Team:NJTech China/Attributions

Attributions

The Nanjing Tech University iGEM team was responsible for almost all aspects of running the team, from including purchasing supplies, coming up with the project concept, writing protocols, and executing the lab work. We would like to thank all our advisors who have assisted us throughout the project. We would also like to thank all everyone else who has helped us realize this project, be it through invaluable advice or providing DNA, strains, or other materials. These contributions have helped us enormously.

Thanks to

Team Members

Yujiao Wang

Responsible for experimental group work; Characterized the chromoprotein amajLime; Designed and constructed gene knockout plasmids containing STE5-gRNA, PRM1-gRNA, FUS1-gRNAand FUS2-gRNA; Designed and constructed the STE5 homologous recombination system for gene knockout; Provided material for presentation and communication

Yongyan Zhi

Characterized of eforRed protein; Designed and constructed gene knockout plasmids containing STE5-gRNA, PRM1-gRNA, FUS1-gRNA and FUS2-gRNA; Designed and constructed the STE5 homologous recombination system for gene knockout; Provided material for presentation and communication; Designed badge

Xinran Yin

Constructed gene knockout plasmids——pCAS(SNR52-HIS3-AmpR), pCAS(SNR52-KanR-BsaI), pRS415(GFP-CYC1);Participatedin writing wiki material

Zilu Wang

Constructed gene knockout plasmids——pCAS(SNR52-HIS3-AmpR), pCAS(SNR52-KanR-BsaI), pRS415(GFP-CYC1);Participatedin writing wiki material

Yiqing Zhang

Responsible for financial affairs；Constructed the GFP reporting pathways of three promoters(Prm1,Fig1,Fus2); Improved the Prm1 promoter (3X_reversed_PRE/6XPRE); Constructed the Ste5ΔN-CTM mutant strain of S.cerevisiae BY4741; Investigated the effects of different concentrations of α-factor mating pheromones on the growth characteristics of wild-type S.cerevisiae BY4741.

Jiacheng Li

Responsible for human practices group work; Communicated with expertsand other iGEM teams; Participated in some experiments; Provided material for presentation and communication

Xuebin Liu

Responsible for poster；Participate in science communication

Henhao Shan

Designed promotion video；Designed questionnaire.

HuiLan Wu

Participatedin science communication；Drew pictures forpromotion video, poster, wiki and presentation

Jie Zeng

Communicated with expertsand other iGEM teams; Participated in writing wiki material

Shenghua Tang

OperatedSocial Media accounts；Participatedin science communication.

Wenqin Daiyan

Communicated with experts and other iGEM teams; Participated in writing wiki material

Yicheng Lu

Responsible for wikidesign; Assembledthe material for presentation and communication. Participatedin some experiments

Siyi Zheng

Responsible for wiki coding.

Yinghao Chen

Responsible for model group work, Found a dataset of promoter efficiency for neural networks，Completed cell fusion dynamics modeling, Modeled the signaling pathway.

Yimin Gu

Read literature，Preprocess neural network model data .（Model the signal path）.

Hao Li

Realized the neural networks and the signaling pathway model using MATLAB.

Yabing Shan

Participated in human practices activities.

General Support

Thanks to our advisors for their help in team running, project guidance and experimental support. Here is their contribution.

RanLu

Provided advice on project design, team running, team members selection and grouping.

Ying Liu

Provided advice on experiments; Participated in some experiments.

Yuxin Huang

Provided advice onproject design and material preparation.

Lab support

Our Primary PI, Dr. Guannan Liu provided us with support of experiment platform, materials and equipment. She arranged the overall wet-lab plan.

Our secondary PI, Dr.Wenming Zhang helped us troubleshootour experimental design.

Xia Zhang and Changbin Gong are the teachers in charge of thelab we use in the Biochemical Engineering Experimental Teaching Center of NanjingTech University. Thanks to their support for our experimental implements and experimental materials.

Human Practices support

Thanks to professor Luo Liu, who suggested that we can study biosensors in terms of promoter response speed, strength, and dynamic range.

Thanks to professor Xin Wang, who suggested that our project can use galactose instead of pheromone to induce yeast expression, which can reduce the cost of induction.

Thanks to professor Xiaojun Ji, who proposed regulation based on promoter is a refined regulation and explains the theory of promoter engineering.

Thanks to professor Dong Liu, who suggested that we should take into account the side effects of galactose as an inducer.

Thanks to Caibing Huang, a department director in the Center for Disease Control and Prevention of Wuxi Municipal Health Bureau, who provided some methods for reducing safety risks caused by resistance gene pollution.

Thank BNU_China, BUCT_China, Jiangnan_China, NAU_China, NEU_China, NJU_China, Rochester for collaboration with our team

Other support

Qikai Zhou and Chenxi Feng are under graduates who withdrew from our team midway. Although they failed to complete the competition, thank them for their hard work in the preliminary planning of our project.

Thanks Afia Ibnat, the foreign student of our school, for engaging in designing posters to promote epidemic prevention and control in her country.

Thanks to DreamWorks Studios of Nanjing Tech University for the equipment support during the presentation video recording.

Other

Project history

We started brain storming in March this year, providing initial ideas for the project. The specific tasks arrangement and schedule planning began in May. The wet-labexperiment started in June.

About the synthetic biology course

We are a team from NanjingTech University. Our school teaches a synthetic biology course in the form of elective course, which can be taken by juniors