Our team, TPR_China, consists of students from several high schools, several team advisers and academic mentors. Our team members come from different cities in China and United States. The diversity makes our team unique in culture and broad in knowledge base.
Project
Hope to know new methods to contain the locust problem? Click this link to see the unique project of our team and to know more about the locust master.
Results
Successfully constructed engineering bacteria in to produce nitrilase with enough enzyme activity.
Judging
Why do we deserve metals, honors, and awards? Why are we excellent? We hope to help judges on evaluating our project.
Part
We have interesting part and we want to share them with you. Parts including several Sequences of nitrilases and plasmids constructed are applied by our team, including originals and new parts improved and collected based on the originals.
Human Practices
Besides working in the lab and conducing research, we also pay attention to human practices in order to raise social awareness of synthetic biology and advocate our projects and solutions.
Lab
This section includes notebook, general protocols, as well as lab safety during the process of experiments.
improvement
We aimed to find a broad-spectrum aromatic sensor reacting to PAN molecules.In the iGEM component library, there is already an aromatic Sensor Toolbox. We selected candidates Sensor by finding the small molecule with the best response and the similarity of the chemical structure with PAN.We constructed Xyls_sfGFP(BBa_K3599034)based on existing part BBa_K1031941.
1. Design of Xyls_sfGFP
To enhance Xyls-sensor's effect on the response to aromatic small molecules, we redesigned it. The BBa_K1031941 in the part library has been improved.Our new Part Xyls_sfGFP number is BBa_K3599034.
Fig.1 Biosensor circuit of BBa_K1031941
Fig.2 Biosensor circuit of Xyls_sfGFP（BBa_K3599034)
2. Sensor induced by small molecules
The sensor we tested was better by inducing Xyls_sfGFP and Xyls_GFP with m-toluenic acid.
Fig.3 The fluorescence of the sensor induced by m-toluenic acid
Through the corresponding small molecule sensing experiment, we can see that Xyls-sfGFP is 18 times of the Xyls-GPF(BBa_K1031941), the data proves that our improvement achieved success.
