Team:Stockholm/Parts

Parts | iGEM Stockholm

Parts

Table 1: List of parts created by iGEM Stockholm 2020.
BiobrickCodeConstructAim
BBa_K3440000APconst-RBS-LuxI-Myc-DTShow that LuxI can be expressed under constitutive promoter
BBa_K3440002BPprma-RBS-mCherry-DTTest leakiness of Pprma
BBa_K3440001CPbphR1-RBS-mCherry-DTTest bphR1 promoter leakiness and compare expression with/without PCB.
BBa_K3440003DPlux-RBS-GFPTest leakiness of Plux
BBa_K3440004EPrhl-RBS-GFPTest leakiness of Prhl
BBa_K3440005FPprma-RBS-LuxI-MycShow that LuxI can be expressed under prma promoter, and compare expression with/without PFOS.Final construct E. coli PFOS.
BBa_K3440006GPconst-RBS-RhlI-MycShow that RhlI can be expressed under constitutive promoter
BBa_K3440007HPconst-RBS-AiiA-MycShow that AiiA can be expressed under constitutive promoter
BBa_K3440008JPconst-RBS-RhlR-Myc-DTShow that RhlR can be expressed under Pconst
BBa_K3440009LPconst-RBS-bphR2-Myc-DTShow that bphR2 can be expressed under Pconst
BBa_K3440010MPbphr1-RBS-LuxI-MycShow that LuxI can be expressed under bphR1 promoter, and compare expression with/without PCB.
BBa_K3440011NPconst-RBS-GFP-DTShow that the constitutive promoter works
BBa_K3440012OPconst-RBS-RHlR-Myc-DT-Prhl-RBS-GFPShow that Prhl promoter can be activated in the presence of AHL and RhlR expression
BBa_K3440013PPconst-RBS-LuxR-MycShow that LuxR can be expressed under Pconst
BBa_K3440014QPconst-RBS-LuxR-Myc-DT-Plux-RBS-GFPShow that Plux promoter can be activated in the presence of AHL and LuxR expression
BBa_K3440015KRhlI-RBS-AiiAIntermediate part used for construction
BBa_K3440016I/Ch5Pconst-RBS-Mtrb-MycShow that MtrBcan be expressed under constitutive promoter
BBa_K3440017L3BPconst-RBS-bphR2-Myc-DT-Pbphr1-RBS-mCherryShow that bphR1 promoter can be activated by expression of bphR2 (and better with PCB)
BBa_K3440018P32Pconst-RBS-bphR2-Myc-DT-Pbphr1-RBS-LuxIFull construct for detection of PCB and expression of LuxI. Final construct PCB in E. coli
BBa_K3440019Osc1Pconst-RBS-LuxR-DT-Plux-RBS-mtrb-DTShow that MtrBcan be expressed under Plux in the presence of AHL.
BBa_K3440020Osc2Pconst-RBS-RhlR-DT-Prhl-RBS-RhlI-RBS-AiiA-RBS-GFP-DTGFP reporter of oscillation in E.coli
BBa_K3440021Osc3Plux-RBS-Mtrb-DT-Pconst-RBS-RhlR-DT-Prhl-RBS-RhlI-RBS-AiiA-RBS-LuxR-DTElectricity production under oscillatory circuit in Shewanella.