NEU_CHINA needed to use pET-DUET1 plasmid in their project. However, their plasmids always have low concentration and were lost during subculture. After understanding their requirements, we sent a tube of pET-DUET1 plasmid and a tube of DH5α containing pET-DUET1 to help NEU_CHINA successfully construct their recombinant plasmid in August.
The CRISPR-Cas9 plasmid pCAS (SNR52 KanR ) stored in our laboratory failed to knock out the target gene in our project. Since BNU-China’s project also involved CRISPR, we consulted them about the CRISPR plasmids. Finally, they sent the Stbl3 strain containing pML104 for Saccharomyces cerevisiae gene knockout and guided us in the design of gRNA. Although we have knocked out the target gene through homologous recombination, we plan to use pML104 in the following experiments. Meanwhile, we are continuing to communicate with BNU-China to try to work out why our pCAS plasmid cannot work properly.
After communicating with NJU-China online, we suggested them change the original CMV promoter in Tet-On system into albumin promoter to ensure specificity, so as to better express their plasmid in liver, and ultimately improve the controllability and safety of their project.
NJU-China adopted our suggestions and improved the original parts. Their project achieved the desired effect.
We participated in the SAA event organized by the University of Rochester. We created animation design works to reflect the project of our team and received positive feedback.
In September, NAU-CHINA, NWU-CHINA-B, and our team conducted online modeling communication. During the meeting, students from Northwest University and Nanjing Agricultural University put forward suggestions for the modeling work of our project. After modifying the promoter sequence by neural networks, the result is much better than before. Additionally, we also provided suggestions on the issue of earthworm placement and cost input in the cellular automata model of NAU-CHINA. This online communication is of great help to our modeling part.
In October, our team and Jiangnan_China had a modeling idea exchange via email. The advisor of Jiangnan_China provided suggestions on our neural network model and solved our questions on data processing, model parameter setting and so on.
Our team and NAU-CHINA put forward some suggestions to each other when communicating on the ethics, safety, and sustainability of the Human Practices section. NAU-CHINA suggested that our team conduct campus interviews on issues such as genetic modification and cell fusion. We also suggested that NAU-CHINA conduct campus interviews on the genetically modified section of plant cells. Consequently, we did get some meaningful opinions from campus interviews. For detailed analysis of the results, please visit our Human Practices page. https://2020.igem.org/Team:NJTech_China/Human_Practices In the later science popularization activities of the iGEM community, the ethics, safety, and sustainability issues of synthetic biology were raised. And some views gained during campus interviews were shared. We also introduced the advantages and disadvantages of genetic modification and cell fusion. The security of our team project is also widely recognized during the communication.