Team:Xiamen city/Notebook
Notebook
●7/18 research topic discussion (led by dr. wang from Chinese Academy of Science)
●7/25 first offline bonding
○established our team name, team logo, and team slogan
○Captain election
○Made several icebreakers to help us get to know each other and build friendship and team soul
●7/26 wet team day 1
○Learn about the use of laboratory apparatus, the principle of reagent dilution, and PCR principal
○Configure PCR reaction system and gain the DNA fragments from the PCR reaction
○Configure agarose electrophoresis and test the DNA fragments from PCR reaction
●7/27 wet team day 2
○PCR products of sod and cat genes were separated and purified from the gel extraction.
○Test the DNA concentration
○Double digestion and connection of 2 genes and plasmid
○purified DNA fragment was digested with restriction enzymes and the digested products was ligated to the linear plasmid.
●7/28 wet team day 3
○Verify the right clones by using PCR and send the verified samples for sequencing
○preparation and transformation of competent cells from Escherichia coli.
○Make solid LB plates
●7/29 wet team & dry team exchange
○Compose WeChat posts
○Advertise our market demand survey of stay-up-late alleviation products
●7/30 wet team day 4
○Verify the sequencing of sod and cat gene
○Extract plasmid
○cultivate the protein expression of bacterial strain
○IPTG overnight induction
●7/31 wet team, day 5
○Centrifugate the inducted bacterial cells
○The target proteins were released from the cells by sonication.
○Purify SOD and CAT protein by nickel chromatography
○Detection of SOD and CAT proteins by using SDS page
●8/1
○Take official portrait for wiki
●8/3 wet team, day 6
○Concentrate and desalinate the SOD and CAT protein
○The enzyme activity of SOD was examined.
●8/4 wet team, day 7
○The enzyme activity of CAT was examined.
○Review the entire experimental ideas
●8/5
○Team report speech
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