Team:Shanghai HS United/Notebook
Notebook
20200808
Professor Yang gave us an online presentation about the project.
20200812
All the students gathered in Shanghai, had ice-breaking activities, designed our logo, did self-introduction and selected team leader candidates.
20200813
First day in the lab, we learned safety precautions and got familiar with experimental equipment. Then did PCR to clone genes and gel extraction.
We constructed one of our plasmids and sequenced in a company.
20200817
We constructed another plasmid and sequenced in a company.
The two plasmids are transformed into E. coli competent cell.
We did fermentation experiments in a conical flask.
we also collaborated with RePhos team.
Expressed protein.
Sugar consumption and butyric acid formation were determined
20200820
Samples were taken to determine sugar consumption and butyric acid production.
We also took our team photos.
20200821
Samples were taken at anther time-point to determine sugar consumption and butyric acid production.
20200822
Analyze experimental data, summarize and answer questions.
20200823
We did live presentation on zoom software then finished our training.
Wechat: Search "2020 IGEM CounterSugar" in WeChat
School Website: https://www.xdfyz.cn/