Team:Korea HS/Notebook
KOREA_HS
Designing a Hyperstable Antibody with
Cell-penetrating Peptide for Intracellular Targeting
- Contribution
- Description
- Design
- Engineering
- Experiments
- Notebook
- Partnership
- Results
- Implementation
Notebook
Whole Team
12 March 2020Team Korea_HS created21 March 20201st zoom orientation meeting
: Initial team members met for the first time. We took a look at medal criteria as a team. The professor briefly explained last year’s experiment.4 April 20202nd zoom meeting
: The professor presented a detailed explanation for last year’s project. We talked about our goal for the project.25 April 20203rd zoom meeting
: Last year’s Korea_HS team members (Dyanne, Sunwoo, Bruce) joined the zoom call and shared their iGEM experience. We checked changes iGEM made due to pandemic. We divided the team into three sub-teams: human practice, collaboration, and wiki. Team members chose leaders for the team.23 May 20204th zoom meeting
: Each sub-team presented their goals for this competition.13 June 20201st in-person meeting
: Me each other for the first time in person. Talked about last year’s experiment and what changes we are going to make this year for an improvement.2 August 20202nd in-person meeting
: Took individual portraits and team pictures. Also, our student mentor Dyanne explained about last year’s experiment and gave tips and answered questions9 August 20203rd in-person meeting
: Had a zoom meeting with Professor Jungwoo Choe and talked about modeling and assay. After the zoom session, each sub-team held their own meeting for an hour.23 August 20204th in-person meeting
: Had team lunch and each sub-team had their own discussion for two hours.13 September 20205th zoom meeting
: Finalized individual bio and chose pictures that will be to used for wiki20 September 20206th zoom meeting
: Distributed parts for presentation slides rough draft and brainstormed ideas for the general theme and color of wiki27 September 20207th zoom meeting
: Set plans for wiki building and created folders for each menu and tab4 October 20208th zoom meeting
: Finalized the rough draft for presentation slides, and chose members to present11 October 20209th zoom meeting
: Went over uncompleted informations required for wiki building, built and designed the homepage and tabs on the website18 October 202010th zoom meeting
: Set plans for presentation and poster making, finalized informations used for wiki building25 October 202011th zoom meeting
: Went over the design and layout for wiki, distributed parts for the Presentation VideoExperiment
5 July 20201st Lab
: PCR amplification of insert HRas G12V and scFv(Ras)12 July 20202nd Lab
: Transformation and recombination of plasmid to E.coli19 July 20203rd Lab
: Purification of histidine tagged scFv(Ras) and HRas23 July 20204th Lab
: SDS-PAGE of purified histidine-tagged scFv(Ras) and HRas26 July 20205th Lab
: Western Blotting30 July 2020Assay
: Binding assay of scFv(Ras) and HRasCollaboration
31 May 20201st collaboration team meeting
: Collaboration team members met for the first time28 June 20201st meeting with UNILausanne
: Each team explained their project and wet lab plans5 July 20201st meeting with DTU Denmark
: Each team explained their project and plans for public engagement12 July 20201st meeting with Queen’s University
: Each team introduced their project and gave lab suggestions18 July 20201st meeting with Imperial College London
: Participate in their mathematical modelling outreach, exchange about project progress19 July 20201st meeting with NJU China
: Exchanged team projects and inspirations. Also, received great tips on public engagement as they told us they are writing a story for children about tumor26 July 20201st iGEM mentorship meeting
: Share about our project and receive feedback on current progress with human practices work1 August 20202nd iGEM mentorship meeting
: Questions prepared prior to the meeting were answered, and Mentor Saniya gave us tips on presentation with examples2 August 20202nd meeting with DTU Denmark
: Exchanged tips on wiki and poster building8 August 2020Korea Meetup
: Team KSA_KOREA, Korea_HS, and Korea-SIS held an offline meeting. Introduced each other, explained projects, and exchanged questions.9 August 20202nd meeting with Queen’s University
: Received suggestions for the cell penetration assay and binding assay15 August 20202nd meeting with Imperial College London
: Learned the purpose for mathematical modeling and tips on homology modeling. Taught us that modeling should go beyond our experiment.22 August 20202nd meeting with UNILausanne
: Discussed about modeling and wiki building25 September 20202nd meeting with NJU China
: Discussed about WIKI guidelines and finalizing our projects.10 October 20203rd Meeting with DTU Denmark
:Discussed about adding images to WIKI website and the learning experiences of IGEM2020Human Practices
15 July 2020Interview with CELLID Discussed lab procedures and how to solve problems we faced.21 July 2020Interview with GENESCO
:Discussed advantages and disadvantages of our treatment5 August 2020Interview with ORUMRX Discussed real life applications and affected population from our research10 August 2020First survey sent out to raise awareness for our project13 September 2020Jina entered the KSCY
: The KSCY competition became a platform to share our project with other ambitious students and college professors. Due to the coronavirus, the seminar took place online.22 September 2020Pamphlet created by Yoonyee and Melissa with information on our project and antibody therapeutics.24 September 2020Schoolwide presentation about our project and what it is, and its potential implications and importance on the students.29 September 2020Pamphlet distribution by Yeonjo, informing her peers about the project and its goals, and inquiring their existing knowledge in antibody therapeutics.03 October 2020Pamphlet distributed around the school by Melissa to 9th graders, to inform them of our project.16 October 2020Second survey sent out, inquiring them about their previous knowledge in antibody related therapeutics, and their perceived dangers of these therapeutics.