professor Zhanxi Lin, we learned that JUNCAO has been widely used in reality. Fujian Agriculture and Forestry University has promoted the cultivation of fungus and related industries at the international level. Good progress has been made in Africa.
I think our project will be aimed at desertification areas eventually. While using fungus grass for desertification control and water and soil conservation, we can build ethanol fermentation plants in relevant areas. To achieve better ecological and economic effects.
All we need for our project is a standardized ethanol anaerobic fermenter and engineered strains that we have grown to produce.
We believe this project is easy to accomplish in the real world.
Biosafety and Challenges
When we designed the fermentation system, safety was taken into consideration, so all the strains we used were microorganisms from Group A in the iGEM White List. By doing so, we can ensure the relative safety of our project.
In addition, in industrial design, we use high temperature fermentation, which can kill the heat-resistant bacteria in the system.
By understanding the design of fermentation tank, we know that the strain in the system is not easy to escape in the fermentation process. As long as the manual operation is correct in the process of body replacement, we can guarantee the basic biosafety
In the experiment, we also encountered a lot of problems, which promoted the use of our project in reality. It has been shown in the literature that [1] Kluyveromyces marxianus has low tolerance to a variety of substrates, so how to ensure a sustained and efficient reaction rate is the direction we need to consider in the future.
Reference:
- [1]. C Wang, J Hong. Constructing microbial cell factory with thermo-tolerant yeast Kluyveromyces marxianus [J]. J Biol,20,37(01):1-10.