KOREA_HS

Designing a Hyperstable Antibody with
Cell-penetrating Peptide for Intracellular Targeting

Medal Criteria

Bronze Medal Criteria

Competition Deliverables:

#1 Wiki: The wiki was completed before the wiki freeze deadline on October 27th. Check out our wiki here
#2 Poster
#3 Presentation Video
#4 Project Promotion Video
#5 Judging Form: The judging form was submitted and confirmed. Our judging form can be seen here

Attributions:

Click here to see attributions for our project.

Project Description:

To overcome the limitations of monoclonal antibodies, CPP tagged scFv antibodies were engineered in order to create a system that will directly interfere with intracellular protein interactions… click here to see more

Contribution:

This part(BBa_K3090000) was fused to the N-terminus of scFv(P5) (part number BBa_K3090001) to design a cell-penetrating antibody fragment (part BBa_K3090002)... click here to see more

Silver Medal Criteria

Engineering Success:

Click here to see details about engineering

Collaboration:

We successfully collaborated with many different teams from all around the world, sharing our insights and thoughts about the iGEM competition. Most of our collaboration teams were collegiate teams, and they were very generous with tips and advice about parts of our project like modeling, wet lab, and outreach. We also hosted the Korea Meetup with two other high school iGEM teams in Korea as well as took part in a mentorship program with our mentor, Saniya. Furthermore, this year we started the Instagram project with many other teams for collaboration. To read more about our collaborations in detail, click here for more information.

Human Practices:

Link to Human Practices

Proposed Implementation:

Link to Implementation

Gold Medal Criteria

Integrated Human Practices:

Link to Integrated Human Practices

Our human practices work has guided our project throughout all stages of our efforts. Expert advice provided by experienced researchers has improved our experimentation process and guided us through our problems. Our surveys helped shape our education efforts to focus on delivering information on what therapeutic antibodies are, and how they can be used. We showed that our project is valuable and good to humanity, as we interviewed experts on the potential applications of our projects’ results. Our projects’ findings can apply to oncology treatments, as well as expanding the horizons of existing antibody treatments. We always prioritized real-life application in our project, as we knew that a drug had to be affordable and viable for production.

Project Modeling:

Link to Project Modeling

Through protein-protein docking our team was able to prove the effectiveness of the scFv(Ras) antibody on the molecular level and also prove the effectiveness of the antibody for lab mouse testing. Therefore the modeling supported our team to project further implementations on our scenario.

Partnership:

Link to Partnership The partnership between Imperial College London supported both teams on the improvement of both our projects in the initial and later stages of our work through two distinct meeting sessions.

Science Communication:

Link to Science Communication (Education)

Jina participated in the Korea Scholar’s Conference for Youth (KSCY) to share the proposed solution/ product of hyperstable scFv(Ras) as a unique solution to cancer. The conference gave a chance for Jina to present and receive valuable feedback and communication on the ideas to further incorporate and develop in the presentation.

Team:Korea HS/Medal Criteria